Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/26650 |
Resumo: | H. pylori drug-resistant strains and non-compliance to therapy are the major causes of H. pylori eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was to assess the ability of docosahexaenoic acid (DHA) to inhibit H. pylori growth both in vitro and in a mouse model. The effectiveness of standard therapy (ST) in combination with DHA on H. pylori eradication and recurrence prevention success was also investigated. The effects of DHA on H. pylori growth were analyzed in an in vitro dose-response study and n in vivo model. We analized the ability of H. pylori to colonize mice gastric mucosa following DHA, ST or a combination of both treatments. Our data demonstrate that DHA decreases H. pylori growth in vitro in a dose-dependent manner. Furthermore, DHA inhibits H. pylori gastric colonization in vivo as well as decreases mouse gastric mucosa inflammation. Addition of DHA to ST was also associated with lower H. pylori infection recurrence in the mouse model. In conclusion, DHA is an inhibitor of H. pylori growth and its ability to colonize mouse stomach. DHA treatment is also associated with a lower recurrence of H. pylori infection in combination with ST. These observations pave the way to consider DHA as an adjunct agent in H. pylori eradication treatment. |
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Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonizationH. pylori drug-resistant strains and non-compliance to therapy are the major causes of H. pylori eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was to assess the ability of docosahexaenoic acid (DHA) to inhibit H. pylori growth both in vitro and in a mouse model. The effectiveness of standard therapy (ST) in combination with DHA on H. pylori eradication and recurrence prevention success was also investigated. The effects of DHA on H. pylori growth were analyzed in an in vitro dose-response study and n in vivo model. We analized the ability of H. pylori to colonize mice gastric mucosa following DHA, ST or a combination of both treatments. Our data demonstrate that DHA decreases H. pylori growth in vitro in a dose-dependent manner. Furthermore, DHA inhibits H. pylori gastric colonization in vivo as well as decreases mouse gastric mucosa inflammation. Addition of DHA to ST was also associated with lower H. pylori infection recurrence in the mouse model. In conclusion, DHA is an inhibitor of H. pylori growth and its ability to colonize mouse stomach. DHA treatment is also associated with a lower recurrence of H. pylori infection in combination with ST. These observations pave the way to consider DHA as an adjunct agent in H. pylori eradication treatment.Public Library of Science2019-10-02T10:39:19Z2012-01-01T00:00:00Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/26650eng1932-620310.1371/journal.pone.0035072Correia, MartaMichel, ValérieMatos, António A.Carvalho, PatríciaOliveira, Maria J.Ferreira, Rui M.Dillies, Marie-AgnèsHuerre, MichelSeruca, RaquelFigueiredo, CeuMachado, Jose C.Touati, Elietteinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:51:38Zoai:ria.ua.pt:10773/26650Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:59:35.700174Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
title |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
spellingShingle |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization Correia, Marta |
title_short |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
title_full |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
title_fullStr |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
title_full_unstemmed |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
title_sort |
Docosahexaenoic acid inhibits Helicobacter pylori growth in vitro and mice gastric mucosa colonization |
author |
Correia, Marta |
author_facet |
Correia, Marta Michel, Valérie Matos, António A. Carvalho, Patrícia Oliveira, Maria J. Ferreira, Rui M. Dillies, Marie-Agnès Huerre, Michel Seruca, Raquel Figueiredo, Ceu Machado, Jose C. Touati, Eliette |
author_role |
author |
author2 |
Michel, Valérie Matos, António A. Carvalho, Patrícia Oliveira, Maria J. Ferreira, Rui M. Dillies, Marie-Agnès Huerre, Michel Seruca, Raquel Figueiredo, Ceu Machado, Jose C. Touati, Eliette |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Correia, Marta Michel, Valérie Matos, António A. Carvalho, Patrícia Oliveira, Maria J. Ferreira, Rui M. Dillies, Marie-Agnès Huerre, Michel Seruca, Raquel Figueiredo, Ceu Machado, Jose C. Touati, Eliette |
description |
H. pylori drug-resistant strains and non-compliance to therapy are the major causes of H. pylori eradication failure. For some bacterial species it has been demonstrated that fatty acids have a growth inhibitory effect. Our main aim was to assess the ability of docosahexaenoic acid (DHA) to inhibit H. pylori growth both in vitro and in a mouse model. The effectiveness of standard therapy (ST) in combination with DHA on H. pylori eradication and recurrence prevention success was also investigated. The effects of DHA on H. pylori growth were analyzed in an in vitro dose-response study and n in vivo model. We analized the ability of H. pylori to colonize mice gastric mucosa following DHA, ST or a combination of both treatments. Our data demonstrate that DHA decreases H. pylori growth in vitro in a dose-dependent manner. Furthermore, DHA inhibits H. pylori gastric colonization in vivo as well as decreases mouse gastric mucosa inflammation. Addition of DHA to ST was also associated with lower H. pylori infection recurrence in the mouse model. In conclusion, DHA is an inhibitor of H. pylori growth and its ability to colonize mouse stomach. DHA treatment is also associated with a lower recurrence of H. pylori infection in combination with ST. These observations pave the way to consider DHA as an adjunct agent in H. pylori eradication treatment. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01T00:00:00Z 2012 2019-10-02T10:39:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/26650 |
url |
http://hdl.handle.net/10773/26650 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 10.1371/journal.pone.0035072 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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