Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies

Detalhes bibliográficos
Autor(a) principal: Fernandes, Luana
Data de Publicação: 2019
Outros Autores: Pereira, José Alberto, Saraiva, Jorge, Casal, Susana, Ramalhosa, Elsa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.29352/mill0208.08.00205
Resumo: Introduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used.
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spelling Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansiesInfluencia de los solventes de extracción en el contenido de compuestos bioactivos y actividad antioxidante de las flores dos pensamientosInfluência dos solventes de extração no contéudo em compostos bioactivos e actividade antioxidante de flores de amores-perfeitosAgriculture, Food and Veterinary SciencesIntroduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used.Introducción: Los pensamientos (Viola x wittrockiana) son una rica fuente de antioxidantes naturales con efectos beneficiosos sobre la salud humana. Objetivos: El objetivo del presente estudio fue evaluar la influencia de los solventes (agua, metanol y agua:acetona (6:4, v/v) en la extracción de compuestos bioactivos y actividad antioxidante de extractos de pensamientos. Métodos: Los compuestos bioactivos analizados fueron flavonoides, taninos hidrolizados y antocianinas monoméricas. También se analizaron los fenoles totales por el ensayo de capacidad reductora total. La actividad antioxidante fue evaluada por los métodos: secuestro del radical libre 2,2-difenil-1-picrilhidrazilo (DPPH) y poder reductor. El análisis de componentes principales (PCA) fue realizado para diferenciar los extractos de los pensamientos. Resultados: Los solventes que produjeron extractos con los mayores contenidos de taninos hidrolizados y capacidad reductora total fueron el metanol y agua:acetona (6:4, v/v). Los extractos con mayores contenidos de antocianinas monoméricas se obtuvieron utilizando metanol (5.93 mg Cy 3-glu/g peso seco). Para los flavonoides el agua: acetona (6:4, v / v) fue el solvente que hay dado los mejores valores de extracción (115 mg QE / g peso seco). El agua fue el solvente menos eficaz, obteniéndo se extractos con menor actividad antioxidante. Además, los extractos de metanol y agua:acetona se separaron claramente de los acuosos mediante el análisis por PCA. Conclusións: Los resultados mostraron que la extracción de compuestos bioactivos y la actividad antioxidante de los pensamientos son influenciadas por el solvente utilizado.Introdução: Amores-perfeitos (Viola×wittrockiana) são uma fonte rica de antioxidantes naturais com efeitos benéficos para a saúde humana. Objetivos: O objetivo do presente estudo foi investigar a influência dos solventes (água, metanol e água:acetona (6:4, v/v)) na extracção de compostos bioactivos e actividade antioxidante de extractos de amores-perfeitos. Métodos: Os compostos bioactivos analisados foram os seguintes: flavonóides, taninos hidrolisáveis e antocianinas monoméricas, bem como, os fenóis totais através da avaliação da capacidade redutora total. A actividade antioxidante foi avaliada pelos métodos de sequestro do radical livre 2,2-difenil-1-picrilhidrazilo (DPPH) e poder redutor. Uma análise de componentes principais (PCA) foi realizada para diferenciar os extractos dos amores-perfeitos. Resultados: Os solventes que produziram extractos com os maiores teores de taninos hidrolisáveis e capacidade redutora total foram os de metanol e água: acetona (6:4, v/v). Para extrair os maiores teores de antocianinas monoméricas deve-se usar metanol (5.93 mg Cy 3-glu/ g de massa seca), enquanto que para extrair flavonóides, a água:acetona (6:4, v/v) é preferível (115 mg QE/g de massa seca). A água mostrou ser o solvente menos eficaz, obtendo-se extractos com a menor actividade antioxidante. Para além disso, os extractos de metanol e água:acetona foram claramente separados dos aquosos através da análise por PCA. Conclusões: Os resultados mostraram que a extracção de compostos bioactivos e a actividade antioxidante de flores de amores-perfeitos são influenciadas pelo solvente usado.Polytechnic Institute of Viseu (IPV)2019-01-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.29352/mill0208.08.00205eng1647-662X0873-3015Fernandes, LuanaPereira, José AlbertoSaraiva, JorgeCasal, SusanaRamalhosa, Elsainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-06-15T15:01:46Zoai:ojs.revistas.rcaap.pt:article/14752Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:29:25.900860Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
Influencia de los solventes de extracción en el contenido de compuestos bioactivos y actividad antioxidante de las flores dos pensamientos
Influência dos solventes de extração no contéudo em compostos bioactivos e actividade antioxidante de flores de amores-perfeitos
title Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
spellingShingle Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
Fernandes, Luana
Agriculture, Food and Veterinary Sciences
title_short Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
title_full Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
title_fullStr Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
title_full_unstemmed Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
title_sort Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies
author Fernandes, Luana
author_facet Fernandes, Luana
Pereira, José Alberto
Saraiva, Jorge
Casal, Susana
Ramalhosa, Elsa
author_role author
author2 Pereira, José Alberto
Saraiva, Jorge
Casal, Susana
Ramalhosa, Elsa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Luana
Pereira, José Alberto
Saraiva, Jorge
Casal, Susana
Ramalhosa, Elsa
dc.subject.por.fl_str_mv Agriculture, Food and Veterinary Sciences
topic Agriculture, Food and Veterinary Sciences
description Introduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-31
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://doi.org/10.29352/mill0208.08.00205
url https://doi.org/10.29352/mill0208.08.00205
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1647-662X
0873-3015
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dc.publisher.none.fl_str_mv Polytechnic Institute of Viseu (IPV)
publisher.none.fl_str_mv Polytechnic Institute of Viseu (IPV)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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