First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal
| Autor(a) principal: | |
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| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.1186/s13071-015-0760-2 |
Resumo: | PMID:25886610 WOS:000351904500001 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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First molecular identification of mosquito vectors of Dirofilaria immitis in continental PortugalAe. caspiusAe. detritus s.lAn. maculippenis s.lCx. pipiensCx. theileriDDUDirofilariaMosquitoesPortugalVectorsParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingPMID:25886610 WOS:000351904500001Background: Canine dirofilariasis due to Dirofilaria immitis is known to be endemic in continental Portugal. However, information about the transmitting mosquito species is still scarce, with only Culex theileri identified to date, albeit with L1-2, through dissection. This study was carried out to investigate the potential vectors of Dirofilaria spp. in continental Portugal. Methods: Mosquitoes were collected in three distinct seasons (Summer, Autumn and Spring), 2011-2013, in three districts. CDC traps and indoor resting collections were carried out in the vicinity of kennels. Mosquitoes were kept under controlled conditions for 7 days to allow the development of larval stages of Dirofilaria spp. DNA extraction was performed separately for both head+thorax and abdomen in order to differentiate infective and infected specimens, respectively, in pools, grouped according to the species and collection site (1-40 specimen parts/pool), and examined by PCR using pan-filarial specific primers. Mosquito densities were compared using non-parametric tests. Dirofilaria development units (DDU) were estimated. Results: In total, 9156 female mosquitoes, from 11 different species, were captured. Mosquito densities varied among the 3 districts, according to capture method, and were generally higher in the second year of collections. From 5866 specimens screened by PCR, 23 head+thorax and 41 abdomens pools, corresponding to 54 mosquitoes were found positive for D. immitis DNA. These belonged to 5 species: Culex (Cux) theileri (estimated rate of infection (ERI)=0.71%), Cx. (Cux) pipiens f. pipiens and f. molestus (ERI=0.5%), Anopheles (Ano) maculipennis s.l. (ERI=3.12%), including An. (Ano) atroparvus, Aedes (Och) caspius (ERI=3.73%) and Ae. (Och) detritus s.l. (ERI=4.39%). All but Cx. pipiens, had at least one infective specimen. No D. repens infected specimens were found. Infection rates were: 3.21% in Coimbra, 1.22% in Setúbal and 0.54% in Santarém. DDU were at least 117/year in the study period. Conclusions: Culex theileri, Cx. pipiens, An. maculipennis s.l. An. atroparvus, Ae.caspius and Ae. detritus s.l. were identified as potential vectors of D. immitis in three districts of Portugal, from Spring to Autumn, in 5 of the 6 collection dates in 2011-2013. Implications for transmission, in the context of climate changes, and need for prophylactic measures, are discussed.Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)RUNFerreira, Cátia Alexandra CostaDe Pinho Mixão, VerónicaNovo, Maria Teresa Lourenc¸o MarquesCalado, Maria Manuela PalmeiroGonc¸alves, Luzia Augusta PiresBelo, Silvana Maria DuarteDe Almeida, António Paulo Gouveia2018-05-14T22:01:43Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1186/s13071-015-0760-2engPURE: 1663114http://www.scopus.com/inward/record.url?scp=84925455432&partnerID=8YFLogxKhttps://doi.org/10.1186/s13071-015-0760-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:28Zoai:run.unl.pt:10362/36930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:42.367419Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| title |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| spellingShingle |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal Ferreira, Cátia Alexandra Costa Ae. caspius Ae. detritus s.l An. maculippenis s.l Cx. pipiens Cx. theileri DDU Dirofilaria Mosquitoes Portugal Vectors Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
| title_short |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| title_full |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| title_fullStr |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| title_full_unstemmed |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| title_sort |
First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal |
| author |
Ferreira, Cátia Alexandra Costa |
| author_facet |
Ferreira, Cátia Alexandra Costa De Pinho Mixão, Verónica Novo, Maria Teresa Lourenc¸o Marques Calado, Maria Manuela Palmeiro Gonc¸alves, Luzia Augusta Pires Belo, Silvana Maria Duarte De Almeida, António Paulo Gouveia |
| author_role |
author |
| author2 |
De Pinho Mixão, Verónica Novo, Maria Teresa Lourenc¸o Marques Calado, Maria Manuela Palmeiro Gonc¸alves, Luzia Augusta Pires Belo, Silvana Maria Duarte De Almeida, António Paulo Gouveia |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Vector borne diseases and pathogens (VBD) Global Health and Tropical Medicine (GHTM) RUN |
| dc.contributor.author.fl_str_mv |
Ferreira, Cátia Alexandra Costa De Pinho Mixão, Verónica Novo, Maria Teresa Lourenc¸o Marques Calado, Maria Manuela Palmeiro Gonc¸alves, Luzia Augusta Pires Belo, Silvana Maria Duarte De Almeida, António Paulo Gouveia |
| dc.subject.por.fl_str_mv |
Ae. caspius Ae. detritus s.l An. maculippenis s.l Cx. pipiens Cx. theileri DDU Dirofilaria Mosquitoes Portugal Vectors Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
| topic |
Ae. caspius Ae. detritus s.l An. maculippenis s.l Cx. pipiens Cx. theileri DDU Dirofilaria Mosquitoes Portugal Vectors Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
| description |
PMID:25886610 WOS:000351904500001 |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2018-05-14T22:01:43Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1186/s13071-015-0760-2 |
| url |
https://doi.org/10.1186/s13071-015-0760-2 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 1663114 http://www.scopus.com/inward/record.url?scp=84925455432&partnerID=8YFLogxK https://doi.org/10.1186/s13071-015-0760-2 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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