Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix
| Autor(a) principal: | |
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| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/32910 |
Resumo: | Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
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Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervixAngiogenesisCervical carcinomaHypoxiaLymphangiogenesisMetabolic reprogrammingMonocarboxylate transporterVEGFScience & TechnologyLactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervixBACKGROUND: Tumour microenvironment is a fundamental aspect of tumour behaviour, modulating important events as cancer cell migration and invasion, as well as angiogenesis and metastisation. Among other microenvironment features, hypoxia and acidity play important roles in this modulation. As the metabolic reprogramming of cancer cells induces extracellular acidity, which in turn induces angiogenesis, and hypoxia induces both the metabolic reprogramming and angiogenesis, the present study aims to evaluate the immunohistochemical expression of a variety of metabolic and vascular markers as common targets of the hypoxic microenvironment in a series of cervical squamous cells carcinoma, as well as using an in vitro 3D culture model. METHODS: Immunohistochemical expression of MCT1, MCT4, CD147, GLUT1 and CAIX was assessed in a series of 28 chronic cervicitis, 34 LSIL, 29 HSIL, 38 cases of squamous cells carcinoma (SCC), as well as in in vitro 3D culture of keratinocytes expressing HPV genes. Furthermore, VEGF family members' expression was assessed in the SCC cases. The expression profiles were associated with patients' clinicopathological parameters. RESULTS: We found an increase of MCT4 expression along progression to malignancy in cervical samples. Also, MCT4 was associated with CD147 and CAIX expression. VEGF-A expression was more frequently found in cases without MCT1 expression. Both MCT4 and CD147 were more frequently expressed in younger patients at diagnosis while no associations were found between VEGF family and clinicopathological parameters. Finally, we show evidence for the upregulation of MCT4, as well as CD147 and CAIX, after HPV transfection. CONCLUSIONS: The results herein presented point at MCT4 as a promising therapeutic target in squamous cells carcinoma of the uterine cervix. Importantly, we show a possible association between lactate transport and angiogenesis, which should be further explored.CP received a post-doctoral fellowship (SFRH/BPD/69479/2010) and FM-S received a doctoral fellowship (SFRH/BD/87139/2012) from FCT (Portuguese Foundation for Science and Technology). This work was supported by the FCT grant ref. PTDC/SAU-FCF/104347/2008, under the scope of "Programa Operacional Tematico Factores de Competitividade" (COMPETE) of "Quadro Comunitario de Apoio III" and co-financed by Fundo Comunitario Europeu FEDER and also by FAPESP 2008/03232-1 to LLV.The Open Access PublisherUniversidade do MinhoPinheiro, CélineGarcia, Eduardo AnselmoSantos, Filipa MoraisNeto, Cristovam ScapulatempoMafra, AlliniSteenbergen, Renske DMBoccardo, EnriqueVilla, Luisa LinaBaltazar, FátimaLongatto Filho, Adhemar20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32910eng1471-240710.1186/1471-2407-14-75125296855http://www.biomedcentral.com/1471-2407/14/751info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:48Zoai:repositorium.sdum.uminho.pt:1822/32910Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:51.867001Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| title |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| spellingShingle |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix Pinheiro, Céline Angiogenesis Cervical carcinoma Hypoxia Lymphangiogenesis Metabolic reprogramming Monocarboxylate transporter VEGF Science & Technology |
| title_short |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| title_full |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| title_fullStr |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| title_full_unstemmed |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| title_sort |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| author |
Pinheiro, Céline |
| author_facet |
Pinheiro, Céline Garcia, Eduardo Anselmo Santos, Filipa Morais Neto, Cristovam Scapulatempo Mafra, Allini Steenbergen, Renske DM Boccardo, Enrique Villa, Luisa Lina Baltazar, Fátima Longatto Filho, Adhemar |
| author_role |
author |
| author2 |
Garcia, Eduardo Anselmo Santos, Filipa Morais Neto, Cristovam Scapulatempo Mafra, Allini Steenbergen, Renske DM Boccardo, Enrique Villa, Luisa Lina Baltazar, Fátima Longatto Filho, Adhemar |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Pinheiro, Céline Garcia, Eduardo Anselmo Santos, Filipa Morais Neto, Cristovam Scapulatempo Mafra, Allini Steenbergen, Renske DM Boccardo, Enrique Villa, Luisa Lina Baltazar, Fátima Longatto Filho, Adhemar |
| dc.subject.por.fl_str_mv |
Angiogenesis Cervical carcinoma Hypoxia Lymphangiogenesis Metabolic reprogramming Monocarboxylate transporter VEGF Science & Technology |
| topic |
Angiogenesis Cervical carcinoma Hypoxia Lymphangiogenesis Metabolic reprogramming Monocarboxylate transporter VEGF Science & Technology |
| description |
Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/32910 |
| url |
http://hdl.handle.net/1822/32910 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1471-2407 10.1186/1471-2407-14-751 25296855 http://www.biomedcentral.com/1471-2407/14/751 |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
The Open Access Publisher |
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The Open Access Publisher |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132379159199744 |