Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/61272 |
Resumo: | Resveratrol (RSV) is a natural antioxidant commonly found in grapes, berries, and nuts that has shown promising results in the treatment of a variety of degenerative and age-related diseases. Despite the proven beneficial results on reduction of reactive oxidant species (ROS) and on inflammatory process, RSV shows various limitations including low long-term stability, aqueous solubility, and bioavailability, restricting its applications in the medical-pharmaceutical area. To overcome these limitations, it has been applied in pharmaceutical formulations as nanostructured lipid carriers (NLC). Thus, the present study focuses on the optimization of the production process of NLC. NLC was produced by high shear homogenization (HSH) and ultrasound method (US) using Compritol® ATO C888 as solid lipid and Miglyol 812® as liquid lipid. In order to obtain an optimized formulation, we used a 22 full factorial design with triplicate of central point investigating the effects of the production process parameters; shear intensity and homogenization time, on the mean particle size (PS) and polydispersity index (PDI). Instability index, encapsulation efficiency, and production yield were also evaluated. As the PS and PDI values obtained with 6 min of shear at 19,000 rpm and 10 min of shear and 24,000 rpm were similar, the instability index (<0.1) was also used to select the optimal parameters. Based on the results of the experimental design and instability index, it was concluded that the shear rate of 19,000 rpm and the shear time of 6 min are the optimal parameters for RSV-loaded NLC production. Factorial design contributed therefore to optimize the variables of the NLC production process from a small number of experiments. |
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Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial designresveratrolnanostructured lipid carriers (NLC)factorial designhigh shear homogenizationultrasound methodScience & TechnologyResveratrol (RSV) is a natural antioxidant commonly found in grapes, berries, and nuts that has shown promising results in the treatment of a variety of degenerative and age-related diseases. Despite the proven beneficial results on reduction of reactive oxidant species (ROS) and on inflammatory process, RSV shows various limitations including low long-term stability, aqueous solubility, and bioavailability, restricting its applications in the medical-pharmaceutical area. To overcome these limitations, it has been applied in pharmaceutical formulations as nanostructured lipid carriers (NLC). Thus, the present study focuses on the optimization of the production process of NLC. NLC was produced by high shear homogenization (HSH) and ultrasound method (US) using Compritol® ATO C888 as solid lipid and Miglyol 812® as liquid lipid. In order to obtain an optimized formulation, we used a 22 full factorial design with triplicate of central point investigating the effects of the production process parameters; shear intensity and homogenization time, on the mean particle size (PS) and polydispersity index (PDI). Instability index, encapsulation efficiency, and production yield were also evaluated. As the PS and PDI values obtained with 6 min of shear at 19,000 rpm and 10 min of shear and 24,000 rpm were similar, the instability index (<0.1) was also used to select the optimal parameters. Based on the results of the experimental design and instability index, it was concluded that the shear rate of 19,000 rpm and the shear time of 6 min are the optimal parameters for RSV-loaded NLC production. Factorial design contributed therefore to optimize the variables of the NLC production process from a small number of experiments.The work has received financial support from FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), Project’s numbers 2017/034968 and 2014/27200-2 and from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and co-financed by FEDER, under the Partnership Agreement PT2020 for the project M-ERA-NET/0004/2015-PAIRED.info:eu-repo/semantics/publishedVersionMDPI AGUniversidade do MinhoShimojo, Andréa A. M.Fernandes, Ana Rita V.Ferreira, Nuno R. E.Sanchez-Lopez, ElenaSantana, Maria H. A.Souto, Eliana B.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61272engShimojo, Andréa A. M.; Fernandes, Ana Rita V.; Ferreira, Nuno R. E.; Sanchez-Lopez, Elena; Santana, Maria H. A.; Souto, Eliana, Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design. Antioxidants, 8(8), 272, 20192076-392110.3390/antiox8080272https://www.mdpi.com/2076-3921/8/8/272/htminfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:18Zoai:repositorium.sdum.uminho.pt:1822/61272Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:31.684982Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
title |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
spellingShingle |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design Shimojo, Andréa A. M. resveratrol nanostructured lipid carriers (NLC) factorial design high shear homogenization ultrasound method Science & Technology |
title_short |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
title_full |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
title_fullStr |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
title_full_unstemmed |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
title_sort |
Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design |
author |
Shimojo, Andréa A. M. |
author_facet |
Shimojo, Andréa A. M. Fernandes, Ana Rita V. Ferreira, Nuno R. E. Sanchez-Lopez, Elena Santana, Maria H. A. Souto, Eliana B. |
author_role |
author |
author2 |
Fernandes, Ana Rita V. Ferreira, Nuno R. E. Sanchez-Lopez, Elena Santana, Maria H. A. Souto, Eliana B. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Shimojo, Andréa A. M. Fernandes, Ana Rita V. Ferreira, Nuno R. E. Sanchez-Lopez, Elena Santana, Maria H. A. Souto, Eliana B. |
dc.subject.por.fl_str_mv |
resveratrol nanostructured lipid carriers (NLC) factorial design high shear homogenization ultrasound method Science & Technology |
topic |
resveratrol nanostructured lipid carriers (NLC) factorial design high shear homogenization ultrasound method Science & Technology |
description |
Resveratrol (RSV) is a natural antioxidant commonly found in grapes, berries, and nuts that has shown promising results in the treatment of a variety of degenerative and age-related diseases. Despite the proven beneficial results on reduction of reactive oxidant species (ROS) and on inflammatory process, RSV shows various limitations including low long-term stability, aqueous solubility, and bioavailability, restricting its applications in the medical-pharmaceutical area. To overcome these limitations, it has been applied in pharmaceutical formulations as nanostructured lipid carriers (NLC). Thus, the present study focuses on the optimization of the production process of NLC. NLC was produced by high shear homogenization (HSH) and ultrasound method (US) using Compritol® ATO C888 as solid lipid and Miglyol 812® as liquid lipid. In order to obtain an optimized formulation, we used a 22 full factorial design with triplicate of central point investigating the effects of the production process parameters; shear intensity and homogenization time, on the mean particle size (PS) and polydispersity index (PDI). Instability index, encapsulation efficiency, and production yield were also evaluated. As the PS and PDI values obtained with 6 min of shear at 19,000 rpm and 10 min of shear and 24,000 rpm were similar, the instability index (<0.1) was also used to select the optimal parameters. Based on the results of the experimental design and instability index, it was concluded that the shear rate of 19,000 rpm and the shear time of 6 min are the optimal parameters for RSV-loaded NLC production. Factorial design contributed therefore to optimize the variables of the NLC production process from a small number of experiments. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/61272 |
url |
http://hdl.handle.net/1822/61272 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Shimojo, Andréa A. M.; Fernandes, Ana Rita V.; Ferreira, Nuno R. E.; Sanchez-Lopez, Elena; Santana, Maria H. A.; Souto, Eliana, Evaluation of the influence of process parameters on the properties of resveratrol-loaded NLC using 22 full factorial design. Antioxidants, 8(8), 272, 2019 2076-3921 10.3390/antiox8080272 https://www.mdpi.com/2076-3921/8/8/272/htm |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI AG |
publisher.none.fl_str_mv |
MDPI AG |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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