Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+

Detalhes bibliográficos
Autor(a) principal: Lima, Marta
Data de Publicação: 2012
Outros Autores: Dias, Alberto Carlos Pires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/18371
Resumo: The biphasic oxidative burst induced by Phaeomoniella chlamydospora extract (Pce) in Vitis vinifera (Vv) cell suspensions was investigated. Treatment of cell suspensions with diphenyleneiodonium chloride, an inhibitor of NADPH oxidase, prevented the Pce-induced biphasic reactive oxygen species (ROS) accumulation, suggesting that NADPH oxidase is the primary ROS source in the oxidative burst induced by Pce elicitation of Vv cells. The role of Ca2+ in the oxidative burst was also investigated using a Ca2+ chelator and several Ca2+ channel blockers. The treatment of Vv cell suspensions with the Ca2+ chelator ethylene glycol-bis(2-aminoethylether)-N, N, N’; N’-tetraacetic acid (EGTA) completely inhibited Pce-induced ROS accumulation, suggesting that Ca2+ availability is necessary for occurrence of the induced oxidative burst. However, only the Ca2+ channel blocker ruthenium red strongly inhibited the Pce-induced ROS accumulation, suggesting that the specific Ca2+ channel types from which Ca2+ influx is originated also play an important role in the Pce-induced oxidative burst. Furthermore, Ca2+ availability seems to be necessary for the Pce-induced activity of NADPH oxidase.
id RCAP_0ec4808b264e2fcd89656da9645d6c7b
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/18371
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+Esca diseaseMeaslesReactive oxygen speciesGrapevineIn vitro culturesCa2+ chelatorsCa2+ channel blockersCa channel blockers 2+Ca chelators 2+Science & TechnologyThe biphasic oxidative burst induced by Phaeomoniella chlamydospora extract (Pce) in Vitis vinifera (Vv) cell suspensions was investigated. Treatment of cell suspensions with diphenyleneiodonium chloride, an inhibitor of NADPH oxidase, prevented the Pce-induced biphasic reactive oxygen species (ROS) accumulation, suggesting that NADPH oxidase is the primary ROS source in the oxidative burst induced by Pce elicitation of Vv cells. The role of Ca2+ in the oxidative burst was also investigated using a Ca2+ chelator and several Ca2+ channel blockers. The treatment of Vv cell suspensions with the Ca2+ chelator ethylene glycol-bis(2-aminoethylether)-N, N, N’; N’-tetraacetic acid (EGTA) completely inhibited Pce-induced ROS accumulation, suggesting that Ca2+ availability is necessary for occurrence of the induced oxidative burst. However, only the Ca2+ channel blocker ruthenium red strongly inhibited the Pce-induced ROS accumulation, suggesting that the specific Ca2+ channel types from which Ca2+ influx is originated also play an important role in the Pce-induced oxidative burst. Furthermore, Ca2+ availability seems to be necessary for the Pce-induced activity of NADPH oxidase.Fundação para a Ciência e a Tecnologia (FCT)WileyUniversidade do MinhoLima, MartaDias, Alberto Carlos Pires20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/18371eng1439-043410.1111/j.1439-0434.2011.01871.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:43Zoai:repositorium.sdum.uminho.pt:1822/18371Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:34.530807Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
title Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
spellingShingle Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
Lima, Marta
Esca disease
Measles
Reactive oxygen species
Grapevine
In vitro cultures
Ca2+ chelators
Ca2+ channel blockers
Ca channel blockers 2+
Ca chelators 2+
Science & Technology
title_short Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
title_full Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
title_fullStr Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
title_full_unstemmed Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
title_sort Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell suspensions : role of NADPH oxidase and Ca2+
author Lima, Marta
author_facet Lima, Marta
Dias, Alberto Carlos Pires
author_role author
author2 Dias, Alberto Carlos Pires
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lima, Marta
Dias, Alberto Carlos Pires
dc.subject.por.fl_str_mv Esca disease
Measles
Reactive oxygen species
Grapevine
In vitro cultures
Ca2+ chelators
Ca2+ channel blockers
Ca channel blockers 2+
Ca chelators 2+
Science & Technology
topic Esca disease
Measles
Reactive oxygen species
Grapevine
In vitro cultures
Ca2+ chelators
Ca2+ channel blockers
Ca channel blockers 2+
Ca chelators 2+
Science & Technology
description The biphasic oxidative burst induced by Phaeomoniella chlamydospora extract (Pce) in Vitis vinifera (Vv) cell suspensions was investigated. Treatment of cell suspensions with diphenyleneiodonium chloride, an inhibitor of NADPH oxidase, prevented the Pce-induced biphasic reactive oxygen species (ROS) accumulation, suggesting that NADPH oxidase is the primary ROS source in the oxidative burst induced by Pce elicitation of Vv cells. The role of Ca2+ in the oxidative burst was also investigated using a Ca2+ chelator and several Ca2+ channel blockers. The treatment of Vv cell suspensions with the Ca2+ chelator ethylene glycol-bis(2-aminoethylether)-N, N, N’; N’-tetraacetic acid (EGTA) completely inhibited Pce-induced ROS accumulation, suggesting that Ca2+ availability is necessary for occurrence of the induced oxidative burst. However, only the Ca2+ channel blocker ruthenium red strongly inhibited the Pce-induced ROS accumulation, suggesting that the specific Ca2+ channel types from which Ca2+ influx is originated also play an important role in the Pce-induced oxidative burst. Furthermore, Ca2+ availability seems to be necessary for the Pce-induced activity of NADPH oxidase.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/18371
url http://hdl.handle.net/1822/18371
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1439-0434
10.1111/j.1439-0434.2011.01871.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132548421386240

Registros relacionados