Ganoderma lucidum effects in an animal model of obesity

Detalhes bibliográficos
Autor(a) principal: Castro-Ribeiro, Carla
Data de Publicação: 2021
Outros Autores: Silva-Reis, Rita, Mendes-Gonçalves, Mariana, Ferreira, Rita, Taofiq, O, Barros, L, Martins, Tânia, Faustino-Rocha, Ana Isabel, Pires, Maria João, Pinto, Maria de Lurdes, Ferreira, Isabel CFR, Venâncio, Carlos, Rosa, Eduardo, Oliveira, Paula Alexandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/30679
Resumo: Obesity is a pandemic disease, and its evolution can be influenced by regular consumption of natural bioactive compounds. Mushrooms, such as Ganoderma lucidum (GL), include a wide variety of biomolecules with potential anti-obesity effects. This work aimed to study the influence of GL in an animal model of obesity. Forty-eight male mice (C57BL/6J) were divided into 5 groups: Group (G)-1-Western Control Diet; G-2-Western Diet 0.2% Cholesterol (WDC); G-3-WDC+0.7g/kg of GL; G-4-WDC+1.4g/ kg of GL; G-5 WDC+2.8g/kg of GL. Water and food consumption were recorded weekly. Animals were weighed individually, and their temperature was measured on the back and abdomen (thermographic camera FLIRE6390). Thirteen weeks later, animals were sacrificed, and organs collected. A liver portion was used for histopathology analysis (ethical approval no 8776). Chemical characterization of the GL extract was profiled by HPLC-DAD-ESI/MS. Ganoderic acid H and p-hydroxybenzoic acid were the main triterpenic and phenolic acids in the extract, respectively. Food and water consumption were no different among groups. All animals showed weight gain (11-16%): animals without GL supplementation showed the highest weight gain (G2 and G1, respectively); the groups with GL showed the lowest weight gain, which decreased as the mushroom concentration increased. Basal and final body temperatures remained constant. Liver weight was different among groups (p<0.05). Microvesicular (39/47; 83%) and macrovesicular fatty changes (steatosis) were the most frequent morphological changes in the liver. Hyperlipidemic diets consumption promoted weight gain and obesity. The higher weight gain in the groups not supplemented with GL suggested its anti-obesity properties. In this study, animals developed steatosis, which was not modified by GL supplementation. However, it should be noticed that the changes in lipid metabolism are reversible, and the differences observed in the liver relative weights, lower in the groups supplemented with GL, suggested an ongoing process of restoring to normal hepatocyte’s phenotype.
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spelling Ganoderma lucidum effects in an animal model of obesityObesity is a pandemic disease, and its evolution can be influenced by regular consumption of natural bioactive compounds. Mushrooms, such as Ganoderma lucidum (GL), include a wide variety of biomolecules with potential anti-obesity effects. This work aimed to study the influence of GL in an animal model of obesity. Forty-eight male mice (C57BL/6J) were divided into 5 groups: Group (G)-1-Western Control Diet; G-2-Western Diet 0.2% Cholesterol (WDC); G-3-WDC+0.7g/kg of GL; G-4-WDC+1.4g/ kg of GL; G-5 WDC+2.8g/kg of GL. Water and food consumption were recorded weekly. Animals were weighed individually, and their temperature was measured on the back and abdomen (thermographic camera FLIRE6390). Thirteen weeks later, animals were sacrificed, and organs collected. A liver portion was used for histopathology analysis (ethical approval no 8776). Chemical characterization of the GL extract was profiled by HPLC-DAD-ESI/MS. Ganoderic acid H and p-hydroxybenzoic acid were the main triterpenic and phenolic acids in the extract, respectively. Food and water consumption were no different among groups. All animals showed weight gain (11-16%): animals without GL supplementation showed the highest weight gain (G2 and G1, respectively); the groups with GL showed the lowest weight gain, which decreased as the mushroom concentration increased. Basal and final body temperatures remained constant. Liver weight was different among groups (p<0.05). Microvesicular (39/47; 83%) and macrovesicular fatty changes (steatosis) were the most frequent morphological changes in the liver. Hyperlipidemic diets consumption promoted weight gain and obesity. The higher weight gain in the groups not supplemented with GL suggested its anti-obesity properties. In this study, animals developed steatosis, which was not modified by GL supplementation. However, it should be noticed that the changes in lipid metabolism are reversible, and the differences observed in the liver relative weights, lower in the groups supplemented with GL, suggested an ongoing process of restoring to normal hepatocyte’s phenotype.2022-01-07T22:50:27Z2022-01-072021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/30679http://hdl.handle.net/10174/30679engCastro-Ribeiro C, Silva-Reis R, Mendes-Gonçalves M, Ferreira R, Taofiq O, Barros L, Martins