Chironomus riparius proteome responses to spinosad exposure

Detalhes bibliográficos
Autor(a) principal: Monteiro, Hugo R.
Data de Publicação: 2020
Outros Autores: Pestana, João L.T., Soares, A.M.V.M., Devreese, Bart, Lemos, Marco F.L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/6124
Resumo: Funding: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UIDB/04292/2020, CESAM: UIDP/50017/2020+UIDB/50017/2020, FCT/MCTES through national funds, the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020, and project PROTEOME (PTDC/AAG-MAA/1302/2014) co-financed by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Program of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, and IF/01420/2015, respectively).
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spelling Chironomus riparius proteome responses to spinosad exposureAquatic insectsEcotoxicoproteomicsHemoglobiniTRAQNeurotoxic insecticidesFunding: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UIDB/04292/2020, CESAM: UIDP/50017/2020+UIDB/50017/2020, FCT/MCTES through national funds, the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020, and project PROTEOME (PTDC/AAG-MAA/1302/2014) co-financed by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Program of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, and IF/01420/2015, respectively).The potential of proteome responses as early-warning indicators of insecticide exposure was evaluated using the non-biting midge Chironomus riparius (Meigen) as the model organism. Chironomus riparius larvae were exposed to environmentally relevant concentrations of the neurotoxic pesticide spinosad to uncover molecular events that may provide insights on the long-term individual and population level consequences. The iTRAQ labeling method was performed to quantify protein abundance changes between exposed and non-exposed organisms. Data analysis revealed a general dose-dependent decrease in the abundance of globin proteins as a result of spinosad exposure. Additionally, the downregulation of actin and a larval cuticle protein was also observed after spinosad exposure, which may be related to previously determined C. riparius life-history traits impairment and biochemical responses. Present results suggest that protein profile changes can be used as early warning biomarkers of pesticide exposure and may provide a better mechanistic interpretation of the toxic response of organisms, aiding in the assessment of the ecological effects of environmental contamination. This work also contributes to the understanding of the sublethal effects of insecticides in invertebrates and their molecular targets.MDPIIC-OnlineMonteiro, Hugo R.Pestana, João L.T.Soares, A.M.V.M.Devreese, BartLemos, Marco F.L.2021-08-20T13:47:56Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/6124engMonteiro HR, Pestana JLT, Soares AMVM, Devreese B, Lemos MFL. Chironomus riparius Proteome Responses to Spinosad Exposure. Toxics. 2020 Dec 11;8(4):117. doi: 10.3390/toxics8040117. PMID: 33322338; PMCID: PMC7768432.2305-630410.3390/toxics8040117info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:52:25Zoai:iconline.ipleiria.pt:10400.8/6124Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:26.498491Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chironomus riparius proteome responses to spinosad exposure
title Chironomus riparius proteome responses to spinosad exposure
spellingShingle Chironomus riparius proteome responses to spinosad exposure
Monteiro, Hugo R.
Aquatic insects
Ecotoxicoproteomics
Hemoglobin
iTRAQ
Neurotoxic insecticides
title_short Chironomus riparius proteome responses to spinosad exposure
title_full Chironomus riparius proteome responses to spinosad exposure
title_fullStr Chironomus riparius proteome responses to spinosad exposure
title_full_unstemmed Chironomus riparius proteome responses to spinosad exposure
title_sort Chironomus riparius proteome responses to spinosad exposure
author Monteiro, Hugo R.
author_facet Monteiro, Hugo R.
Pestana, João L.T.
Soares, A.M.V.M.
Devreese, Bart
Lemos, Marco F.L.
author_role author
author2 Pestana, João L.T.
Soares, A.M.V.M.
Devreese, Bart
Lemos, Marco F.L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Monteiro, Hugo R.
Pestana, João L.T.
Soares, A.M.V.M.
Devreese, Bart
Lemos, Marco F.L.
dc.subject.por.fl_str_mv Aquatic insects
Ecotoxicoproteomics
Hemoglobin
iTRAQ
Neurotoxic insecticides
topic Aquatic insects
Ecotoxicoproteomics
Hemoglobin
iTRAQ
Neurotoxic insecticides
description Funding: This study had the support of the Fundação para a Ciência e a Tecnologia (FCT) through MARE: UIDB/04292/2020, CESAM: UIDP/50017/2020+UIDB/50017/2020, FCT/MCTES through national funds, the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020, and project PROTEOME (PTDC/AAG-MAA/1302/2014) co-financed by COMPETE (POCI-01-0145-FEDER-016773). The project was also partially funded by the Integrated Program of SR&TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. Hugo R. Monteiro and João L.T. Pestana wish to acknowledge the financial support given by FCT (SFRH/BD/80988/2011, and IF/01420/2015, respectively).
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-08-20T13:47:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/6124
url http://hdl.handle.net/10400.8/6124
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro HR, Pestana JLT, Soares AMVM, Devreese B, Lemos MFL. Chironomus riparius Proteome Responses to Spinosad Exposure. Toxics. 2020 Dec 11;8(4):117. doi: 10.3390/toxics8040117. PMID: 33322338; PMCID: PMC7768432.
2305-6304
10.3390/toxics8040117
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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