Early detection of grapevine graft incompatibility

Detalhes bibliográficos
Autor(a) principal: Tedesco, Sara
Data de Publicação: 2023
Outros Autores: Irisarri, Patricia, Teixeira Santos, Margarida, Fevereiro, Pedro, Pina, Ana, Kragler, Friedrich
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158667
Resumo: Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Publisher Copyright: © 2023
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spelling Early detection of grapevine graft incompatibilityInsights into translocated and virus-induced incompatibilityGraft incompatibilityGrapevineGRSPaVHistologyIn vitro cultureSyrahHorticultureFunding Information: This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Publisher Copyright: © 2023In vineyards to control phylloxera (Daktulosphaira vitifolia Ficth) attacks in Vitis vinifera L., heterografted vines are planted using American vines hybrids as rootstocks. However, graft incompatibilities can affect grape yield and plant longevity. Thus, to identify early graft incompatibility factors, we established in vitro micrografting protocols coupled with histology and histochemistry analysis in grapevine graft combinations of known compatibility behavior. The histochemical characterization of the graft union revealed irregular cell arrangement, slower vascular differentiation, persistence of the necrotic layer, accumulation of starch, and lower differentiation of phloem cells in hetero- compared to homografts, indicating the presence of translocated incompatibility symptoms. We highlight the utility of evaluating the graft interface cellular arrangement and starch content via calcofluor and I2KI staining, respectively, as allowed to identify the graft combinations with lower graft success. Wounded and grafted Syrah plantlets pointed out an impaired sucrose distribution in these plants and levels of Grapevine Rupestris Stem Pitting associated Virus (GRSPaV) infections correlated with graft (un)-success in two Syrah clones micrografted onto 110-Ritcher rootstock. Furthermore, silencing GRSPaV before grafting increased graft success rates. We propose that grapevine graft incompatibility is mainly a virus-induced phenomenon that can arise even in certified plants.Bioresources 4 Sustainability (GREEN-IT)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNTedesco, SaraIrisarri, PatriciaTeixeira Santos, MargaridaFevereiro, PedroPina, AnaKragler, Friedrich2023-10-03T22:20:11Z2023-08-012023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158667eng0304-4238PURE: 72985298https://doi.org/10.1016/j.scienta.2023.112087info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:13Zoai:run.unl.pt:10362/158667Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:15.990369Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Early detection of grapevine graft incompatibility
Insights into translocated and virus-induced incompatibility
title Early detection of grapevine graft incompatibility
spellingShingle Early detection of grapevine graft incompatibility
Tedesco, Sara
Graft incompatibility
Grapevine
GRSPaV
Histology
In vitro culture
Syrah
Horticulture
title_short Early detection of grapevine graft incompatibility
title_full Early detection of grapevine graft incompatibility
title_fullStr Early detection of grapevine graft incompatibility
title_full_unstemmed Early detection of grapevine graft incompatibility
title_sort Early detection of grapevine graft incompatibility
author Tedesco, Sara
author_facet Tedesco, Sara
Irisarri, Patricia
Teixeira Santos, Margarida
Fevereiro, Pedro
Pina, Ana
Kragler, Friedrich
author_role author
author2 Irisarri, Patricia
Teixeira Santos, Margarida
Fevereiro, Pedro
Pina, Ana
Kragler, Friedrich
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Bioresources 4 Sustainability (GREEN-IT)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Tedesco, Sara
Irisarri, Patricia
Teixeira Santos, Margarida
Fevereiro, Pedro
Pina, Ana
Kragler, Friedrich
dc.subject.por.fl_str_mv Graft incompatibility
Grapevine
GRSPaV
Histology
In vitro culture
Syrah
Horticulture
topic Graft incompatibility
Grapevine
GRSPaV
Histology
In vitro culture
Syrah
Horticulture
description Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Publisher Copyright: © 2023
publishDate 2023
dc.date.none.fl_str_mv 2023-10-03T22:20:11Z
2023-08-01
2023-08-01T00:00:00Z
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dc.language.iso.fl_str_mv eng
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PURE: 72985298
https://doi.org/10.1016/j.scienta.2023.112087
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