Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/258 |
Resumo: | The objective of this study was to evaluate the significance of heterotrophic growth in nitrifying biofilm reactors fed only with ammonium as an energy source. The diversity, abundance and spatial distribution of nitrifying bacteria were studied using a combination of molecular tools and mathematical modeling, in two biofilm reactors operated with different hydraulic retention times. The composition and distribution of nitrifying consortia in biofilms were quantified by fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes combined with confocal laser scanning microscopy (CLSM) and digital image analysis. Autotrophic and heterotrophic biofilm fractions determined by FISH were compared to the output from a multispecies model that incorporates soluble microbial products (SMP) production/consumption. In reactor R1 (short retention time) nearly 100% of the total bacteria could be identified as either ammonia- or nitrite-oxidizing bacteria by quantitative FISH analyses, while in reactor R2 (long retention time) the identification rate was only 73%, with the rest probably consisting of heterotrophs. Mathematical simulations were performed to evaluate the influence of the hydraulic retention time (HRT), biofilm thickness, and substrate utilization associated SMP production on the growth of heterotrophic bacteria. The model predicts that low HRTs resulted in a lower availability of SMPs leading to purely autotrophic biofilms. These model predictions are consistent with experimental observations. At HRTs that are about an order of magnitude larger than the reciprocal of the net maximum growth rate the majority of the active biomass will grow suspended in the bulk phase rather than in the biofilm. |
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Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modelingTecnologia ambiental, Outras ciências da engenharia e tecnologiasEnvironmental technology, Other engineering and technologiesThe objective of this study was to evaluate the significance of heterotrophic growth in nitrifying biofilm reactors fed only with ammonium as an energy source. The diversity, abundance and spatial distribution of nitrifying bacteria were studied using a combination of molecular tools and mathematical modeling, in two biofilm reactors operated with different hydraulic retention times. The composition and distribution of nitrifying consortia in biofilms were quantified by fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes combined with confocal laser scanning microscopy (CLSM) and digital image analysis. Autotrophic and heterotrophic biofilm fractions determined by FISH were compared to the output from a multispecies model that incorporates soluble microbial products (SMP) production/consumption. In reactor R1 (short retention time) nearly 100% of the total bacteria could be identified as either ammonia- or nitrite-oxidizing bacteria by quantitative FISH analyses, while in reactor R2 (long retention time) the identification rate was only 73%, with the rest probably consisting of heterotrophs. Mathematical simulations were performed to evaluate the influence of the hydraulic retention time (HRT), biofilm thickness, and substrate utilization associated SMP production on the growth of heterotrophic bacteria. The model predicts that low HRTs resulted in a lower availability of SMPs leading to purely autotrophic biofilms. These model predictions are consistent with experimental observations. At HRTs that are about an order of magnitude larger than the reciprocal of the net maximum growth rate the majority of the active biomass will grow suspended in the bulk phase rather than in the biofilm.20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/258eng0273-1223R. NogueiraD. ElenterA. BritoL. F. MeloM. WagnerE. Morgenrothinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:00:47Zoai:repositorio-aberto.up.pt:10216/258Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:13:38.816216Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
title |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
spellingShingle |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling R. Nogueira Tecnologia ambiental, Outras ciências da engenharia e tecnologias Environmental technology, Other engineering and technologies |
title_short |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
title_full |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
title_fullStr |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
title_full_unstemmed |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
title_sort |
Evaluating heterotrophic growth in a nitrifying biofilm reactor using fluorescence in situ hybridization and mathematical modeling |
author |
R. Nogueira |
author_facet |
R. Nogueira D. Elenter A. Brito L. F. Melo M. Wagner E. Morgenroth |
author_role |
author |
author2 |
D. Elenter A. Brito L. F. Melo M. Wagner E. Morgenroth |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
R. Nogueira D. Elenter A. Brito L. F. Melo M. Wagner E. Morgenroth |
dc.subject.por.fl_str_mv |
Tecnologia ambiental, Outras ciências da engenharia e tecnologias Environmental technology, Other engineering and technologies |
topic |
Tecnologia ambiental, Outras ciências da engenharia e tecnologias Environmental technology, Other engineering and technologies |
description |
The objective of this study was to evaluate the significance of heterotrophic growth in nitrifying biofilm reactors fed only with ammonium as an energy source. The diversity, abundance and spatial distribution of nitrifying bacteria were studied using a combination of molecular tools and mathematical modeling, in two biofilm reactors operated with different hydraulic retention times. The composition and distribution of nitrifying consortia in biofilms were quantified by fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes combined with confocal laser scanning microscopy (CLSM) and digital image analysis. Autotrophic and heterotrophic biofilm fractions determined by FISH were compared to the output from a multispecies model that incorporates soluble microbial products (SMP) production/consumption. In reactor R1 (short retention time) nearly 100% of the total bacteria could be identified as either ammonia- or nitrite-oxidizing bacteria by quantitative FISH analyses, while in reactor R2 (long retention time) the identification rate was only 73%, with the rest probably consisting of heterotrophs. Mathematical simulations were performed to evaluate the influence of the hydraulic retention time (HRT), biofilm thickness, and substrate utilization associated SMP production on the growth of heterotrophic bacteria. The model predicts that low HRTs resulted in a lower availability of SMPs leading to purely autotrophic biofilms. These model predictions are consistent with experimental observations. At HRTs that are about an order of magnitude larger than the reciprocal of the net maximum growth rate the majority of the active biomass will grow suspended in the bulk phase rather than in the biofilm. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2005-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/258 |
url |
https://hdl.handle.net/10216/258 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0273-1223 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136058354434049 |