Experimental studies on the mechanisms of tiaprofenic acid photosensitization

Detalhes bibliográficos
Autor(a) principal: Figueiredo, A
Data de Publicação: 1993
Outros Autores: Fontes-Ribeiro, CA, Gonçalo, Margarida, Poiares-Baptista, A, Teixeira, F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.4/682
Resumo: Red blood cell lysis and histidine degradation, photosensitized by tiaprofenic acid (TIA), were investigated. Photohaemolysis was markedly enhanced in oxygenated solutions, but was also intense in the presence of nitrogen. Photohaemolysis was inhibited by butylated hydroxyanisole and reduced glutathione, but was unaffected by sodium azide, superoxide dismutase and mannitol. The TIA-induced photo-oxidation of histidine was greatly enhanced in the presence of oxygen and almost completely inhibited in solutions bubbled with nitrogen. Sodium azide, butylated hydroxyanisole and reduced glutathione inhibited the photodegradation of histidine. Phototoxicity to histidine was unaffected by mannitol and superoxide dismutase. The overall results suggest that molecular mechanisms involving free radicals and singlet oxygen are responsible for TIA-photosensitized reactions. These two in vitro models (photohaemolysis and histidine degradation) represent different mechanisms of phototoxicity, but complement one another in the investigation of potential phototoxic substances.
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spelling Experimental studies on the mechanisms of tiaprofenic acid photosensitizationFotossensibilizadoresRed blood cell lysis and histidine degradation, photosensitized by tiaprofenic acid (TIA), were investigated. Photohaemolysis was markedly enhanced in oxygenated solutions, but was also intense in the presence of nitrogen. Photohaemolysis was inhibited by butylated hydroxyanisole and reduced glutathione, but was unaffected by sodium azide, superoxide dismutase and mannitol. The TIA-induced photo-oxidation of histidine was greatly enhanced in the presence of oxygen and almost completely inhibited in solutions bubbled with nitrogen. Sodium azide, butylated hydroxyanisole and reduced glutathione inhibited the photodegradation of histidine. Phototoxicity to histidine was unaffected by mannitol and superoxide dismutase. The overall results suggest that molecular mechanisms involving free radicals and singlet oxygen are responsible for TIA-photosensitized reactions. These two in vitro models (photohaemolysis and histidine degradation) represent different mechanisms of phototoxicity, but complement one another in the investigation of potential phototoxic substances.ElsevierRIHUCFigueiredo, AFontes-Ribeiro, CAGonçalo, MargaridaPoiares-Baptista, ATeixeira, F2009-11-19T13:13:56Z19931993-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.4/682engJ Photochem Photobiol B. 1993 May;18(2-3):161-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-11T14:21:51Zoai:rihuc.huc.min-saude.pt:10400.4/682Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:03:14.549598Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Experimental studies on the mechanisms of tiaprofenic acid photosensitization
title Experimental studies on the mechanisms of tiaprofenic acid photosensitization
spellingShingle Experimental studies on the mechanisms of tiaprofenic acid photosensitization
Figueiredo, A
Fotossensibilizadores
title_short Experimental studies on the mechanisms of tiaprofenic acid photosensitization
title_full Experimental studies on the mechanisms of tiaprofenic acid photosensitization
title_fullStr Experimental studies on the mechanisms of tiaprofenic acid photosensitization
title_full_unstemmed Experimental studies on the mechanisms of tiaprofenic acid photosensitization
title_sort Experimental studies on the mechanisms of tiaprofenic acid photosensitization
author Figueiredo, A
author_facet Figueiredo, A
Fontes-Ribeiro, CA
Gonçalo, Margarida
Poiares-Baptista, A
Teixeira, F
author_role author
author2 Fontes-Ribeiro, CA
Gonçalo, Margarida
Poiares-Baptista, A
Teixeira, F
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RIHUC
dc.contributor.author.fl_str_mv Figueiredo, A
Fontes-Ribeiro, CA
Gonçalo, Margarida
Poiares-Baptista, A
Teixeira, F
dc.subject.por.fl_str_mv Fotossensibilizadores
topic Fotossensibilizadores
description Red blood cell lysis and histidine degradation, photosensitized by tiaprofenic acid (TIA), were investigated. Photohaemolysis was markedly enhanced in oxygenated solutions, but was also intense in the presence of nitrogen. Photohaemolysis was inhibited by butylated hydroxyanisole and reduced glutathione, but was unaffected by sodium azide, superoxide dismutase and mannitol. The TIA-induced photo-oxidation of histidine was greatly enhanced in the presence of oxygen and almost completely inhibited in solutions bubbled with nitrogen. Sodium azide, butylated hydroxyanisole and reduced glutathione inhibited the photodegradation of histidine. Phototoxicity to histidine was unaffected by mannitol and superoxide dismutase. The overall results suggest that molecular mechanisms involving free radicals and singlet oxygen are responsible for TIA-photosensitized reactions. These two in vitro models (photohaemolysis and histidine degradation) represent different mechanisms of phototoxicity, but complement one another in the investigation of potential phototoxic substances.
publishDate 1993
dc.date.none.fl_str_mv 1993
1993-01-01T00:00:00Z
2009-11-19T13:13:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.4/682
url http://hdl.handle.net/10400.4/682
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Photochem Photobiol B. 1993 May;18(2-3):161-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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