Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase

Detalhes bibliográficos
Autor(a) principal: Morais, Matilde
Data de Publicação: 2015
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/8052
Resumo: In the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs.
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spelling Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPaseCisplatinCa2+-ATPaseIn the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs.Aureliano, M.Bebianno, Maria JoãoSapientiaMorais, Matilde2016-04-21T15:50:44Z20152015-01-01T00:00:00Zbachelor thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10400.1/8052enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:55:11Zoai:sapientia.ualg.pt:10400.1/8052Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:55:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
title Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
spellingShingle Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
Morais, Matilde
Cisplatin
Ca2+-ATPase
title_short Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
title_full Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
title_fullStr Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
title_full_unstemmed Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
title_sort Effect of cisplatin in Mytilus galloprovincialis and of cyclophosphamide, tamoxifen and cisplatin on Ca2+ATPase
author Morais, Matilde
author_facet Morais, Matilde
author_role author
dc.contributor.none.fl_str_mv Aureliano, M.
Bebianno, Maria João
Sapientia
dc.contributor.author.fl_str_mv Morais, Matilde
dc.subject.por.fl_str_mv Cisplatin
Ca2+-ATPase
topic Cisplatin
Ca2+-ATPase
description In the last decade anti-cancer drugs, such as cisplatin, tamoxifen and cyclophosphamide, have seen their use greatly increase. Cisplatin is one of the most common anti-cancer drugs used in the EU which ubiquitous occurrence in surface waters, like rivers and estuaries, is related to the poor removal capacities of waste-water treatment plants (WWTPs). These drugs are genotoxic, cytotoxic, mutagenic and teratogenic. Therefore, this study includes a multibiomarker response analysis on mussel Mytilus galloprovincialis during two weeks of exposure to 100ng/l of cisplatin assessing antioxidant enzyme activity - catalase (CAT), glutathion-S-transferase (GST); lipid peroxidation (LPO) coupled with an enzyme assay to determine the inhibition effect of cisplatin, tamoxifen and cyclophosphamide (IC50) in the Ca2+ -ATPase. Results show that cisplatin does not seem to have an effect in the activity of CAT, GST and LPO and that its IC50 is 13.7 mM. The effect of the other two anti-cancer drugs were tamoxifen 0.271 mM and cyclophosphamide 1.134 μM. which makes Cisplatin much less dangerous than the other two anti-cancer drugs.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-04-21T15:50:44Z
dc.type.driver.fl_str_mv bachelor thesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/8052
url http://hdl.handle.net/10400.1/8052
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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