Critical Aspects for Detection of Coxiella burnetii
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/4901 |
Resumo: | Coxiella burnetii is a globally distributed zoonotic γ-proteobacterium with an obligatory intracellular lifestyle. It is the causative agent of Q fever in humans and of coxiellosis among ruminants, although the agent is also detected in ticks, birds, and various other mammalian species. Requirements for intracellular multiplication together with the necessity for biosafety level 3 facilities restrict the cultivation of C. burnetii to specialized laboratories. Development of a novel medium formulation enabling axenic growth of C. burnetii has facilitated fundamental genetic studies. This review provides critical insights into direct diagnostic methods currently available for C. burnetii. It encompasses molecular detection methods, isolation, and propagation of the bacteria and its genetic characterization. Differentiation of C. burnetii from Coxiella-like organisms is an essential diagnostic prerequisite, particularly when handling and analyzing ticks. |
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Critical Aspects for Detection of Coxiella burnetiiInfecções Sistémicas e ZoonosesCoxiella burnetiiPCRAxenic CultureDirect DiagnosisGenome SequencingIsolationCoxiella burnetii is a globally distributed zoonotic γ-proteobacterium with an obligatory intracellular lifestyle. It is the causative agent of Q fever in humans and of coxiellosis among ruminants, although the agent is also detected in ticks, birds, and various other mammalian species. Requirements for intracellular multiplication together with the necessity for biosafety level 3 facilities restrict the cultivation of C. burnetii to specialized laboratories. Development of a novel medium formulation enabling axenic growth of C. burnetii has facilitated fundamental genetic studies. This review provides critical insights into direct diagnostic methods currently available for C. burnetii. It encompasses molecular detection methods, isolation, and propagation of the bacteria and its genetic characterization. Differentiation of C. burnetii from Coxiella-like organisms is an essential diagnostic prerequisite, particularly when handling and analyzing ticks.Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)Repositório Científico do Instituto Nacional de SaúdeMori, M.Mertens, K.Cutler, S.J.Santos, A.S.2018-02-01T15:32:26Z2017-012017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4901engVector Borne Zoonotic Dis. 2017 Jan;17(1):33-41. doi: 10.1089/vbz.2016.1958.1530-366710.1089/vbz.2016.1958info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:22Zoai:repositorio.insa.pt:10400.18/4901Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:16.805230Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Critical Aspects for Detection of Coxiella burnetii |
title |
Critical Aspects for Detection of Coxiella burnetii |
spellingShingle |
Critical Aspects for Detection of Coxiella burnetii Mori, M. Infecções Sistémicas e Zoonoses Coxiella burnetii PCR Axenic Culture Direct Diagnosis Genome Sequencing Isolation |
title_short |
Critical Aspects for Detection of Coxiella burnetii |
title_full |
Critical Aspects for Detection of Coxiella burnetii |
title_fullStr |
Critical Aspects for Detection of Coxiella burnetii |
title_full_unstemmed |
Critical Aspects for Detection of Coxiella burnetii |
title_sort |
Critical Aspects for Detection of Coxiella burnetii |
author |
Mori, M. |
author_facet |
Mori, M. Mertens, K. Cutler, S.J. Santos, A.S. |
author_role |
author |
author2 |
Mertens, K. Cutler, S.J. Santos, A.S. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Mori, M. Mertens, K. Cutler, S.J. Santos, A.S. |
dc.subject.por.fl_str_mv |
Infecções Sistémicas e Zoonoses Coxiella burnetii PCR Axenic Culture Direct Diagnosis Genome Sequencing Isolation |
topic |
Infecções Sistémicas e Zoonoses Coxiella burnetii PCR Axenic Culture Direct Diagnosis Genome Sequencing Isolation |
description |
Coxiella burnetii is a globally distributed zoonotic γ-proteobacterium with an obligatory intracellular lifestyle. It is the causative agent of Q fever in humans and of coxiellosis among ruminants, although the agent is also detected in ticks, birds, and various other mammalian species. Requirements for intracellular multiplication together with the necessity for biosafety level 3 facilities restrict the cultivation of C. burnetii to specialized laboratories. Development of a novel medium formulation enabling axenic growth of C. burnetii has facilitated fundamental genetic studies. This review provides critical insights into direct diagnostic methods currently available for C. burnetii. It encompasses molecular detection methods, isolation, and propagation of the bacteria and its genetic characterization. Differentiation of C. burnetii from Coxiella-like organisms is an essential diagnostic prerequisite, particularly when handling and analyzing ticks. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01 2017-01-01T00:00:00Z 2018-02-01T15:32:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4901 |
url |
http://hdl.handle.net/10400.18/4901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vector Borne Zoonotic Dis. 2017 Jan;17(1):33-41. doi: 10.1089/vbz.2016.1958. 1530-3667 10.1089/vbz.2016.1958 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE) |
publisher.none.fl_str_mv |
Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132131928047616 |