Effect of ionizing radiation on rat peritoneal macrophages

Detalhes bibliográficos
Autor(a) principal: Cunha, Ana
Data de Publicação: 2007
Outros Autores: Lourenço, António, Cancela, João, Castelhano, João, Carvalho, Sara, Saiote, Ana, Matias, Áurea, Reis, Emília, Lopes, Carmo, Santos, Ana, Botelho, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://proa.ua.pt/index.php/revdeti/article/view/17169
Resumo: This study aims to evaluate the survival rate of peritoneal rat macrophages after exposure to 3 & 6 Gy ionizing radiation fields, respectively. Cells were cultured in multiwell plates (37 ºC, 5% CO2), separated in 3 groups: GI–control; GII-cells irradiated with 3 Gy; GIII–cells irradiated with 6 Gy, using a 4 MeV particle accelerator. After irradiation cells were incubated for 2h30 = T0, 13 h = T1, 20 h = T2, 36 h = T3, 70 h = T4, 90 h = T5 post-irradiation. To evaluate the cell’s viability the colorimetric MTT test has been used and colour intensity was measured by micro ELISA. Visual control was done with a contrast phase optical microscope. Irradiation with 6 Gy is the most harmful for these cells, showing death of the majority of them. 3 Gy is also aggressive but enables a higher survival rate. Time is also an important factor to which cell death is proportional. Our results show a decrease of living cells with time and higher doses.
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spelling Effect of ionizing radiation on rat peritoneal macrophagesThis study aims to evaluate the survival rate of peritoneal rat macrophages after exposure to 3 & 6 Gy ionizing radiation fields, respectively. Cells were cultured in multiwell plates (37 ºC, 5% CO2), separated in 3 groups: GI–control; GII-cells irradiated with 3 Gy; GIII–cells irradiated with 6 Gy, using a 4 MeV particle accelerator. After irradiation cells were incubated for 2h30 = T0, 13 h = T1, 20 h = T2, 36 h = T3, 70 h = T4, 90 h = T5 post-irradiation. To evaluate the cell’s viability the colorimetric MTT test has been used and colour intensity was measured by micro ELISA. Visual control was done with a contrast phase optical microscope. Irradiation with 6 Gy is the most harmful for these cells, showing death of the majority of them. 3 Gy is also aggressive but enables a higher survival rate. Time is also an important factor to which cell death is proportional. Our results show a decrease of living cells with time and higher doses.UA Editora2007-01-01T00:00:00Zconference objectconference objectinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://proa.ua.pt/index.php/revdeti/article/view/17169oai:proa.ua.pt:article/17169Eletrónica e Telecomunicações; Vol 4 No 7 (2007); 818-821Eletrónica e Telecomunicações; vol. 4 n.º 7 (2007); 818-8212182-97721645-0493reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://proa.ua.pt/index.php/revdeti/article/view/17169https://proa.ua.pt/index.php/revdeti/article/view/17169/12222https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessCunha, AnaLourenço, AntónioCancela, JoãoCastelhano, JoãoCarvalho, SaraSaiote, AnaMatias, ÁureaReis, EmíliaLopes, CarmoSantos, AnaBotelho, Filomena2022-09-26T11:00:11Zoai:proa.ua.pt:article/17169Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:08:07.851824Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of ionizing radiation on rat peritoneal macrophages
title Effect of ionizing radiation on rat peritoneal macrophages
spellingShingle Effect of ionizing radiation on rat peritoneal macrophages
Cunha, Ana
title_short Effect of ionizing radiation on rat peritoneal macrophages
title_full Effect of ionizing radiation on rat peritoneal macrophages
title_fullStr Effect of ionizing radiation on rat peritoneal macrophages
title_full_unstemmed Effect of ionizing radiation on rat peritoneal macrophages
title_sort Effect of ionizing radiation on rat peritoneal macrophages
author Cunha, Ana
author_facet Cunha, Ana
Lourenço, António
Cancela, João
Castelhano, João
Carvalho, Sara
Saiote, Ana
Matias, Áurea
Reis, Emília
Lopes, Carmo
Santos, Ana
Botelho, Filomena
author_role author
author2 Lourenço, António
Cancela, João
Castelhano, João
Carvalho, Sara
Saiote, Ana
Matias, Áurea
Reis, Emília
Lopes, Carmo
Santos, Ana
Botelho, Filomena
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Ana
Lourenço, António
Cancela, João
Castelhano, João
Carvalho, Sara
Saiote, Ana
Matias, Áurea
Reis, Emília
Lopes, Carmo
Santos, Ana
Botelho, Filomena
description This study aims to evaluate the survival rate of peritoneal rat macrophages after exposure to 3 & 6 Gy ionizing radiation fields, respectively. Cells were cultured in multiwell plates (37 ºC, 5% CO2), separated in 3 groups: GI–control; GII-cells irradiated with 3 Gy; GIII–cells irradiated with 6 Gy, using a 4 MeV particle accelerator. After irradiation cells were incubated for 2h30 = T0, 13 h = T1, 20 h = T2, 36 h = T3, 70 h = T4, 90 h = T5 post-irradiation. To evaluate the cell’s viability the colorimetric MTT test has been used and colour intensity was measured by micro ELISA. Visual control was done with a contrast phase optical microscope. Irradiation with 6 Gy is the most harmful for these cells, showing death of the majority of them. 3 Gy is also aggressive but enables a higher survival rate. Time is also an important factor to which cell death is proportional. Our results show a decrease of living cells with time and higher doses.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01T00:00:00Z
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url https://proa.ua.pt/index.php/revdeti/article/view/17169
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dc.language.iso.fl_str_mv eng
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https://proa.ua.pt/index.php/revdeti/article/view/17169/12222
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dc.publisher.none.fl_str_mv UA Editora
publisher.none.fl_str_mv UA Editora
dc.source.none.fl_str_mv Eletrónica e Telecomunicações; Vol 4 No 7 (2007); 818-821
Eletrónica e Telecomunicações; vol. 4 n.º 7 (2007); 818-821
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