3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect

Detalhes bibliográficos
Autor(a) principal: Queirós, Odília
Data de Publicação: 2012
Outros Autores: Preto, Ana, Pacheco, António, Pinheiro, Céline, Silva, João Azevedo, Moreira, Roxana, Pedro, Madalena, Ko, Young, Pedersen, Peter, Baltazar, Fátima, Casal, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/21366
Resumo: The authors are deeply grateful to Professor André Goffeau (Université Catholique de Louvain-la-Neuve, Belgium) for his contagious vision, valuable discussions at the Conference on 3-BP held in Brussels, 29 October–2 November, 2011 and critical reviewing of the manuscript. The authors also thank Dr. Rita Reis for her technical support in microscopy and image analysis.
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spelling 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect3-bromopyruvateButyrateMonocarboxylate transportersWarburg effectScience & TechnologyThe authors are deeply grateful to Professor André Goffeau (Université Catholique de Louvain-la-Neuve, Belgium) for his contagious vision, valuable discussions at the Conference on 3-BP held in Brussels, 29 October–2 November, 2011 and critical reviewing of the manuscript. The authors also thank Dr. Rita Reis for her technical support in microscopy and image analysis.Most malignant tumors exhibit theWarburg effect, which consists in increased glycolysis rates with production of lactate, even in the presence of oxygen. Monocarboxylate transporters (MCTs), maintain these glycolytic rates, by mediating the influx and/or efflux of lactate and are overexpressed in several cancer cell types. The lactate and pyruvate analogue 3-bromopyruvate (3-BP) is an inhibitor of the energy metabolism, which has been proposed as a specific antitumor agent. In the present study, we aimed at determining the effect of 3-BP in breast cancer cells and evaluated the putative role of MCTs on this effect. Our results showed that the three breast cancer cell lines used presented different sensitivities to 3-BP: ZR-75-1 ER (+)>MCF-7 ER (+)>SK-BR-3 ER (−).We also demonstrated that 3-BP reduced lactate production, induced cell morphological alterations and increased apoptosis. The effect of 3-BP appears to be cytotoxic rather than cytostatic, as a continued decrease in cell viability was observed after removal of 3-BP. We showed that pre-incubation with butyrate enhanced significantly 3-BP cytotoxicity, especially in the most resistant breast cancer cell line, SK-BR-3. We observed that butyrate treatment induced localization of MCT1 in the plasma membrane as well as overexpression of MCT4 and its chaperone CD147. Our results thus indicate that butyrate pre-treatment potentiates the effect of 3-BP, most probably by increasing the rates of 3-BP transport through MCT1/4. This study supports the potential use of butyrate as adjuvant of 3-BP in the treatment of breast cancer resistant cells, namely ER (−).This work was financed by Fundos FEDER, Programa Operacional Fatores de Competitividade – COMPETE and by Portuguese National Funds, FCT – Fundação para a Ciência e a Tecnologia, in the scope of the project PEst-C/BIA/UI4050/2011. PLP was supported in part by NIH grant CA 10951.SpringerUniversidade do MinhoQueirós, OdíliaPreto, AnaPacheco, AntónioPinheiro, CélineSilva, João AzevedoMoreira, RoxanaPedro, MadalenaKo, YoungPedersen, PeterBaltazar, FátimaCasal, Margarida2012-022012-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/21366eng0145-479X10.1007/s10863-012-9418-322350013http://link.springer.com/article/10.1007%2Fs10863-012-9418-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:12Zoai:repositorium.sdum.uminho.pt:1822/21366Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:24.933315Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
title 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
spellingShingle 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
Queirós, Odília
3-bromopyruvate
Butyrate
Monocarboxylate transporters
Warburg effect
Science & Technology
title_short 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
title_full 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
title_fullStr 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
title_full_unstemmed 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
title_sort 3-bromopyruvate, butyrate, monocarboxylate transporters, warburg effect
author Queirós, Odília
author_facet Queirós, Odília
Preto, Ana
Pacheco, António
Pinheiro, Céline
Silva, João Azevedo
Moreira, Roxana
Pedro, Madalena
Ko, Young
Pedersen, Peter
Baltazar, Fátima
Casal, Margarida
author_role author
author2 Preto, Ana
Pacheco, António
Pinheiro, Céline
Silva, João Azevedo
Moreira, Roxana
Pedro, Madalena
Ko, Young
Pedersen, Peter
Baltazar, Fátima
Casal, Margarida
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Queirós, Odília
Preto, Ana
Pacheco, António
Pinheiro, Céline
Silva, João Azevedo
Moreira, Roxana
Pedro, Madalena
Ko, Young
Pedersen, Peter
Baltazar, Fátima
Casal, Margarida
dc.subject.por.fl_str_mv 3-bromopyruvate
Butyrate
Monocarboxylate transporters
Warburg effect
Science & Technology
topic 3-bromopyruvate
Butyrate
Monocarboxylate transporters
Warburg effect
Science & Technology
description The authors are deeply grateful to Professor André Goffeau (Université Catholique de Louvain-la-Neuve, Belgium) for his contagious vision, valuable discussions at the Conference on 3-BP held in Brussels, 29 October–2 November, 2011 and critical reviewing of the manuscript. The authors also thank Dr. Rita Reis for her technical support in microscopy and image analysis.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
2012-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/21366
url http://hdl.handle.net/1822/21366
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0145-479X
10.1007/s10863-012-9418-3
22350013
http://link.springer.com/article/10.1007%2Fs10863-012-9418-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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