T, Faustino-Rocha AI, Pires MJ, Pinto ML, Ferreira ICFR, Venâncio C, Rosa E, Oliveira PA. 2021. Ganoderma lucidum effects in an animal model of obesity. Webinar on Animal and Veterinary Science, p.16, 13 de junho.16ndndndndndndndanafaustino@uevora.ptndndndndndnd206Castro-Ribeiro, CarlaSilva-Reis, RitaMendes-Gonçalves, MarianaFerreira, RitaTaofiq, OBarros, LMartins, TâniaFaustino-Rocha, Ana IsabelPires, Maria JoãoPinto, Maria de LurdesFerreira, Isabel CFRVenâncio, CarlosRosa, EduardoOliveira, Paula Alexandrainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:27:40Zoai:dspace.uevora.pt:10174/30679Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:19:35.318582Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ganoderma lucidum effects in an animal model of obesity
title Ganoderma lucidum effects in an animal model of obesity
spellingShingle Ganoderma lucidum effects in an animal model of obesity
Castro-Ribeiro, Carla
title_short Ganoderma lucidum effects in an animal model of obesity
title_full Ganoderma lucidum effects in an animal model of obesity
title_fullStr Ganoderma lucidum effects in an animal model of obesity
title_full_unstemmed Ganoderma lucidum effects in an animal model of obesity
title_sort Ganoderma lucidum effects in an animal model of obesity
author Castro-Ribeiro, Carla
author_facet Castro-Ribeiro, Carla
Silva-Reis, Rita
Mendes-Gonçalves, Mariana
Ferreira, Rita
Taofiq, O
Barros, L
Martins, Tânia
Faustino-Rocha, Ana Isabel
Pires, Maria João
Pinto, Maria de Lurdes
Ferreira, Isabel CFR
Venâncio, Carlos
Rosa, Eduardo
Oliveira, Paula Alexandra
author_role author
author2 Silva-Reis, Rita
Mendes-Gonçalves, Mariana
Ferreira, Rita
Taofiq, O
Barros, L
Martins, Tânia
Faustino-Rocha, Ana Isabel
Pires, Maria João
Pinto, Maria de Lurdes
Ferreira, Isabel CFR
Venâncio, Carlos
Rosa, Eduardo
Oliveira, Paula Alexandra
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Castro-Ribeiro, Carla
Silva-Reis, Rita
Mendes-Gonçalves, Mariana
Ferreira, Rita
Taofiq, O
Barros, L
Martins, Tânia
Faustino-Rocha, Ana Isabel
Pires, Maria João
Pinto, Maria de Lurdes
Ferreira, Isabel CFR
Venâncio, Carlos
Rosa, Eduardo
Oliveira, Paula Alexandra
description Obesity is a pandemic disease, and its evolution can be influenced by regular consumption of natural bioactive compounds. Mushrooms, such as Ganoderma lucidum (GL), include a wide variety of biomolecules with potential anti-obesity effects. This work aimed to study the influence of GL in an animal model of obesity. Forty-eight male mice (C57BL/6J) were divided into 5 groups: Group (G)-1-Western Control Diet; G-2-Western Diet 0.2% Cholesterol (WDC); G-3-WDC+0.7g/kg of GL; G-4-WDC+1.4g/ kg of GL; G-5 WDC+2.8g/kg of GL. Water and food consumption were recorded weekly. Animals were weighed individually, and their temperature was measured on the back and abdomen (thermographic camera FLIRE6390). Thirteen weeks later, animals were sacrificed, and organs collected. A liver portion was used for histopathology analysis (ethical approval no 8776). Chemical characterization of the GL extract was profiled by HPLC-DAD-ESI/MS. Ganoderic acid H and p-hydroxybenzoic acid were the main triterpenic and phenolic acids in the extract, respectively. Food and water consumption were no different among groups. All animals showed weight gain (11-16%): animals without GL supplementation showed the highest weight gain (G2 and G1, respectively); the groups with GL showed the lowest weight gain, which decreased as the mushroom concentration increased. Basal and final body temperatures remained constant. Liver weight was different among groups (p<0.05). Microvesicular (39/47; 83%) and macrovesicular fatty changes (steatosis) were the most frequent morphological changes in the liver. Hyperlipidemic diets consumption promoted weight gain and obesity. The higher weight gain in the groups not supplemented with GL suggested its anti-obesity properties. In this study, animals developed steatosis, which was not modified by GL supplementation. However, it should be noticed that the changes in lipid metabolism are reversible, and the differences observed in the liver relative weights, lower in the groups supplemented with GL, suggested an ongoing process of restoring to normal hepatocyte’s phenotype.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01T00:00:00Z
2022-01-07T22:50:27Z
2022-01-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/30679
http://hdl.handle.net/10174/30679
url http://hdl.handle.net/10174/30679
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castro-Ribeiro C, Silva-Reis R, Mendes-Gonçalves M, Ferreira R, Taofiq O, Barros L, Martins T, Faustino-Rocha AI, Pires MJ, Pinto ML, Ferreira ICFR, Venâncio C, Rosa E, Oliveira PA. 2021. Ganoderma lucidum effects in an animal model of obesity. Webinar on Animal and Veterinary Science, p.16, 13 de junho.
16
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nd
nd
nd
nd
nd
nd
anafaustino@uevora.pt
nd
nd
nd
nd
nd
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