Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/4950 |
Resumo: | Staphylococcus aureus is a major pathogen in bone associated infections due to its ability to adhere and form biofilms on bone and/or implants. Moreover, recrudescent and chronic infections have been associated with S. aureus capacity to invade and persist within osteoblast cells. With the growing need of novel therapeutic tools, this research aimed to evaluate some important key biological properties of a novel carrier system composed of acrylic bone cement (polymethylmethacrylate - PMMA), loaded with a release modulator (lactose) and an antibiotic (levofloxacin). Levofloxacin-loaded bone cement (BC) exhibited antimicrobial effects against planktonic and biofilm forms of S. aureus (evaluated by a flow chamber system). Moreover, novel BC formulation showed high anti-bacterial intraosteoblast activity. This fact led to the conclusion that levofloxacin released from BC matrices could penetrate the cell membrane of osteoblasts and be active against S. aureus strains in the intracellular environment. Furthermore, levofloxacin-BC formulations showed no significant in vitro cytotoxicity and no allergic potential (measured by the in vivo chorioallantoic membrane assay). Our results indicate that levofloxacin-loaded BC has potential as a local antibiotic delivery system for treating S. aureus associated bone infections. |
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Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilmsCementsLevofloxacinBiofilmsToxicityBiocompatibilityBone-infectionFlow-chamber SystemFluoroquinolone-delivery-systemOsteoblast-infection-modelStaphylococcus aureusStaphylococcus aureus is a major pathogen in bone associated infections due to its ability to adhere and form biofilms on bone and/or implants. Moreover, recrudescent and chronic infections have been associated with S. aureus capacity to invade and persist within osteoblast cells. With the growing need of novel therapeutic tools, this research aimed to evaluate some important key biological properties of a novel carrier system composed of acrylic bone cement (polymethylmethacrylate - PMMA), loaded with a release modulator (lactose) and an antibiotic (levofloxacin). Levofloxacin-loaded bone cement (BC) exhibited antimicrobial effects against planktonic and biofilm forms of S. aureus (evaluated by a flow chamber system). Moreover, novel BC formulation showed high anti-bacterial intraosteoblast activity. This fact led to the conclusion that levofloxacin released from BC matrices could penetrate the cell membrane of osteoblasts and be active against S. aureus strains in the intracellular environment. Furthermore, levofloxacin-BC formulations showed no significant in vitro cytotoxicity and no allergic potential (measured by the in vivo chorioallantoic membrane assay). Our results indicate that levofloxacin-loaded BC has potential as a local antibiotic delivery system for treating S. aureus associated bone infections.Fundação para a Ciência e Tecnologia from Portuguese government (Project PEst-OE/SAU/UI4013/2014). The paper is based upon the work from COST TD1305 (Improved Protection of Medical Devices against infection).ElsevierRepositório Científico do Instituto Nacional de SaúdeFerreira, MagdaRzhepishevska, OlenaGrenho, LilianaMalheiros, DanilaGonçalves, LídiaAlmeida, António J.Jordao, LuisaRibeiro, Isabel A.Ramstedt, MadeleineGomes, PedroBettencourt, Ana2021-01-01T01:30:11Z2017-10-302017-10-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4950engInt J Pharm. 2017 Oct 30;532(1):241-248. doi: 10.1016/j.ijpharm.2017.08.089. Epub 2017 Aug 26.0378-517310.1016/j.ijpharm.2017.08.089info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:32Zoai:repositorio.insa.pt:10400.18/4950Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:35.819315Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
title |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
spellingShingle |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms Ferreira, Magda Cements Levofloxacin Biofilms Toxicity Biocompatibility Bone-infection Flow-chamber System Fluoroquinolone-delivery-system Osteoblast-infection-model Staphylococcus aureus |
title_short |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
title_full |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
title_fullStr |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
title_full_unstemmed |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
title_sort |
Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms |
author |
Ferreira, Magda |
author_facet |
Ferreira, Magda Rzhepishevska, Olena Grenho, Liliana Malheiros, Danila Gonçalves, Lídia Almeida, António J. Jordao, Luisa Ribeiro, Isabel A. Ramstedt, Madeleine Gomes, Pedro Bettencourt, Ana |
author_role |
author |
author2 |
Rzhepishevska, Olena Grenho, Liliana Malheiros, Danila Gonçalves, Lídia Almeida, António J. Jordao, Luisa Ribeiro, Isabel A. Ramstedt, Madeleine Gomes, Pedro Bettencourt, Ana |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Ferreira, Magda Rzhepishevska, Olena Grenho, Liliana Malheiros, Danila Gonçalves, Lídia Almeida, António J. Jordao, Luisa Ribeiro, Isabel A. Ramstedt, Madeleine Gomes, Pedro Bettencourt, Ana |
dc.subject.por.fl_str_mv |
Cements Levofloxacin Biofilms Toxicity Biocompatibility Bone-infection Flow-chamber System Fluoroquinolone-delivery-system Osteoblast-infection-model Staphylococcus aureus |
topic |
Cements Levofloxacin Biofilms Toxicity Biocompatibility Bone-infection Flow-chamber System Fluoroquinolone-delivery-system Osteoblast-infection-model Staphylococcus aureus |
description |
Staphylococcus aureus is a major pathogen in bone associated infections due to its ability to adhere and form biofilms on bone and/or implants. Moreover, recrudescent and chronic infections have been associated with S. aureus capacity to invade and persist within osteoblast cells. With the growing need of novel therapeutic tools, this research aimed to evaluate some important key biological properties of a novel carrier system composed of acrylic bone cement (polymethylmethacrylate - PMMA), loaded with a release modulator (lactose) and an antibiotic (levofloxacin). Levofloxacin-loaded bone cement (BC) exhibited antimicrobial effects against planktonic and biofilm forms of S. aureus (evaluated by a flow chamber system). Moreover, novel BC formulation showed high anti-bacterial intraosteoblast activity. This fact led to the conclusion that levofloxacin released from BC matrices could penetrate the cell membrane of osteoblasts and be active against S. aureus strains in the intracellular environment. Furthermore, levofloxacin-BC formulations showed no significant in vitro cytotoxicity and no allergic potential (measured by the in vivo chorioallantoic membrane assay). Our results indicate that levofloxacin-loaded BC has potential as a local antibiotic delivery system for treating S. aureus associated bone infections. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-30 2017-10-30T00:00:00Z 2021-01-01T01:30:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4950 |
url |
http://hdl.handle.net/10400.18/4950 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Int J Pharm. 2017 Oct 30;532(1):241-248. doi: 10.1016/j.ijpharm.2017.08.089. Epub 2017 Aug 26. 0378-5173 10.1016/j.ijpharm.2017.08.089 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132135615889408 |