Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/8052 |
Resumo: | In the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPaseCisplatinCa2+-ATPaseIn the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs.Aureliano, M.Bebianno, Maria JoãoSapientiaMorais, Matilde2016-04-21T15:50:44Z20152015-01-01T00:00:00Zbachelor thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10400.1/8052enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:55:11Zoai:sapientia.ualg.pt:10400.1/8052Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:55:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
title |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
spellingShingle |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase Morais, Matilde Cisplatin Ca2+-ATPase |
title_short |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
title_full |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
title_fullStr |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
title_full_unstemmed |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
title_sort |
Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase |
author |
Morais, Matilde |
author_facet |
Morais, Matilde |
author_role |
author |
dc.contributor.none.fl_str_mv |
Aureliano, M. Bebianno, Maria João Sapientia |
dc.contributor.author.fl_str_mv |
Morais, Matilde |
dc.subject.por.fl_str_mv |
Cisplatin Ca2+-ATPase |
topic |
Cisplatin Ca2+-ATPase |
description |
In the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2016-04-21T15:50:44Z |
dc.type.driver.fl_str_mv |
bachelor thesis |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/8052 |
url |
http://hdl.handle.net/10400.1/8052 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817549861683200000 |