Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/157999 |
Resumo: | PB would thank the PTNMRPhD (PD/00065/2013). VLP thanks the NIH for support (GM141086). |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptideA de novo protein designThree-helix bundleMolybdenumModels of molybdenum-containing enzymesRubredoxinTetracysteinyl coordinationSDG 13 - Climate ActionSDG 15 - Life on LandPB would thank the PTNMRPhD (PD/00065/2013). VLP thanks the NIH for support (GM141086).The rational design and functionalization of small, simple, and stable peptides scaffolds is an attractive avenue to mimic catalytic metal-centres of complex proteins, relevant for the design of metalloenzymes with environmental, biotechnological and health impacts. The de novo designed α3DIV-L21C framework has a rubredoxin-like metal binding site and was used in this work to incorporate a Mo-atom. Thermostability studies using differential scanning calorimetry showed an increase of 4 °C in the melting temperature of the Mo-α3DIV-L21C when compared to the apo-α3DIV-L21C. Circular dichroism in the visible and far-UV regions corroborated these results showing that Mo incorporation provides stability to the peptide even though there were almost no differences observed in the secondary structure. A formal reduction potential of ∼ −408 mV vs. NHE, pH 7.6 was determined. Combining electrochemical results, EPR and UV–visible data we discuss the oxidation state of the molybdenum centre in Mo-α3DIV-L21C and propose that is mainly in a Mo (VI) oxidation state.DQ - Departamento de QuímicaLAQV@REQUIMTEUCIBIO - Applied Molecular Biosciences UnitRUNBragança, Pedro M. S.Carepo, Marta S. P.Pauleta, Sofia R.Pinter, TylerElia, MaddalenaCordas, Cristina M.Moura, IsabelPecoraro, VicentMoura, José J. G.2023-09-19T22:13:07Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/157999eng0162-0134PURE: 50147275https://doi.org/10.1016/j.jinorgbio.2022.112096info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:16Zoai:run.unl.pt:10362/157999Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:56:56.475542Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| title |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| spellingShingle |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide Bragança, Pedro M. S. A de novo protein design Three-helix bundle Molybdenum Models of molybdenum-containing enzymes Rubredoxin Tetracysteinyl coordination SDG 13 - Climate Action SDG 15 - Life on Land |
| title_short |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| title_full |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| title_fullStr |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| title_full_unstemmed |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| title_sort |
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-designed α3DIV-L21C three-helical bundle peptide |
| author |
Bragança, Pedro M. S. |
| author_facet |
Bragança, Pedro M. S. Carepo, Marta S. P. Pauleta, Sofia R. Pinter, Tyler Elia, Maddalena Cordas, Cristina M. Moura, Isabel Pecoraro, Vicent Moura, José J. G. |
| author_role |
author |
| author2 |
Carepo, Marta S. P. Pauleta, Sofia R. Pinter, Tyler Elia, Maddalena Cordas, Cristina M. Moura, Isabel Pecoraro, Vicent Moura, José J. G. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DQ - Departamento de Química LAQV@REQUIMTE UCIBIO - Applied Molecular Biosciences Unit RUN |
| dc.contributor.author.fl_str_mv |
Bragança, Pedro M. S. Carepo, Marta S. P. Pauleta, Sofia R. Pinter, Tyler Elia, Maddalena Cordas, Cristina M. Moura, Isabel Pecoraro, Vicent Moura, José J. G. |
| dc.subject.por.fl_str_mv |
A de novo protein design Three-helix bundle Molybdenum Models of molybdenum-containing enzymes Rubredoxin Tetracysteinyl coordination SDG 13 - Climate Action SDG 15 - Life on Land |
| topic |
A de novo protein design Three-helix bundle Molybdenum Models of molybdenum-containing enzymes Rubredoxin Tetracysteinyl coordination SDG 13 - Climate Action SDG 15 - Life on Land |
| description |
PB would thank the PTNMRPhD (PD/00065/2013). VLP thanks the NIH for support (GM141086). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-09-19T22:13:07Z 2023-03 2023-03-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/157999 |
| url |
http://hdl.handle.net/10362/157999 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0162-0134 PURE: 50147275 https://doi.org/10.1016/j.jinorgbio.2022.112096 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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8 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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