Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.5/23582 |
Resumo: | Research Areas: Agriculture ; Veterinary Sciences ; Zoology |
| id |
RCAP_0d0b815773264d85ef54e77143276495 |
|---|---|
| oai_identifier_str |
oai:www.repository.utl.pt:10400.5/23582 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned PigletsPigletsAmino acidsImmunityChallengeResearch Areas: Agriculture ; Veterinary Sciences ; ZoologyIn order to investigate the effect of a dietary amino acid mixture supplementation in lipopolysaccharide (LPS)-challenged weaned piglets, twenty-seven 28-day-old (8.2 ± 1.0 kg) newly weaned piglets were randomly allocated to one of three experimental treatments for five weeks. Diet 1: a CTRL treatment. Diet 2: an LPS treatment, where piglets were intraperitoneally administered LPS (25 μg/kg) on day 7. Diet 3: an LPS+MIX treatment, where piglets were intraperitoneally administered LPS on day 7 and fed a diet supplemented with a mixture of 0.3% of arginine, branched-chain amino acids (leucine, valine, and isoleucine), and cystine (MIX). Blood samples were drawn on day 10 and day 35, and serum was analysed for selected chemical parameters and proteomics. The LPS and LPS+MIX groups exhibited an increase in haptoglobin concentrations on day 10. The LPS group showed an increased cortisol concentration, while this concentration was reduced in the LPS+MIX group compared to the control group. Similarly, the LPS+MIX group showed a decreased haptoglobin concentration on day 35 compared to the two other groups. Immunoglobulin concentrations were affected by treatments. Indeed, on day 10, the concentrations of IgG and IgM were decreased by the LPS challenge, as illustrated by the lower concentrations of these two immunoglobulins in the LPS group compared to the control group. In addition, the supplementation with the amino acid mixture in the LPS+MIX further decreased IgG and increased IgM concentrations compared to the LPS group. Although a proteomics approach did not reveal important alterations in the protein profile in response to treatments, LPS-challenged piglets had an increase in proteins linked to the immune response, when compared to piglets supplemented with the amino acid mixture. Overall, data indicate that LPS-challenged piglets supplemented with this amino acid mixture are more protected against the detrimental effects of LPS.MDPIRepositório da Universidade de LisboaPrates, José A.MFreire, JoãoAlmeida, AndréMartins, Cátia FalcãoRibeiro, David M.Osorio, HugoPinho, MárioLopes, Paula AlexandraCorreia, Jorge Manuel de JesusPinto, Rui M. A.2022-02-17T21:50:15Z2021-04-162021-04-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23582engPrates JAM, Freire JPB, de Almeida AM, Martins C, Ribeiro DM, Osorio H, Pinho MAS, Lopes PA, Correia JMJ, Pinto RMA. 2021. Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets. Animals, 11(4):1143. Doi: 10.3390/ani110411432076-261510.3390/ani11041143info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:09Zoai:www.repository.utl.pt:10400.5/23582Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:47.227971Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| title |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| spellingShingle |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets Prates, José A.M Piglets Amino acids Immunity Challenge |
| title_short |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| title_full |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| title_fullStr |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| title_full_unstemmed |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| title_sort |
Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets |
| author |
Prates, José A.M |
| author_facet |
Prates, José A.M Freire, João Almeida, André Martins, Cátia Falcão Ribeiro, David M. Osorio, Hugo Pinho, Mário Lopes, Paula Alexandra Correia, Jorge Manuel de Jesus Pinto, Rui M. A. |
| author_role |
author |
| author2 |
Freire, João Almeida, André Martins, Cátia Falcão Ribeiro, David M. Osorio, Hugo Pinho, Mário Lopes, Paula Alexandra Correia, Jorge Manuel de Jesus Pinto, Rui M. A. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Prates, José A.M Freire, João Almeida, André Martins, Cátia Falcão Ribeiro, David M. Osorio, Hugo Pinho, Mário Lopes, Paula Alexandra Correia, Jorge Manuel de Jesus Pinto, Rui M. A. |
| dc.subject.por.fl_str_mv |
Piglets Amino acids Immunity Challenge |
| topic |
Piglets Amino acids Immunity Challenge |
| description |
Research Areas: Agriculture ; Veterinary Sciences ; Zoology |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-04-16 2021-04-16T00:00:00Z 2022-02-17T21:50:15Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/23582 |
| url |
http://hdl.handle.net/10400.5/23582 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Prates JAM, Freire JPB, de Almeida AM, Martins C, Ribeiro DM, Osorio H, Pinho MAS, Lopes PA, Correia JMJ, Pinto RMA. 2021. Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-Challenged Weaned Piglets. Animals, 11(4):1143. Doi: 10.3390/ani11041143 2076-2615 10.3390/ani11041143 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799131171860250624 |