Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/102833 https://doi.org/10.3390/agronomy12051082 |
Resumo: | Plants propagated by seed do not ensure genetic uniformity and are sometimes infected with diseases. In Vitro micropropagation techniques are an alternative method to traditional cloning approaches for producing true-to-type and pathogen-free plants. However, due to the particularities of the in vitro environment, these plants face many challenges, often critical to their survival, to adapt to ex vitro conditions. In this context, four substrates and two types of mineral nutrition (quick-release (QRF) and controlled-release (CRF) fertilizers), as well as their absence were evaluated in the process of acclimatization of Solanum betaceum plants. Stomatal conductance (gs), chlorophyll content index (CCI), and dry biomass of roots, shoots, and entire plants were the parameters analyzed during the acclimatization. The best crop performance (gs, CCI, and dry biomass) were observed in substrates consisting of vermiculite plus the application of mineral nutrients through a CRF, proving that mineral nutrition has the greatest positive impact on the acclimatization process. In these conditions, plants were obtained with a total dry biomass being significantly higher (515.0 mg (QRF) and 635.9 mg (CRF) when compared to the total dry biomass of untreated plants (119.9 mg). The best conditions for this first experiment were replicated in a second test in order to assess the best fertilizer amount suited for plantlet growth. In this case, the best results were obtained with 0.4 g of CRF, in which plants showed a dry biomass of roots (542.7 mg) and a total dry biomass (594.5 mg), which was significantly higher than in the control (183.2 mg and 165.9 mg, respectively) or with other concentrations of CRF (0.8 and 1.6 g). A similar trend was found concerning the CCI (5.3) and gs (72.5 mmol m2 s1) in which 0.4 g CRF gave also the best results when compared with the control (without CRF) or with 0.8 g (4.7 and 56.2 mmol m2 s1) and 1.6 g (4.7 and 52.2 mmol m2 s1) treatments. In general, it was found that tamarillo plantlets acclimatized to 0.4 g of CRF had a faster initial growth and better performance (CCI and gs), with plants ready to go to the greenhouse/field more quickly, thus reducing the time to obtain suitable plants for the market and shortening the production cycle. |
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Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutritionchlorophyll contentcontrolled-release fertilizerdry biomassin vitro micropropagationquick-release fertilizerPlants propagated by seed do not ensure genetic uniformity and are sometimes infected with diseases. In Vitro micropropagation techniques are an alternative method to traditional cloning approaches for producing true-to-type and pathogen-free plants. However, due to the particularities of the in vitro environment, these plants face many challenges, often critical to their survival, to adapt to ex vitro conditions. In this context, four substrates and two types of mineral nutrition (quick-release (QRF) and controlled-release (CRF) fertilizers), as well as their absence were evaluated in the process of acclimatization of Solanum betaceum plants. Stomatal conductance (gs), chlorophyll content index (CCI), and dry biomass of roots, shoots, and entire plants were the parameters analyzed during the acclimatization. The best crop performance (gs, CCI, and dry biomass) were observed in substrates consisting of vermiculite plus the application of mineral nutrients through a CRF, proving that mineral nutrition has the greatest positive impact on the acclimatization process. In these conditions, plants were obtained with a total dry biomass being significantly higher (515.0 mg (QRF) and 635.9 mg (CRF) when compared to the total dry biomass of untreated plants (119.9 mg). The best conditions for this first experiment were replicated in a second test in order to assess the best fertilizer amount suited for plantlet growth. In this case, the best results were obtained with 0.4 g of CRF, in which plants showed a dry biomass of roots (542.7 mg) and a total dry biomass (594.5 mg), which was significantly higher than in the control (183.2 mg and 165.9 mg, respectively) or with other concentrations of CRF (0.8 and 1.6 g). A similar trend was found concerning the CCI (5.3) and gs (72.5 mmol m2 s1) in which 0.4 g CRF gave also the best results when compared with the control (without CRF) or with 0.8 g (4.7 and 56.2 mmol m2 s1) and 1.6 g (4.7 and 52.2 mmol m2 