Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/51617 |
Resumo: | Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+), 36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls (HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs in HIV/HCV+ (n = 153), HIV/HCV- (n = 169) and HIV (n = 153) patients. We found putative dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancer-related pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA signatures in PBMCs. |
id |
RCAP_0b328767e013aa0dfa9e8a75704bda0c |
---|---|
oai_identifier_str |
oai:repositorio.ul.pt:10451/51617 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV PatientsHCVHIVmicroRNAsspontaneous viral clearancehigh throughput sequenceMicro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+), 36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls (HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs in HIV/HCV+ (n = 153), HIV/HCV- (n = 169) and HIV (n = 153) patients. We found putative dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancer-related pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA signatures in PBMCs.This work has been supported by grants from Institute of Health Carlos III, [PI15CIII/00031 and PI18CIII/00020/ to AFR and VB] and the Foundation Universidad Alfonso X el Sabio-Santander [grant number 1.010.932 to AFR] and the Spanish AIDS Research Network (RD16CIII/0002/0002), and Centro de Investigación Biomédica en Red (CIBER) en Enfermedades Infecciosas (CB21/13/00044). AFR is supported by the Miguel Servet programme from Fondo de Investigación Sanitaria (ISCIII) [CP14/CIII/00010 and CPII20CIII/0001].MDPIRepositório da Universidade de LisboaValle-Millares, DanielBrochado-Kith, ÓscarMartín-Carbonero, LuzDomínguez-Domínguez, LourdesRyan, PabloDe los Santos, IgnacioDe la Fuente, SaraCastro, Juan M.Lagarde, MaríaCuevas, GuillermoMayoral-Muñoz, MarioMatarranz, MarianoDíez, VictorinoGómez-Sanz, AliciaMartínez-Román, PaulaCrespo-Bermejo, CeliaPalladino, ClaudiaMuñoz-Muñoz, MaríaJiménez-Sousa, María A.Resino, SalvadorBriz, VerónicaFernández-Rodríguez, AmandaMultidisciplinary Group of Viral Coinfection HIV/Hepatitis2022-03-07T12:29:07Z2021-11-052022-02-18T13:04:55Z2021-11-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51617engValle-Millares D, Brochado-Kith Ó, Martín-Carbonero L, Domínguez-Domínguez L, Ryan P, De los Santos I, et al. Different hcv exposure drives specific mirna profile in pbmcs of hiv patients. Biomedicines [Internet]. 2021;9(11):1627. Disponível em: https://www.mdpi.com/2227-9059/9/11/16272227-9059cv-prod-273856210.3390/biomedicines9111627info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:04Zoai:repositorio.ul.pt:10451/51617Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:41.417527Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
title |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
spellingShingle |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients Valle-Millares, Daniel HCV HIV microRNAs spontaneous viral clearance high throughput sequence |
title_short |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
title_full |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
title_fullStr |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
title_full_unstemmed |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
title_sort |
Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients |
author |
Valle-Millares, Daniel |
author_facet |
Valle-Millares, Daniel Brochado-Kith, Óscar Martín-Carbonero, Luz Domínguez-Domínguez, Lourdes Ryan, Pablo De los Santos, Ignacio De la Fuente, Sara Castro, Juan M. Lagarde, María Cuevas, Guillermo Mayoral-Muñoz, Mario Matarranz, Mariano Díez, Victorino Gómez-Sanz, Alicia Martínez-Román, Paula Crespo-Bermejo, Celia Palladino, Claudia Muñoz-Muñoz, María Jiménez-Sousa, María A. Resino, Salvador Briz, Verónica Fernández-Rodríguez, Amanda Multidisciplinary Group of Viral Coinfection HIV/Hepatitis |
author_role |
author |
author2 |
Brochado-Kith, Óscar Martín-Carbonero, Luz Domínguez-Domínguez, Lourdes Ryan, Pablo De los Santos, Ignacio De la Fuente, Sara Castro, Juan M. Lagarde, María Cuevas, Guillermo Mayoral-Muñoz, Mario Matarranz, Mariano Díez, Victorino Gómez-Sanz, Alicia Martínez-Román, Paula Crespo-Bermejo, Celia Palladino, Claudia Muñoz-Muñoz, María Jiménez-Sousa, María A. Resino, Salvador Briz, Verónica Fernández-Rodríguez, Amanda Multidisciplinary Group of Viral Coinfection HIV/Hepatitis |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Valle-Millares, Daniel Brochado-Kith, Óscar Martín-Carbonero, Luz Domínguez-Domínguez, Lourdes Ryan, Pablo De los Santos, Ignacio De la Fuente, Sara Castro, Juan M. Lagarde, María Cuevas, Guillermo Mayoral-Muñoz, Mario Matarranz, Mariano Díez, Victorino Gómez-Sanz, Alicia Martínez-Román, Paula Crespo-Bermejo, Celia Palladino, Claudia Muñoz-Muñoz, María Jiménez-Sousa, María A. Resino, Salvador Briz, Verónica Fernández-Rodríguez, Amanda Multidisciplinary Group of Viral Coinfection HIV/Hepatitis |
dc.subject.por.fl_str_mv |
HCV HIV microRNAs spontaneous viral clearance high throughput sequence |
topic |
HCV HIV microRNAs spontaneous viral clearance high throughput sequence |
description |
Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+), 36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls (HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs in HIV/HCV+ (n = 153), HIV/HCV- (n = 169) and HIV (n = 153) patients. We found putative dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancer-related pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA signatures in PBMCs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-05 2021-11-05T00:00:00Z 2022-03-07T12:29:07Z 2022-02-18T13:04:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/51617 |
url |
http://hdl.handle.net/10451/51617 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Valle-Millares D, Brochado-Kith Ó, Martín-Carbonero L, Domínguez-Domínguez L, Ryan P, De los Santos I, et al. Different hcv exposure drives specific mirna profile in pbmcs of hiv patients. Biomedicines [Internet]. 2021;9(11):1627. Disponível em: https://www.mdpi.com/2227-9059/9/11/1627 2227-9059 cv-prod-2738562 10.3390/biomedicines9111627 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134577057333248 |