Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer

Detalhes bibliográficos
Autor(a) principal: Araújo, José E.
Data de Publicação: 2018
Outros Autores: López-Fernández, H., Diniz, M. S., Baltazar, Pedro M., Pinheiro, Luís Campos, da Silva, Fernando Calais, Carrascal, Mylène, Videira, Paula, Santos, H. M., Capelo, J. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1016/j.talanta.2017.11.063
Resumo: The PROTEOMASS Scientific Society is acknowledged by the funding provided to the Biological Mass Spectrometry Isabel Moura. Authors acknowledge the funding provided by UCIBIO, Unidade de Ciencias Biomoleculares Aplicadas, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728) and to the Associate Laboratory for Green Chemistry LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). J. E. Araujo acknowledges the financial support given by the Portuguese Foundation for Science and Technology under doctoral grant number SFRH/BD/109201/2015. H. Lopez-Fernandez is supported by a post-doctoral fellowship from Xunta de Galicia. H.M.S. is funded by the FCT Investigator Programme 2015 (IF/00007/2015). The Nova Medical School is also acknowledged for financial support under project "Discovery of biomarkers for bladder carcinoma diagnosis" - NOVA health programme 2015.
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spelling Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer2D-SDS-PAGEBladder cancerLUTSMALDI-TOF-MSProtein equalizationS2PChemistry(all)SDG 3 - Good Health and Well-beingThe PROTEOMASS Scientific Society is acknowledged by the funding provided to the Biological Mass Spectrometry Isabel Moura. Authors acknowledge the funding provided by UCIBIO, Unidade de Ciencias Biomoleculares Aplicadas, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728) and to the Associate Laboratory for Green Chemistry LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). J. E. Araujo acknowledges the financial support given by the Portuguese Foundation for Science and Technology under doctoral grant number SFRH/BD/109201/2015. H. Lopez-Fernandez is supported by a post-doctoral fellowship from Xunta de Galicia. H.M.S. is funded by the FCT Investigator Programme 2015 (IF/00007/2015). The Nova Medical School is also acknowledged for financial support under project "Discovery of biomarkers for bladder carcinoma diagnosis" - NOVA health programme 2015.This study aimed to assess the benefits of dithiothreitol (DTT)-based sample treatment for protein equalization to assess potential biomarkers for bladder cancer. The proteome of plasma samples of patients with bladder carcinoma, patients with lower urinary tract symptoms (LUTS) and healthy volunteers, was equalized with dithiothreitol (DTT) and compared. The equalized proteomes were interrogated using two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry. Six proteins, namely serum albumin, gelsolin, fibrinogen gamma chain, Ig alpha-1 chain C region, Ig alpha-2 chain C region and haptoglobin, were found dysregulated in at least 70% of bladder cancer patients when compared with a pool of healthy individuals. One protein, serum albumin, was found overexpressed in 70% of the patients when the equalized proteome of the healthy pool was compared with the equalized proteome of the LUTS patients. The pathways modified by the proteins differentially expressed were analyzed using Cytoscape. The method here presented is fast, cheap, of easy application and it matches the analytical minimalism rules as outlined by Halls. Orthogonal validation was done using western-blot. Overall, DTT-based protein equalization is a promising methodology in bladder cancer research.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)DCV - Departamento de Ciências da VidaRUNAraújo, José E.López-Fernández, H.Diniz, M. S.Baltazar, Pedro M.Pinheiro, Luís Camposda Silva, Fernando CalaisCarrascal, MylèneVideira, PaulaSantos, H. M.Capelo, J. L.2019-04-16T22:13:35Z2018-04-012018-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttps://doi.org/10.1016/j.talanta.2017.11.063eng0039-9140PURE: 3696027http://www.scopus.com/inward/record.url?scp=85038094574&partnerID=8YFLogxKhttps://doi.org/10.1016/j.talanta.2017.11.063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:31:48Zoai:run.unl.pt:10362/66872Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:35.344293Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
title Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
spellingShingle Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
Araújo, José E.
2D-SDS-PAGE
Bladder cancer
LUTS
MALDI-TOF-MS
Protein equalization
S2P
Chemistry(all)
SDG 3 - Good Health and Well-being
title_short Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
title_full Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
title_fullStr Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
title_full_unstemmed Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
title_sort Dithiothreitol-based protein equalization technology to unravel biomarkers for bladder cancer
author Araújo, José E.
author_facet Araújo, José E.
López-Fernández, H.
Diniz, M. S.
Baltazar, Pedro M.
Pinheiro, Luís Campos
da Silva, Fernando Calais
Carrascal, Mylène
Videira, Paula
Santos, H. M.
Capelo, J. L.
author_role author
author2 López-Fernández, H.
Diniz, M. S.
Baltazar, Pedro M.
Pinheiro, Luís Campos
da Silva, Fernando Calais
Carrascal, Mylène
Videira, Paula
Santos, H. M.
Capelo, J. L.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Araújo, José E.
López-Fernández, H.
Diniz, M. S.
Baltazar, Pedro M.
Pinheiro, Luís Campos
da Silva, Fernando Calais
Carrascal, Mylène
Videira, Paula
Santos, H. M.
Capelo, J. L.
dc.subject.por.fl_str_mv 2D-SDS-PAGE
Bladder cancer
LUTS
MALDI-TOF-MS
Protein equalization
S2P
Chemistry(all)
SDG 3 - Good Health and Well-being
topic 2D-SDS-PAGE
Bladder cancer
LUTS
MALDI-TOF-MS
Protein equalization
S2P
Chemistry(all)
SDG 3 - Good Health and Well-being
description The PROTEOMASS Scientific Society is acknowledged by the funding provided to the Biological Mass Spectrometry Isabel Moura. Authors acknowledge the funding provided by UCIBIO, Unidade de Ciencias Biomoleculares Aplicadas, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728) and to the Associate Laboratory for Green Chemistry LAQV which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). J. E. Araujo acknowledges the financial support given by the Portuguese Foundation for Science and Technology under doctoral grant number SFRH/BD/109201/2015. H. Lopez-Fernandez is supported by a post-doctoral fellowship from Xunta de Galicia. H.M.S. is funded by the FCT Investigator Programme 2015 (IF/00007/2015). The Nova Medical School is also acknowledged for financial support under project "Discovery of biomarkers for bladder carcinoma diagnosis" - NOVA health programme 2015.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
2018-04-01T00:00:00Z
2019-04-16T22:13:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.talanta.2017.11.063
url https://doi.org/10.1016/j.talanta.2017.11.063
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0039-9140
PURE: 3696027
http://www.scopus.com/inward/record.url?scp=85038094574&partnerID=8YFLogxK
https://doi.org/10.1016/j.talanta.2017.11.063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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