Detection of a new variant of Citrus tristeza virus in Greek citrus crops
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/6234 |
Resumo: | Citrus tristeza virus (CTV), the most destructive virus of citrus, is a quarantine pathogen in Greece. Since 2000, several accidental imports of infected propagation material have been detected in the country, and while eradication measures were applied, a few disease foci still remain. CTV isolates were collected from Chania (Crete) and the "lemonwood" of Poros (Peloponnese), and their genetic variability was studied using single-strand conformation polymorphism (SSCP). One previously characterized isolate from Argolida grafted on a Mexican lime (GR3) and two Italian isolates from Calamondin were also included in the study. ELISA and RT-PCR tests confirmed CTV presence, and SSCP analysis of the virus amplified coat protein (CP) gene was used to separate either distinct virus isolates for cloning the CP gene or variants (haplotypes) for sequencing. Analyses showed that selected variants of four representative isolates clustered into three of the seven defined phylogenetic groups: groups 3b and 5 (severe isolates) and group M (mild isolates). The prevalent haplotypes detected in the CTV from lemonwood of Poros (GR9) were in group 3b, confirming previous results. However, one sequence variant was identified as a recombinant between haplotypes from groups 3b and 5. Variants of these two groups were also detected in the Italian Calamondin isolate. In the grafted Mexican lime isolate (GR3) from Argolida, only one haplotype was found which belonged to group M, while in the field isolate from Chania (GR6) the only haplotype detected was in group 5. This is the first report of variants of group 5 in Greece, suggesting an unknown virus introduction. The prevalence of severe isolates in the area is of particular concern, and implications for the future of the CTV epidemics are discussed. |
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Detection of a new variant of Citrus tristeza virus in Greek citrus cropsCitrus tristeza virus (CTV), the most destructive virus of citrus, is a quarantine pathogen in Greece. Since 2000, several accidental imports of infected propagation material have been detected in the country, and while eradication measures were applied, a few disease foci still remain. CTV isolates were collected from Chania (Crete) and the "lemonwood" of Poros (Peloponnese), and their genetic variability was studied using single-strand conformation polymorphism (SSCP). One previously characterized isolate from Argolida grafted on a Mexican lime (GR3) and two Italian isolates from Calamondin were also included in the study. ELISA and RT-PCR tests confirmed CTV presence, and SSCP analysis of the virus amplified coat protein (CP) gene was used to separate either distinct virus isolates for cloning the CP gene or variants (haplotypes) for sequencing. Analyses showed that selected variants of four representative isolates clustered into three of the seven defined phylogenetic groups: groups 3b and 5 (severe isolates) and group M (mild isolates). The prevalent haplotypes detected in the CTV from lemonwood of Poros (GR9) were in group 3b, confirming previous results. However, one sequence variant was identified as a recombinant between haplotypes from groups 3b and 5. Variants of these two groups were also detected in the Italian Calamondin isolate. In the grafted Mexican lime isolate (GR3) from Argolida, only one haplotype was found which belonged to group M, while in the field isolate from Chania (GR6) the only haplotype detected was in group 5. This is the first report of variants of group 5 in Greece, suggesting an unknown virus introduction. The prevalence of severe isolates in the area is of particular concern, and implications for the future of the CTV epidemics are discussed.Mediterranean Phytopathological UnionSapientiaChatzivassiliou, E. K.Nolasco, Gustavo2015-06-15T14:13:46Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/6234eng0031-9465AUT: GNO00324;https://dx.doi.org/10.14601/Phytopathol_Mediterr-12910info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:17:26Zoai:sapientia.ualg.pt:10400.1/6234Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:59:02.732570Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
title |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
spellingShingle |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops Chatzivassiliou, E. K. |
title_short |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
title_full |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
title_fullStr |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
title_full_unstemmed |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
title_sort |
Detection of a new variant of Citrus tristeza virus in Greek citrus crops |
author |
Chatzivassiliou, E. K. |
author_facet |
Chatzivassiliou, E. K. Nolasco, Gustavo |
author_role |
author |
author2 |
Nolasco, Gustavo |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Chatzivassiliou, E. K. Nolasco, Gustavo |
description |
Citrus tristeza virus (CTV), the most destructive virus of citrus, is a quarantine pathogen in Greece. Since 2000, several accidental imports of infected propagation material have been detected in the country, and while eradication measures were applied, a few disease foci still remain. CTV isolates were collected from Chania (Crete) and the "lemonwood" of Poros (Peloponnese), and their genetic variability was studied using single-strand conformation polymorphism (SSCP). One previously characterized isolate from Argolida grafted on a Mexican lime (GR3) and two Italian isolates from Calamondin were also included in the study. ELISA and RT-PCR tests confirmed CTV presence, and SSCP analysis of the virus amplified coat protein (CP) gene was used to separate either distinct virus isolates for cloning the CP gene or variants (haplotypes) for sequencing. Analyses showed that selected variants of four representative isolates clustered into three of the seven defined phylogenetic groups: groups 3b and 5 (severe isolates) and group M (mild isolates). The prevalent haplotypes detected in the CTV from lemonwood of Poros (GR9) were in group 3b, confirming previous results. However, one sequence variant was identified as a recombinant between haplotypes from groups 3b and 5. Variants of these two groups were also detected in the Italian Calamondin isolate. In the grafted Mexican lime isolate (GR3) from Argolida, only one haplotype was found which belonged to group M, while in the field isolate from Chania (GR6) the only haplotype detected was in group 5. This is the first report of variants of group 5 in Greece, suggesting an unknown virus introduction. The prevalence of severe isolates in the area is of particular concern, and implications for the future of the CTV epidemics are discussed. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z 2015-06-15T14:13:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/6234 |
url |
http://hdl.handle.net/10400.1/6234 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0031-9465 AUT: GNO00324; https://dx.doi.org/10.14601/Phytopathol_Mediterr-12910 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mediterranean Phytopathological Union |
publisher.none.fl_str_mv |
Mediterranean Phytopathological Union |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133210587693056 |