Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions

Detalhes bibliográficos
Autor(a) principal: De Castro, Aline M.
Data de Publicação: 2020
Outros Autores: Ferreira, Elisabete, Portugal, Carla, Neves, Luisa A., Crespo, João G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/120023
Resumo: UIDB/50006/2020 IF/00505/2014/CP1224/CT0004
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spelling Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditionsCarbonic anhydraseCircular dichroismCO captureFluorescenceGreenhouse gasesCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistryUIDB/50006/2020 IF/00505/2014/CP1224/CT0004The unprecedently high CO2 levels in the atmosphere evoke the urgent need for development of technologies for mitigation of its emissions. Among the alternatives, the biocatalytic route has been claimed as one of the most promising. In the present work, the carbonic anhydrase from bovine erythrocytes (BCA) was employed as a model enzyme for structural studies in an aqueous phase at alkaline pH, which is typical of large-scale absorption processes under operation. Circular dichroism (CD) analysis revealed a high enzymatic stability at pH 10 with a prominent decrease of the melting temperature above this value. The CO2 absorption capacity of the aqueous solutions were assessed by online monitoring of pressure decay in a stainless-steel cell, which indicated a better performance at pH 10 with a kinetic rate increase of up to 43%, as compared to non-biocatalytic conditions. Even low enzyme concentrations (0.2 mg g−1) proved to be sufficient to improve the overall CO2 capture process performance. The enzyme-enhanced approach of CO2 capture presents a high potential and should be further studied.LAQV@REQUIMTEDQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNDe Castro, Aline M.Ferreira, ElisabetePortugal, CarlaNeves, Luisa A.Crespo, João G.2021-06-25T22:17:56Z2020-04-222020-04-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120023eng1661-6596PURE: 28981029https://doi.org/10.3390/ijms21082918info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:02:40Zoai:run.unl.pt:10362/120023Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:16.327675Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
title Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
spellingShingle Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
De Castro, Aline M.
Carbonic anhydrase
Circular dichroism
CO capture
Fluorescence
Greenhouse gases
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
title_short Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
title_full Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
title_fullStr Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
title_full_unstemmed Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
title_sort Biocatalytic CO2 absorption and structural studies of carbonic anhydrase under industrially-relevant conditions
author De Castro, Aline M.
author_facet De Castro, Aline M.
Ferreira, Elisabete
Portugal, Carla
Neves, Luisa A.
Crespo, João G.
author_role author
author2 Ferreira, Elisabete
Portugal, Carla
Neves, Luisa A.
Crespo, João G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv De Castro, Aline M.
Ferreira, Elisabete
Portugal, Carla
Neves, Luisa A.
Crespo, João G.
dc.subject.por.fl_str_mv Carbonic anhydrase
Circular dichroism
CO capture
Fluorescence
Greenhouse gases
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
topic Carbonic anhydrase
Circular dichroism
CO capture
Fluorescence
Greenhouse gases
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
description UIDB/50006/2020 IF/00505/2014/CP1224/CT0004
publishDate 2020
dc.date.none.fl_str_mv 2020-04-22
2020-04-22T00:00:00Z
2021-06-25T22:17:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/120023
url http://hdl.handle.net/10362/120023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 28981029
https://doi.org/10.3390/ijms21082918
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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