Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology

Detalhes bibliográficos
Autor(a) principal: Apura, Patrícia de Faria Pais
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/12400
Resumo: Dissertation presented to obtain the Master Degree in Molecular, Genetics and Biomedicine
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spelling Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biologySmall RNAsGene expressionSynthetic biologyTranscriptional controlPost-transcriptional controlDissertation presented to obtain the Master Degree in Molecular, Genetics and BiomedicineTranscriptional and post-transcriptional control of gene expression dictate the levels of proteins in the cell. Therefore the modulation of gene expression can have important consequences for biotechnological and/or pharmaceutical purposes. Among the types of cellular RNAs, small RNAs (sRNAs) have been an emerging class of bacterial gene expression regulators, which mostly act by base-pairing with one or more mRNA target(s) affecting their translation and/or their stability. Here, we focus on the study of SraL sRNA, more specifically in the validation of putative targets for this sRNA obtained in a previous transcriptomic analysis. Until now SraL was only shown to regulate the mRNA levels of Trigger Factor, an important protein chaperone. The information here reported give strong evidence for SraL involvement in the cysteine biosynthetic pathway, which requires further investigation. Nevertheless, our results could not provide a validation of those putative targets previously obtained by transcriptomic analyses. Optimization of protein expression requires not only an increase of the stability of mRNA transcripts but also an optimal behavior of function-encoding DNA segments, which are often context-dependent. Building on the work of others, we have designed a set of combinatorial promoters and 5’UTRs and evaluated their effects/outcomes using Superfolder GFP as reporter. Our data shows a clear variability of protein levels within our set of constructs. The highest levels of protein were associated with the implementation of an insulation sequence flanking the promoter region and the introduction of 5’ stabilizing structures at the mRNA level. Further investigation concerning the alteration of the rate of the mRNA decay by depletion of the function of participating nucleases, might constitute an advantageous approach. The knowledge collected will be extremely important to design robust modules which substantially increase protein production. This field is rapidly growing and much remains to be discovered about these important regulatory processes.Faculdade de Ciências e TecnologiaArraiano, CecíliaViegas, SandraSilva, InêsRUNApura, Patrícia de Faria Pais2014-07-08T10:17:04Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/mswordhttp://hdl.handle.net/10362/12400enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:47:18Zoai:run.unl.pt:10362/12400Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:20:53.134904Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
title Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
spellingShingle Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
Apura, Patrícia de Faria Pais
Small RNAs
Gene expression
Synthetic biology
Transcriptional control
Post-transcriptional control
title_short Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
title_full Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
title_fullStr Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
title_full_unstemmed Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
title_sort Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology
author Apura, Patrícia de Faria Pais
author_facet Apura, Patrícia de Faria Pais
author_role author
dc.contributor.none.fl_str_mv Arraiano, Cecília
Viegas, Sandra
Silva, Inês
RUN
dc.contributor.author.fl_str_mv Apura, Patrícia de Faria Pais
dc.subject.por.fl_str_mv Small RNAs
Gene expression
Synthetic biology
Transcriptional control
Post-transcriptional control
topic Small RNAs
Gene expression
Synthetic biology
Transcriptional control
Post-transcriptional control
description Dissertation presented to obtain the Master Degree in Molecular, Genetics and Biomedicine
publishDate 2014
dc.date.none.fl_str_mv 2014-07-08T10:17:04Z
2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/12400
url http://hdl.handle.net/10362/12400
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/msword
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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