Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila

Detalhes bibliográficos
Autor(a) principal: Vaz, Filipa
Data de Publicação: 2019
Outros Autores: Kounatidis, Ilias, Covas, Gonçalo, Parton, Richard M., Harkiolaki, Maria, Davis, Ilan, Filipe, Sergio Raposo, Ligoxygakis, Petros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/146319
Resumo: BBSRC Project Grant BB/P005691/1; SFRH/BD/52207/2013 ; ONEIDA project LISBOA-01-0145-FEDER-016417; IF/01464/2013; Wellcome Trust Strategic Awards 091911/B/10/Z and 107457/Z/15/Z.
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spelling Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in DrosophilaB. subtilisDrosophilainnate immunitypeptidoglycanPGRPsS. aureusTagOBiochemistry, Genetics and Molecular Biology(all)BBSRC Project Grant BB/P005691/1; SFRH/BD/52207/2013 ; ONEIDA project LISBOA-01-0145-FEDER-016417; IF/01464/2013; Wellcome Trust Strategic Awards 091911/B/10/Z and 107457/Z/15/Z.In Drosophila, it is thought that peptidoglycan recognition proteins (PGRPs) SA and LC structurally discriminate between bacterial peptidoglycans with lysine (Lys) or diaminopimelic (DAP) acid, respectively, thus inducing differential antimicrobial transcription response. Here, we find that accessibility to PG at the cell wall plays a central role in immunity to infection. When wall teichoic acids (WTAs) are genetically removed from S. aureus (Lys type) and Bacillus subtilis (DAP type), thus increasing accessibility, the binding of both PGRPs to either bacterium is increased. PGRP-SA and -LC double mutant flies are more susceptible to infection with both WTA-less bacteria. In addition, WTA-less bacteria grow better in PGRP-SA/-LC double mutant flies. Finally, infection with WTA-less bacteria abolishes any differential activation of downstream antimicrobial transcription. Our results indicate that accessibility to cell wall PG is a major factor in PGRP-mediated immunity and may be the cause for discrimination between classes of pathogens. It is widely believed that preference in recognition of structural features of bacterial peptidoglycan (PG) drives specificity in the Drosophila antibacterial response. Vaz et al. challenge this dogma by showing that accessibility to PG plays a central role in bacterial sensing and that structural discrimination is much less stringent than previously thought.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNVaz, FilipaKounatidis, IliasCovas, GonçaloParton, Richard M.Harkiolaki, MariaDavis, IlanFilipe, Sergio RaposoLigoxygakis, Petros2022-12-16T22:07:40Z2019-05-212019-05-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/146319eng2211-1247PURE: 13463773https://doi.org/10.1016/j.celrep.2019.04.103info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:24Zoai:run.unl.pt:10362/146319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:35.287448Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
title Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
spellingShingle Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
Vaz, Filipa
B. subtilis
Drosophila
innate immunity
peptidoglycan
PGRPs
S. aureus
TagO
Biochemistry, Genetics and Molecular Biology(all)
title_short Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
title_full Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
title_fullStr Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
title_full_unstemmed Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
title_sort Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila
author Vaz, Filipa
author_facet Vaz, Filipa
Kounatidis, Ilias
Covas, Gonçalo
Parton, Richard M.
Harkiolaki, Maria
Davis, Ilan
Filipe, Sergio Raposo
Ligoxygakis, Petros
author_role author
author2 Kounatidis, Ilias
Covas, Gonçalo
Parton, Richard M.
Harkiolaki, Maria
Davis, Ilan
Filipe, Sergio Raposo
Ligoxygakis, Petros
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Vaz, Filipa
Kounatidis, Ilias
Covas, Gonçalo
Parton, Richard M.
Harkiolaki, Maria
Davis, Ilan
Filipe, Sergio Raposo
Ligoxygakis, Petros
dc.subject.por.fl_str_mv B. subtilis
Drosophila
innate immunity
peptidoglycan
PGRPs
S. aureus
TagO
Biochemistry, Genetics and Molecular Biology(all)
topic B. subtilis
Drosophila
innate immunity
peptidoglycan
PGRPs
S. aureus
TagO
Biochemistry, Genetics and Molecular Biology(all)
description BBSRC Project Grant BB/P005691/1; SFRH/BD/52207/2013 ; ONEIDA project LISBOA-01-0145-FEDER-016417; IF/01464/2013; Wellcome Trust Strategic Awards 091911/B/10/Z and 107457/Z/15/Z.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-21
2019-05-21T00:00:00Z
2022-12-16T22:07:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/146319
url http://hdl.handle.net/10362/146319
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2211-1247
PURE: 13463773
https://doi.org/10.1016/j.celrep.2019.04.103
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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