Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/5933 |
Resumo: | Borrelia burgdorferi sensu lato comprises a species complex of tick-transmitted bacteria that includes the agents of human Lyme borreliosis. Borrelia turdi is a genospecies of this complex that exists in cryptic transmission cycles mainly between ornithophilic tick vectors and their avian hosts. The species has been originally discovered in avian transmission cycles in Asia but has increasingly been found in Europe. Next generation sequencing was used to sequence the genome of B. turdi isolates obtained from ticks feeding on birds in Portugal to better understand the evolution and phylogenetic relationship of this avian and ornithophilic tick-associated genospecies. Here we use draft genomes of these B. turdi isolates for comparative analysis and to determine the taxonomic position within the B. burgdorferi s.l. species complex. The main chromosomes showed a maximum similarity of 93% to other Borrelia species whilst most plasmids had lower similarities. All three isolates had nine or 10 plasmids and, interestingly, one plasmid with a novel partitioning protein; this plasmid was termed lp30. Phylogenetic analysis of multilocus sequence typing housekeeping genes and 113 single copy orthologous genes revealed that the isolates clustered according to their classification as B. turdi. In phylogenies generated from these 113 genes the isolates cluster together with other Eurasian genospecies and form a sister clade to the avian associated B. garinii and the rodent associated B. bavariensis. These findings show that Borrelia species maintained in cryptic ecological cycles need to be included to fully understand the complex ecology and evolutionary history of this bacterial species complex. |
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Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia gariniiBirdBorrelia burgdorferi sensu latoBorrelia turdiHost AssociationIlluminaSPAdesGenome AssemblyInfecções Sistémicas e ZoonosesBorrelia burgdorferi sensu lato comprises a species complex of tick-transmitted bacteria that includes the agents of human Lyme borreliosis. Borrelia turdi is a genospecies of this complex that exists in cryptic transmission cycles mainly between ornithophilic tick vectors and their avian hosts. The species has been originally discovered in avian transmission cycles in Asia but has increasingly been found in Europe. Next generation sequencing was used to sequence the genome of B. turdi isolates obtained from ticks feeding on birds in Portugal to better understand the evolution and phylogenetic relationship of this avian and ornithophilic tick-associated genospecies. Here we use draft genomes of these B. turdi isolates for comparative analysis and to determine the taxonomic position within the B. burgdorferi s.l. species complex. The main chromosomes showed a maximum similarity of 93% to other Borrelia species whilst most plasmids had lower similarities. All three isolates had nine or 10 plasmids and, interestingly, one plasmid with a novel partitioning protein; this plasmid was termed lp30. Phylogenetic analysis of multilocus sequence typing housekeeping genes and 113 single copy orthologous genes revealed that the isolates clustered according to their classification as B. turdi. In phylogenies generated from these 113 genes the isolates cluster together with other Eurasian genospecies and form a sister clade to the avian associated B. garinii and the rodent associated B. bavariensis. These findings show that Borrelia species maintained in cryptic ecological cycles need to be included to fully understand the complex ecology and evolutionary history of this bacterial species complex.This work was supported by the Robert-Koch-Institute through funding of the National Reference Center for Borrelia, by Fundação para a Ciência e a Tecnologia - FCT (MARE – UID/MAR/04292/2013; SFRH/BPD/108197/2015) and by the Portuguese National Institute of Health.Elsevier/ Academic PressRepositório Científico do Instituto Nacional de SaúdeMargos, G.Becker, N.S.Fingerle, V.Sing, A.Ramos, J.A.Lopes de Carvalho, I.Norte, A.C.2019-02-22T17:57:23Z2018-11-102018-11-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/5933engMol Phylogenet Evol. 2019 Feb;131:93-98. doi: 10.1016/j.ympev.2018.10.044. Epub 2018 Nov 101055-790310.1016/j.ympev.2018.10.044info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:09Zoai:repositorio.insa.pt:10400.18/5933Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:40.521647Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
title |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
spellingShingle |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii Margos, G. Bird Borrelia burgdorferi sensu lato Borrelia turdi Host Association Illumina SPAdes Genome Assembly Infecções Sistémicas e Zoonoses |
title_short |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
title_full |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
title_fullStr |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
title_full_unstemmed |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
title_sort |
Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii |
author |
Margos, G. |
author_facet |
Margos, G. Becker, N.S. Fingerle, V. Sing, A. Ramos, J.A. Lopes de Carvalho, I. Norte, A.C. |
author_role |
author |
author2 |
Becker, N.S. Fingerle, V. Sing, A. Ramos, J.A. Lopes de Carvalho, I. Norte, A.C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Margos, G. Becker, N.S. Fingerle, V. Sing, A. Ramos, J.A. Lopes de Carvalho, I. Norte, A.C. |
dc.subject.por.fl_str_mv |
Bird Borrelia burgdorferi sensu lato Borrelia turdi Host Association Illumina SPAdes Genome Assembly Infecções Sistémicas e Zoonoses |
topic |
Bird Borrelia burgdorferi sensu lato Borrelia turdi Host Association Illumina SPAdes Genome Assembly Infecções Sistémicas e Zoonoses |
description |
Borrelia burgdorferi sensu lato comprises a species complex of tick-transmitted bacteria that includes the agents of human Lyme borreliosis. Borrelia turdi is a genospecies of this complex that exists in cryptic transmission cycles mainly between ornithophilic tick vectors and their avian hosts. The species has been originally discovered in avian transmission cycles in Asia but has increasingly been found in Europe. Next generation sequencing was used to sequence the genome of B. turdi isolates obtained from ticks feeding on birds in Portugal to better understand the evolution and phylogenetic relationship of this avian and ornithophilic tick-associated genospecies. Here we use draft genomes of these B. turdi isolates for comparative analysis and to determine the taxonomic position within the B. burgdorferi s.l. species complex. The main chromosomes showed a maximum similarity of 93% to other Borrelia species whilst most plasmids had lower similarities. All three isolates had nine or 10 plasmids and, interestingly, one plasmid with a novel partitioning protein; this plasmid was termed lp30. Phylogenetic analysis of multilocus sequence typing housekeeping genes and 113 single copy orthologous genes revealed that the isolates clustered according to their classification as B. turdi. In phylogenies generated from these 113 genes the isolates cluster together with other Eurasian genospecies and form a sister clade to the avian associated B. garinii and the rodent associated B. bavariensis. These findings show that Borrelia species maintained in cryptic ecological cycles need to be included to fully understand the complex ecology and evolutionary history of this bacterial species complex. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-10 2018-11-10T00:00:00Z 2019-02-22T17:57:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/5933 |
url |
http://hdl.handle.net/10400.18/5933 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mol Phylogenet Evol. 2019 Feb;131:93-98. doi: 10.1016/j.ympev.2018.10.044. Epub 2018 Nov 10 1055-7903 10.1016/j.ympev.2018.10.044 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier/ Academic Press |
publisher.none.fl_str_mv |
Elsevier/ Academic Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132149474918400 |