Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/109772 https://doi.org/10.1371/journal.pone.0071707 |
Resumo: | In this paper, we describe the effects of the combination of topographical, mechanical, chemical and intracellular electrical stimuli on a co-culture of fibroblasts and skeletal muscle cells. The co-culture was anisotropically grown onto an engineered micro-grooved (10 µm-wide grooves) polyacrylamide substrate, showing a precisely tuned Young's modulus (∼ 14 kPa) and a small thickness (∼ 12 µm). We enhanced the co-culture properties through intracellular stimulation produced by piezoelectric nanostructures (i.e., boron nitride nanotubes) activated by ultrasounds, thus exploiting the ability of boron nitride nanotubes to convert outer mechanical waves (such as ultrasounds) in intracellular electrical stimuli, by exploiting the direct piezoelectric effect. We demonstrated that nanotubes were internalized by muscle cells and localized in both early and late endosomes, while they were not internalized by the underneath fibroblast layer. Muscle cell differentiation benefited from the synergic combination of topographical, mechanical, chemical and nanoparticle-based stimuli, showing good myotube development and alignment towards a preferential direction, as well as high expression of genes encoding key proteins for muscle contraction (i.e., actin and myosin). We also clarified the possible role of fibroblasts in this process, highlighting their response to the above mentioned physical stimuli in terms of gene expression and cytokine production. Finally, calcium imaging-based experiments demonstrated a higher functionality of the stimulated co-cultures. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogelsAcrylic ResinsAnimalsBoron CompoundsCalciumCell DifferentiationCoculture TechniquesElastic ModulusElectric StimulationEndocytosisFibroblastsFibronectinsHumansHydrogelsMiceMicrotechnologyMyoblastsNanotubesIn this paper, we describe the effects of the combination of topographical, mechanical, chemical and intracellular electrical stimuli on a co-culture of fibroblasts and skeletal muscle cells. The co-culture was anisotropically grown onto an engineered micro-grooved (10 µm-wide grooves) polyacrylamide substrate, showing a precisely tuned Young's modulus (∼ 14 kPa) and a small thickness (∼ 12 µm). We enhanced the co-culture properties through intracellular stimulation produced by piezoelectric nanostructures (i.e., boron nitride nanotubes) activated by ultrasounds, thus exploiting the ability of boron nitride nanotubes to convert outer mechanical waves (such as ultrasounds) in intracellular electrical stimuli, by exploiting the direct piezoelectric effect. We demonstrated that nanotubes were internalized by muscle cells and localized in both early and late endosomes, while they were not internalized by the underneath fibroblast layer. Muscle cell differentiation benefited from the synergic combination of topographical, mechanical, chemical and nanoparticle-based stimuli, showing good myotube development and alignment towards a preferential direction, as well as high expression of genes encoding key proteins for muscle contraction (i.e., actin and myosin). We also clarified the possible role of fibroblasts in this process, highlighting their response to the above mentioned physical stimuli in terms of gene expression and cytokine production. Finally, calcium imaging-based experiments demonstrated a higher functionality of the stimulated co-cultures.Public Library of Science2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109772http://hdl.handle.net/10316/109772https://doi.org/10.1371/journal.pone.0071707eng1932-6203Ricotti, LeonardoFujie, ToshinoriVazão, HelenaCiofani, GianniMarotta, RobertoBrescia, RosariaFilippeschi, CarloCorradini, IreneMatteoli, MichelaMattoli, VirgilioFerreira, LinoMenciassi, Ariannainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-26T08:43:58Zoai:estudogeral.uc.pt:10316/109772Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:55.205120Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
title |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
spellingShingle |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels Ricotti, Leonardo Acrylic Resins Animals Boron Compounds Calcium Cell Differentiation Coculture Techniques Elastic Modulus Electric Stimulation Endocytosis Fibroblasts Fibronectins Humans Hydrogels Mice Microtechnology Myoblasts Nanotubes |
title_short |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
title_full |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
title_fullStr |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
title_full_unstemmed |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
title_sort |
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels |
author |
Ricotti, Leonardo |
author_facet |
Ricotti, Leonardo Fujie, Toshinori Vazão, Helena Ciofani, Gianni Marotta, Roberto Brescia, Rosaria Filippeschi, Carlo Corradini, Irene Matteoli, Michela Mattoli, Virgilio Ferreira, Lino Menciassi, Arianna |
author_role |
author |
author2 |
Fujie, Toshinori Vazão, Helena Ciofani, Gianni Marotta, Roberto Brescia, Rosaria Filippeschi, Carlo Corradini, Irene Matteoli, Michela Mattoli, Virgilio Ferreira, Lino Menciassi, Arianna |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ricotti, Leonardo Fujie, Toshinori Vazão, Helena Ciofani, Gianni Marotta, Roberto Brescia, Rosaria Filippeschi, Carlo Corradini, Irene Matteoli, Michela Mattoli, Virgilio Ferreira, Lino Menciassi, Arianna |
dc.subject.por.fl_str_mv |
Acrylic Resins Animals Boron Compounds Calcium Cell Differentiation Coculture Techniques Elastic Modulus Electric Stimulation Endocytosis Fibroblasts Fibronectins Humans Hydrogels Mice Microtechnology Myoblasts Nanotubes |
topic |
Acrylic Resins Animals Boron Compounds Calcium Cell Differentiation Coculture Techniques Elastic Modulus Electric Stimulation Endocytosis Fibroblasts Fibronectins Humans Hydrogels Mice Microtechnology Myoblasts Nanotubes |
description |
In this paper, we describe the effects of the combination of topographical, mechanical, chemical and intracellular electrical stimuli on a co-culture of fibroblasts and skeletal muscle cells. The co-culture was anisotropically grown onto an engineered micro-grooved (10 µm-wide grooves) polyacrylamide substrate, showing a precisely tuned Young's modulus (∼ 14 kPa) and a small thickness (∼ 12 µm). We enhanced the co-culture properties through intracellular stimulation produced by piezoelectric nanostructures (i.e., boron nitride nanotubes) activated by ultrasounds, thus exploiting the ability of boron nitride nanotubes to convert outer mechanical waves (such as ultrasounds) in intracellular electrical stimuli, by exploiting the direct piezoelectric effect. We demonstrated that nanotubes were internalized by muscle cells and localized in both early and late endosomes, while they were not internalized by the underneath fibroblast layer. Muscle cell differentiation benefited from the synergic combination of topographical, mechanical, chemical and nanoparticle-based stimuli, showing good myotube development and alignment towards a preferential direction, as well as high expression of genes encoding key proteins for muscle contraction (i.e., actin and myosin). We also clarified the possible role of fibroblasts in this process, highlighting their response to the above mentioned physical stimuli in terms of gene expression and cytokine production. Finally, calcium imaging-based experiments demonstrated a higher functionality of the stimulated co-cultures. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/109772 http://hdl.handle.net/10316/109772 https://doi.org/10.1371/journal.pone.0071707 |
url |
http://hdl.handle.net/10316/109772 https://doi.org/10.1371/journal.pone.0071707 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134140853911552 |