Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy

Detalhes bibliográficos
Autor(a) principal: Hungerford, Graham
Data de Publicação: 2006
Outros Autores: Baptista, Adelina L. F., Coutinho, Paulo J. G., Castanheira, Elisabete M. S., Oliveira, M. Elisabete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/5574
Resumo: Liposomes composed of cationic lipids and neutral phospholipids have been used as vehicles for cellular delivery of DNA and drug delivery. The aim of this study is to get a better understanding of phospholipid–cationic lipid interactions that is essential for the development of this potential. We have studied the interaction between cationic lipid liposomes primarily composed of dioctadecyldimethylammonium bromide (DODAB) with two different phospholipids, dioleoyl phosphatidylethanolamine (DOPE) and soybean phosphatidylcholine (PC), as well as with cholesterol. The effect of these components on the liposome’s physical properties (microviscosity and polarity) was accessed by studying, both the steady-state and time-resolved, fluorescence anisotropy of the dye Nile Red. This information combined with analysis of the steady-state emission and fluorescence lifetime of Nile Red using the different lipids and cholesterol provided information concerning the polarity and hydration level changes in these vesicle systems along with organisational information.
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spelling Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropyDODABDOPECholesterolPCNile redFluorescence anisotropyScience & TechnologyLiposomes composed of cationic lipids and neutral phospholipids have been used as vehicles for cellular delivery of DNA and drug delivery. The aim of this study is to get a better understanding of phospholipid–cationic lipid interactions that is essential for the development of this potential. We have studied the interaction between cationic lipid liposomes primarily composed of dioctadecyldimethylammonium bromide (DODAB) with two different phospholipids, dioleoyl phosphatidylethanolamine (DOPE) and soybean phosphatidylcholine (PC), as well as with cholesterol. The effect of these components on the liposome’s physical properties (microviscosity and polarity) was accessed by studying, both the steady-state and time-resolved, fluorescence anisotropy of the dye Nile Red. This information combined with analysis of the steady-state emission and fluorescence lifetime of Nile Red using the different lipids and cholesterol provided information concerning the polarity and hydration level changes in these vesicle systems along with organisational information.ElsevierUniversidade do MinhoHungerford, GrahamBaptista, Adelina L. F.Coutinho, Paulo J. G.Castanheira, Elisabete M. S.Oliveira, M. Elisabete2006-072006-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/5574eng"Journal of photochemistry and photobiology A : chemistry". ISSN 1010-6030. 181:1 (July 2006) 99-105.1010-603010.1016/j.jphotochem.2005.11.007The original publication is available at www.sciencedirect.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:32Zoai:repositorium.sdum.uminho.pt:1822/5574Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:09.191806Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
title Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
spellingShingle Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
Hungerford, Graham
DODAB
DOPE
Cholesterol
PC
Nile red
Fluorescence anisotropy
Science & Technology
title_short Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
title_full Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
title_fullStr Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
title_full_unstemmed Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
title_sort Interaction of DODAB with neutral phospholipids and cholesterol studied using fluorescence anisotropy
author Hungerford, Graham
author_facet Hungerford, Graham
Baptista, Adelina L. F.
Coutinho, Paulo J. G.
Castanheira, Elisabete M. S.
Oliveira, M. Elisabete
author_role author
author2 Baptista, Adelina L. F.
Coutinho, Paulo J. G.
Castanheira, Elisabete M. S.
Oliveira, M. Elisabete
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Hungerford, Graham
Baptista, Adelina L. F.
Coutinho, Paulo J. G.
Castanheira, Elisabete M. S.
Oliveira, M. Elisabete
dc.subject.por.fl_str_mv DODAB
DOPE
Cholesterol
PC
Nile red
Fluorescence anisotropy
Science & Technology
topic DODAB
DOPE
Cholesterol
PC
Nile red
Fluorescence anisotropy
Science & Technology
description Liposomes composed of cationic lipids and neutral phospholipids have been used as vehicles for cellular delivery of DNA and drug delivery. The aim of this study is to get a better understanding of phospholipid–cationic lipid interactions that is essential for the development of this potential. We have studied the interaction between cationic lipid liposomes primarily composed of dioctadecyldimethylammonium bromide (DODAB) with two different phospholipids, dioleoyl phosphatidylethanolamine (DOPE) and soybean phosphatidylcholine (PC), as well as with cholesterol. The effect of these components on the liposome’s physical properties (microviscosity and polarity) was accessed by studying, both the steady-state and time-resolved, fluorescence anisotropy of the dye Nile Red. This information combined with analysis of the steady-state emission and fluorescence lifetime of Nile Red using the different lipids and cholesterol provided information concerning the polarity and hydration level changes in these vesicle systems along with organisational information.
publishDate 2006
dc.date.none.fl_str_mv 2006-07
2006-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/5574
url https://hdl.handle.net/1822/5574
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of photochemistry and photobiology A : chemistry". ISSN 1010-6030. 181:1 (July 2006) 99-105.
1010-6030
10.1016/j.jphotochem.2005.11.007
The original publication is available at www.sciencedirect.com
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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