s1) treatments. In general, it was found that tamarillo plantlets acclimatized to 0.4 g of CRF had a faster initial growth and better performance (CCI and gs), with plants ready to go to the greenhouse/field more quickly, thus reducing the time to obtain suitable plants for the market and shortening the production cycle.2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/102833http://hdl.handle.net/10316/102833https://doi.org/10.3390/agronomy12051082eng2073-4395Pirata, Madalena SalgadoCorreia, SandraCanhoto, Jorgeinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-18T20:31:45Zoai:estudogeral.uc.pt:10316/102833Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:19:44.741001Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
title |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
spellingShingle |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition Pirata, Madalena Salgado chlorophyll content controlled-release fertilizer dry biomass in vitro micropropagation quick-release fertilizer |
title_short |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
title_full |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
title_fullStr |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
title_full_unstemmed |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
title_sort |
Ex Vitro Simultaneous Acclimatization and Rooting of In Vitro Propagated Tamarillo Plants (Solanum betaceum Cav.): Effect of the Substrate and Mineral Nutrition |
author |
Pirata, Madalena Salgado |
author_facet |
Pirata, Madalena Salgado Correia, Sandra Canhoto, Jorge |
author_role |
author |
author2 |
Correia, Sandra Canhoto, Jorge |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Pirata, Madalena Salgado Correia, Sandra Canhoto, Jorge |
dc.subject.por.fl_str_mv |
chlorophyll content controlled-release fertilizer dry biomass in vitro micropropagation quick-release fertilizer |
topic |
chlorophyll content controlled-release fertilizer dry biomass in vitro micropropagation quick-release fertilizer |
description |
Plants propagated by seed do not ensure genetic uniformity and are sometimes infected with diseases. In Vitro micropropagation techniques are an alternative method to traditional cloning approaches for producing true-to-type and pathogen-free plants. However, due to the particularities of the in vitro environment, these plants face many challenges, often critical to their survival, to adapt to ex vitro conditions. In this context, four substrates and two types of mineral nutrition (quick-release (QRF) and controlled-release (CRF) fertilizers), as well as their absence were evaluated in the process of acclimatization of Solanum betaceum plants. Stomatal conductance (gs), chlorophyll content index (CCI), and dry biomass of roots, shoots, and entire plants were the parameters analyzed during the acclimatization. The best crop performance (gs, CCI, and dry biomass) were observed in substrates consisting of vermiculite plus the application of mineral nutrients through a CRF, proving that mineral nutrition has the greatest positive impact on the acclimatization process. In these conditions, plants were obtained with a total dry biomass being significantly higher (515.0 mg (QRF) and 635.9 mg (CRF) when compared to the total dry biomass of untreated plants (119.9 mg). The best conditions for this first experiment were replicated in a second test in order to assess the best fertilizer amount suited for plantlet growth. In this case, the best results were obtained with 0.4 g of CRF, in which plants showed a dry biomass of roots (542.7 mg) and a total dry biomass (594.5 mg), which was significantly higher than in the control (183.2 mg and 165.9 mg, respectively) or with other concentrations of CRF (0.8 and 1.6 g). A similar trend was found concerning the CCI (5.3) and gs (72.5 mmol m2 s1) in which 0.4 g CRF gave also the best results when compared with the control (without CRF) or with 0.8 g (4.7 and 56.2 mmol m2 s1) and 1.6 g (4.7 and 52.2 mmol m2 s1) treatments. In general, it was found that tamarillo plantlets acclimatized to 0.4 g of CRF had a faster initial growth and better performance (CCI and gs), with plants ready to go to the greenhouse/field more quickly, thus reducing the time to obtain suitable plants for the market and shortening the production cycle. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/102833 http://hdl.handle.net/10316/102833 https://doi.org/10.3390/agronomy12051082 |
url |
http://hdl.handle.net/10316/102833 https://doi.org/10.3390/agronomy12051082 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134091390484480 |