Chronic ketamine administration impairs mitochondrial complex I in the rat liver
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/12282 |
Resumo: | Ketamine can induce hepatotoxicity which has been suggested to be dependent on mitochondrial impairment. This study investigated the long-term effects of chronic low-dose ketamine on liver mitochondrial function, oxidative stress parameters, liver histology and glycogen content. Adult rats were administered with saline or ketamine (5 or 10 mg/kg) twice a day for a fourteen-day period in order to mimic chronic treatments. Effects between groups were compared ten days after the treatment had ended. Liver mitochondrial function was monitored in isolated mitochondrial extracts through evaluation of respiration parameters and activity of respiratory complexes, as well as oxidative stress, through lipid peroxidation, protein oxidation and superoxide dismutase activity. The hepatic histology and liver glycogen content were also evaluated. Ketamine groups showed a decreased evolution in body weight gains during the treatment period. Ketamine had no effect either on serum liver enzymes or on the oxidative stress parameters of liver mitochondria. Ketamine decreased the hepatic glycogen content, inhibited mitochondrial complex I and oxygen consumption when glutamate–malate substrate was used. These findings reflect a long-term mitochondrial bioenergetic deterioration induced by ketamine, which may explain the increased susceptibility of some patients to its prolonged or repeated use. |
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Chronic ketamine administration impairs mitochondrial complex I in the rat liverKetaminaMitocondriaStress oxidativoFigadoMetabolismo EnergéticoGlicogênioKetamine can induce hepatotoxicity which has been suggested to be dependent on mitochondrial impairment. This study investigated the long-term effects of chronic low-dose ketamine on liver mitochondrial function, oxidative stress parameters, liver histology and glycogen content. Adult rats were administered with saline or ketamine (5 or 10 mg/kg) twice a day for a fourteen-day period in order to mimic chronic treatments. Effects between groups were compared ten days after the treatment had ended. Liver mitochondrial function was monitored in isolated mitochondrial extracts through evaluation of respiration parameters and activity of respiratory complexes, as well as oxidative stress, through lipid peroxidation, protein oxidation and superoxide dismutase activity. The hepatic histology and liver glycogen content were also evaluated. Ketamine groups showed a decreased evolution in body weight gains during the treatment period. Ketamine had no effect either on serum liver enzymes or on the oxidative stress parameters of liver mitochondria. Ketamine decreased the hepatic glycogen content, inhibited mitochondrial complex I and oxygen consumption when glutamate–malate substrate was used. These findings reflect a long-term mitochondrial bioenergetic deterioration induced by ketamine, which may explain the increased susceptibility of some patients to its prolonged or repeated use.ElsevierRepositório Científico do Instituto Politécnico do PortoVenâncio, CarlosAntunes, LuisFélix, LuísRodrigues, PaulaSummavielle, TeresaPeixoto, Francisco2018-11-26T15:48:48Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/12282engVenâncio, C., Antunes, L., Félix, L., Rodrigues, P., Summavielle, T., & Peixoto, F. (2013). Chronic ketamine administration impairs mitochondrial complex I in the rat liver. Life Sciences, 93(12), 464–470. https://doi.org/10.1016/j.lfs.2013.08.00110.1016/j.lfs.2013.08.001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:52:56Zoai:recipp.ipp.pt:10400.22/12282Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:27:12.014556Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
title |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
spellingShingle |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver Venâncio, Carlos Ketamina Mitocondria Stress oxidativo Figado Metabolismo Energético Glicogênio |
title_short |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
title_full |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
title_fullStr |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
title_full_unstemmed |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
title_sort |
Chronic ketamine administration impairs mitochondrial complex I in the rat liver |
author |
Venâncio, Carlos |
author_facet |
Venâncio, Carlos Antunes, Luis Félix, Luís Rodrigues, Paula Summavielle, Teresa Peixoto, Francisco |
author_role |
author |
author2 |
Antunes, Luis Félix, Luís Rodrigues, Paula Summavielle, Teresa Peixoto, Francisco |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Venâncio, Carlos Antunes, Luis Félix, Luís Rodrigues, Paula Summavielle, Teresa Peixoto, Francisco |
dc.subject.por.fl_str_mv |
Ketamina Mitocondria Stress oxidativo Figado Metabolismo Energético Glicogênio |
topic |
Ketamina Mitocondria Stress oxidativo Figado Metabolismo Energético Glicogênio |
description |
Ketamine can induce hepatotoxicity which has been suggested to be dependent on mitochondrial impairment. This study investigated the long-term effects of chronic low-dose ketamine on liver mitochondrial function, oxidative stress parameters, liver histology and glycogen content. Adult rats were administered with saline or ketamine (5 or 10 mg/kg) twice a day for a fourteen-day period in order to mimic chronic treatments. Effects between groups were compared ten days after the treatment had ended. Liver mitochondrial function was monitored in isolated mitochondrial extracts through evaluation of respiration parameters and activity of respiratory complexes, as well as oxidative stress, through lipid peroxidation, protein oxidation and superoxide dismutase activity. The hepatic histology and liver glycogen content were also evaluated. Ketamine groups showed a decreased evolution in body weight gains during the treatment period. Ketamine had no effect either on serum liver enzymes or on the oxidative stress parameters of liver mitochondria. Ketamine decreased the hepatic glycogen content, inhibited mitochondrial complex I and oxygen consumption when glutamate–malate substrate was used. These findings reflect a long-term mitochondrial bioenergetic deterioration induced by ketamine, which may explain the increased susceptibility of some patients to its prolonged or repeated use. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z 2018-11-26T15:48:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/12282 |
url |
http://hdl.handle.net/10400.22/12282 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Venâncio, C., Antunes, L., Félix, L., Rodrigues, P., Summavielle, T., & Peixoto, F. (2013). Chronic ketamine administration impairs mitochondrial complex I in the rat liver. Life Sciences, 93(12), 464–470. https://doi.org/10.1016/j.lfs.2013.08.001 10.1016/j.lfs.2013.08.001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131367289651200 |