Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying

Detalhes bibliográficos
Autor(a) principal: Costa, Clarinda
Data de Publicação: 2023
Outros Autores: Casimiro, Teresa, Corvo, M. Luísa, Aguiar-Ricardo, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/154549
Resumo: Funding Information: C. Costa thanks FCT (Fundação para a Ciência e Tecnologia) and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for Project PD/00184/2012-PDQS . C. Costa and A. Aguiar-Ricardo acknowledge also CA18224 GREENERING (“Green Chemical Engineering Network towards upscaling sustainable processes”). COST Actions are funded within the EU Horizon 2020 Programme . M.L. Corvo is grateful for the financial support of the Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa , Lisbon, Portugal, which is supported in part by UID/DTP/04138/2020 from FCT/MCTES , Portugal. The authors are grateful to Prof. M. Dionísio for the DSC facility. Publisher Copyright: © 2023
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spelling Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-dryingA proof-of-conceptCu, Zn – superoxide dismutaseInflammationPulmonary deliverySolid dosage formsTherapeutic enzymesChemical Engineering(all)Condensed Matter PhysicsPhysical and Theoretical ChemistryFunding Information: C. Costa thanks FCT (Fundação para a Ciência e Tecnologia) and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for Project PD/00184/2012-PDQS . C. Costa and A. Aguiar-Ricardo acknowledge also CA18224 GREENERING (“Green Chemical Engineering Network towards upscaling sustainable processes”). COST Actions are funded within the EU Horizon 2020 Programme . M.L. Corvo is grateful for the financial support of the Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa , Lisbon, Portugal, which is supported in part by UID/DTP/04138/2020 from FCT/MCTES , Portugal. The authors are grateful to Prof. M. Dionísio for the DSC facility. Publisher Copyright: © 2023Enzyme-based inhalable therapeutics for lung inflammation are gaining interest as an alternative to long-term corticosteroids treatments. However, enzymes have poor pharmacokinetics. Encapsulating enzymes in liposomes can increase their half-live and modify their biodistribution. But both liposomes and enzymes are susceptible to destabilization during storage. This drawback can be surpassed, by converting liposomal suspension into solid dosage forms for different administration routes, including inhalation. In this study, Cu, Zn- superoxide dismutase (SOD) was encapsulated in liposomes, then dried using supercritical CO2-assisted spray-drying to make SOD-loaded liposomal dry powder formulations (SOD_Lip-DPFs). Upon resuspension in water, liposomes maintained structural integrity, with 99% SOD encapsulation efficiency and preserved enzymatic activity. Stability studies showed that SOD_Lip-DPFs maintained liposomal and enzyme stability for 50 days at 40% relative humidity. This offers a stable and efficient delivery system for enzyme-based inhalable therapeutics.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNCosta, ClarindaCasimiro, TeresaCorvo, M. LuísaAguiar-Ricardo, Ana2023-06-28T22:17:22Z2023-092023-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/154549eng0896-8446PURE: 64720285https://doi.org/10.1016/j.supflu.2023.105991info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:36:57Zoai:run.unl.pt:10362/154549Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:39.565089Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
A proof-of-concept
title Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
spellingShingle Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
Costa, Clarinda
Cu, Zn – superoxide dismutase
Inflammation
Pulmonary delivery
Solid dosage forms
Therapeutic enzymes
Chemical Engineering(all)
Condensed Matter Physics
Physical and Theoretical Chemistry
title_short Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
title_full Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
title_fullStr Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
title_full_unstemmed Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
title_sort Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
author Costa, Clarinda
author_facet Costa, Clarinda
Casimiro, Teresa
Corvo, M. Luísa
Aguiar-Ricardo, Ana
author_role author
author2 Casimiro, Teresa
Corvo, M. Luísa
Aguiar-Ricardo, Ana
author2_role author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Costa, Clarinda
Casimiro, Teresa
Corvo, M. Luísa
Aguiar-Ricardo, Ana
dc.subject.por.fl_str_mv Cu, Zn – superoxide dismutase
Inflammation
Pulmonary delivery
Solid dosage forms
Therapeutic enzymes
Chemical Engineering(all)
Condensed Matter Physics
Physical and Theoretical Chemistry
topic Cu, Zn – superoxide dismutase
Inflammation
Pulmonary delivery
Solid dosage forms
Therapeutic enzymes
Chemical Engineering(all)
Condensed Matter Physics
Physical and Theoretical Chemistry
description Funding Information: C. Costa thanks FCT (Fundação para a Ciência e Tecnologia) and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for Project PD/00184/2012-PDQS . C. Costa and A. Aguiar-Ricardo acknowledge also CA18224 GREENERING (“Green Chemical Engineering Network towards upscaling sustainable processes”). COST Actions are funded within the EU Horizon 2020 Programme . M.L. Corvo is grateful for the financial support of the Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa , Lisbon, Portugal, which is supported in part by UID/DTP/04138/2020 from FCT/MCTES , Portugal. The authors are grateful to Prof. M. Dionísio for the DSC facility. Publisher Copyright: © 2023
publishDate 2023
dc.date.none.fl_str_mv 2023-06-28T22:17:22Z
2023-09
2023-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/154549
url http://hdl.handle.net/10362/154549
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0896-8446
PURE: 64720285
https://doi.org/10.1016/j.supflu.2023.105991
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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