Early detection of grapevine graft incompatibility
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/158667 |
Resumo: | Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Publisher Copyright: © 2023 |
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Early detection of grapevine graft incompatibilityInsights into translocated and virus-induced incompatibilityGraft incompatibilityGrapevineGRSPaVHistologyIn vitro cultureSyrahHorticultureFunding Information: This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Publisher Copyright: © 2023In vineyards to control phylloxera (Daktulosphaira vitifolia Ficth) attacks in Vitis vinifera L., heterografted vines are planted using American vines hybrids as rootstocks. However, graft incompatibilities can affect grape yield and plant longevity. Thus, to identify early graft incompatibility factors, we established in vitro micrografting protocols coupled with histology and histochemistry analysis in grapevine graft combinations of known compatibility behavior. The histochemical characterization of the graft union revealed irregular cell arrangement, slower vascular differentiation, persistence of the necrotic layer, accumulation of starch, and lower differentiation of phloem cells in hetero- compared to homografts, indicating the presence of translocated incompatibility symptoms. We highlight the utility of evaluating the graft interface cellular arrangement and starch content via calcofluor and I2KI staining, respectively, as allowed to identify the graft combinations with lower graft success. Wounded and grafted Syrah plantlets pointed out an impaired sucrose distribution in these plants and levels of Grapevine Rupestris Stem Pitting associated Virus (GRSPaV) infections correlated with graft (un)-success in two Syrah clones micrografted onto 110-Ritcher rootstock. Furthermore, silencing GRSPaV before grafting increased graft success rates. We propose that grapevine graft incompatibility is mainly a virus-induced phenomenon that can arise even in certified plants.Bioresources 4 Sustainability (GREEN-IT)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNTedesco, SaraIrisarri, PatriciaTeixeira Santos, MargaridaFevereiro, PedroPina, AnaKragler, Friedrich2023-10-03T22:20:11Z2023-08-012023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158667eng0304-4238PURE: 72985298https://doi.org/10.1016/j.scienta.2023.112087info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:13Zoai:run.unl.pt:10362/158667Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:15.990369Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Early detection of grapevine graft incompatibility Insights into translocated and virus-induced incompatibility |
title |
Early detection of grapevine graft incompatibility |
spellingShingle |
Early detection of grapevine graft incompatibility Tedesco, Sara Graft incompatibility Grapevine GRSPaV Histology In vitro culture Syrah Horticulture |
title_short |
Early detection of grapevine graft incompatibility |
title_full |
Early detection of grapevine graft incompatibility |
title_fullStr |
Early detection of grapevine graft incompatibility |
title_full_unstemmed |
Early detection of grapevine graft incompatibility |
title_sort |
Early detection of grapevine graft incompatibility |
author |
Tedesco, Sara |
author_facet |
Tedesco, Sara Irisarri, Patricia Teixeira Santos, Margarida Fevereiro, Pedro Pina, Ana Kragler, Friedrich |
author_role |
author |
author2 |
Irisarri, Patricia Teixeira Santos, Margarida Fevereiro, Pedro Pina, Ana Kragler, Friedrich |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Bioresources 4 Sustainability (GREEN-IT) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Tedesco, Sara Irisarri, Patricia Teixeira Santos, Margarida Fevereiro, Pedro Pina, Ana Kragler, Friedrich |
dc.subject.por.fl_str_mv |
Graft incompatibility Grapevine GRSPaV Histology In vitro culture Syrah Horticulture |
topic |
Graft incompatibility Grapevine GRSPaV Histology In vitro culture Syrah Horticulture |
description |
Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society, Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021. The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón- European Social Fund, European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Funding Information: This research was supported by internal funds provided to FK from the Max Planck Society , Germany, and by the Fundação para a Ciência e Tecnologia (FCT), Portugal for the Ph.D. grant with the reference PD/BD/128399/2017 and COVID/BD/151639/2021 . The authors acknowledge the research unit GREEN-it “Bioresources for Sustainability” (UID/Multi/04551/2013) and the Gobierno de Aragón - European Social Fund , European Union (Grupo Consolidado A12). The authors thank the National Institute for Agricultural and Veterinary Research (INIAV-Dois Portos) for the provision of the plant material, and Teresa Valdiviesso and Cândida Sofia Trindade (INIAV- Oeiras) for the use of the histology facility. We acknowledge Margarida Basaloco for the reference genes primer design, and Cindy Hauptvogel and Saurabh Gupta in the Kragler laboratory for help in monitoring plants under different sucrose concentrations and analysing RNAseq data for the presence of viral sequences, respectively. Publisher Copyright: © 2023 |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-03T22:20:11Z 2023-08-01 2023-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/158667 |
url |
http://hdl.handle.net/10362/158667 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0304-4238 PURE: 72985298 https://doi.org/10.1016/j.scienta.2023.112087 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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