Multi-enzyme systems in flow chemistry
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10437/12144 |
Resumo: | Recent years have witnessed a growing interest in the use of biocatalysts in flow reactors. This merging combines the high selectivity and mild operation conditions typical of biocatalysis with enhanced mass transfer and resource efficiency associated to flow chemistry. Additionally, it provides a sound environment to emulate Nature by mimicking metabolic pathways in living cells and to produce goods through the systematic organization of enzymes towards efficient cascade reactions. Moreover, by enabling the combination of enzymes from different hosts, this approach paves the way for novel pathways. The present review aims to present recent developments within the scope of flow chemistry involving multi-enzymatic cascade reactions. The types of reactors used are briefly addressed. Immobilization methodologies and strategies for the application of the immobilized biocatalysts are presented and discussed. Key aspects related to the use of whole cells in flow chemistry are presented. The combination of chemocatalysis and biocatalysis is also addressed and relevant aspects are highlighted. Challenges faced in the transition from microscale to industrial scale are presented and discussed |
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Multi-enzyme systems in flow chemistryBIOTECNOLOGIABIOQUÍMICAENZIMASMETABOLISMO CELULARREAÇÕES QUÍMICASBIOTHECNOLOGYBIOCHEMISTRYENZYMESCELLULAR METABOLISMCHEMICAL REACTIONSRecent years have witnessed a growing interest in the use of biocatalysts in flow reactors. This merging combines the high selectivity and mild operation conditions typical of biocatalysis with enhanced mass transfer and resource efficiency associated to flow chemistry. Additionally, it provides a sound environment to emulate Nature by mimicking metabolic pathways in living cells and to produce goods through the systematic organization of enzymes towards efficient cascade reactions. Moreover, by enabling the combination of enzymes from different hosts, this approach paves the way for novel pathways. The present review aims to present recent developments within the scope of flow chemistry involving multi-enzymatic cascade reactions. The types of reactors used are briefly addressed. Immobilization methodologies and strategies for the application of the immobilized biocatalysts are presented and discussed. Key aspects related to the use of whole cells in flow chemistry are presented. The combination of chemocatalysis and biocatalysis is also addressed and relevant aspects are highlighted. Challenges faced in the transition from microscale to industrial scale are presented and discussedMDPI2021-09-06T10:56:00Z2021-01-01T00:00:00Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10437/12144eng10.3390/pr9020225Fernandes, Pedro Carlos de BarrosCarvalho, Carla C. C. R. deinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:06:13Zoai:recil.ensinolusofona.pt:10437/12144Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:13:48.077306Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multi-enzyme systems in flow chemistry |
title |
Multi-enzyme systems in flow chemistry |
spellingShingle |
Multi-enzyme systems in flow chemistry Fernandes, Pedro Carlos de Barros BIOTECNOLOGIA BIOQUÍMICA ENZIMAS METABOLISMO CELULAR REAÇÕES QUÍMICAS BIOTHECNOLOGY BIOCHEMISTRY ENZYMES CELLULAR METABOLISM CHEMICAL REACTIONS |
title_short |
Multi-enzyme systems in flow chemistry |
title_full |
Multi-enzyme systems in flow chemistry |
title_fullStr |
Multi-enzyme systems in flow chemistry |
title_full_unstemmed |
Multi-enzyme systems in flow chemistry |
title_sort |
Multi-enzyme systems in flow chemistry |
author |
Fernandes, Pedro Carlos de Barros |
author_facet |
Fernandes, Pedro Carlos de Barros Carvalho, Carla C. C. R. de |
author_role |
author |
author2 |
Carvalho, Carla C. C. R. de |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Fernandes, Pedro Carlos de Barros Carvalho, Carla C. C. R. de |
dc.subject.por.fl_str_mv |
BIOTECNOLOGIA BIOQUÍMICA ENZIMAS METABOLISMO CELULAR REAÇÕES QUÍMICAS BIOTHECNOLOGY BIOCHEMISTRY ENZYMES CELLULAR METABOLISM CHEMICAL REACTIONS |
topic |
BIOTECNOLOGIA BIOQUÍMICA ENZIMAS METABOLISMO CELULAR REAÇÕES QUÍMICAS BIOTHECNOLOGY BIOCHEMISTRY ENZYMES CELLULAR METABOLISM CHEMICAL REACTIONS |
description |
Recent years have witnessed a growing interest in the use of biocatalysts in flow reactors. This merging combines the high selectivity and mild operation conditions typical of biocatalysis with enhanced mass transfer and resource efficiency associated to flow chemistry. Additionally, it provides a sound environment to emulate Nature by mimicking metabolic pathways in living cells and to produce goods through the systematic organization of enzymes towards efficient cascade reactions. Moreover, by enabling the combination of enzymes from different hosts, this approach paves the way for novel pathways. The present review aims to present recent developments within the scope of flow chemistry involving multi-enzymatic cascade reactions. The types of reactors used are briefly addressed. Immobilization methodologies and strategies for the application of the immobilized biocatalysts are presented and discussed. Key aspects related to the use of whole cells in flow chemistry are presented. The combination of chemocatalysis and biocatalysis is also addressed and relevant aspects are highlighted. Challenges faced in the transition from microscale to industrial scale are presented and discussed |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-06T10:56:00Z 2021-01-01T00:00:00Z 2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10437/12144 |
url |
http://hdl.handle.net/10437/12144 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/pr9020225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131234978234368 |