The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats

Detalhes bibliográficos
Autor(a) principal: Lima, Cristóvão F.
Data de Publicação: 2005
Outros Autores: Andrade, Paula B., Seabra, Rosa M., Ferreira, Manuel Fernandes, Wilson, Cristina Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/4143
Resumo: In this study we evaluate the biosafety and bioactivity (antioxidant potential) of a traditional water infusion (tea) of common sage (Salvia officinalis L.) in vivo in mice and rats by quantification of plasma transaminase activities and liver GST and GR enzyme activities. The replacement of water by sage tea for 14 days in the diet of rodents did not affect the body weight and food consumption and did not induce liver toxicity. On the other hand, a significant increase of liver GST activity was observed in rats (24%) and mice (10%) of sage drinking groups. The antioxidant potential of sage tea drinking was also studied in vitro in a model using rat hepatocytes in primary culture. The replacement of drinking water with sage tea in the rats used as hepatocyte donors resulted in an improvement of the antioxidant status of rat hepatocytes in primary culture, namely a significant increase in GSH content and GST activity after 4 hours of culture. When these hepatocyte cultures were exposed to 0.75 or 1 mM of tert-butyl hydroperoxide for 1 hour, some protection against lipid peroxidation and GSH depletion was conferred by sage tea drinking. However, the cell death induced by t-BHP as shown by LDH leakage was not different from that observed in cultures from control animals. This study indicates that the compounds present in this sage preparation contain interesting bioactivities which improve the liver antioxidant potential.
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spelling The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and ratsSalvia officinalis L. infusionGlutathione statusAntioxidant effectsRat hepatocytesMiceTert-butyl hydroperoxideScience & TechnologyIn this study we evaluate the biosafety and bioactivity (antioxidant potential) of a traditional water infusion (tea) of common sage (Salvia officinalis L.) in vivo in mice and rats by quantification of plasma transaminase activities and liver GST and GR enzyme activities. The replacement of water by sage tea for 14 days in the diet of rodents did not affect the body weight and food consumption and did not induce liver toxicity. On the other hand, a significant increase of liver GST activity was observed in rats (24%) and mice (10%) of sage drinking groups. The antioxidant potential of sage tea drinking was also studied in vitro in a model using rat hepatocytes in primary culture. The replacement of drinking water with sage tea in the rats used as hepatocyte donors resulted in an improvement of the antioxidant status of rat hepatocytes in primary culture, namely a significant increase in GSH content and GST activity after 4 hours of culture. When these hepatocyte cultures were exposed to 0.75 or 1 mM of tert-butyl hydroperoxide for 1 hour, some protection against lipid peroxidation and GSH depletion was conferred by sage tea drinking. However, the cell death induced by t-BHP as shown by LDH leakage was not different from that observed in cultures from control animals. This study indicates that the compounds present in this sage preparation contain interesting bioactivities which improve the liver antioxidant potential.Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/6942/2001, Programa Operacional “Ciência, Tecnologia, Inovação” (POCTI) - POCTI/AGR/43482/2001.ElsevierUniversidade do MinhoLima, Cristóvão F.Andrade, Paula B.Seabra, Rosa M.Ferreira, Manuel FernandesWilson, Cristina Pereira2005-02-282005-02-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/4143engLima, C. F., Andrade, P. B., Seabra, R. M., Fernandes-Ferreira, M., & Pereira-Wilson, C. (2005). The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. Journal of Ethnopharmacology, 97(2), 383-389. doi: https://doi.org/10.1016/j.jep.2004.11.0290378-874110.1016/j.jep.2004.11.02915707779https://www.sciencedirect.com/science/article/abs/pii/S0378874104005902info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:01Zoai:repositorium.sdum.uminho.pt:1822/4143Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:44.830862Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
title The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
spellingShingle The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
Lima, Cristóvão F.
Salvia officinalis L. infusion
Glutathione status
Antioxidant effects
Rat hepatocytes
Mice
Tert-butyl hydroperoxide
Science & Technology
title_short The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
title_full The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
title_fullStr The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
title_full_unstemmed The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
title_sort The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats
author Lima, Cristóvão F.
author_facet Lima, Cristóvão F.
Andrade, Paula B.
Seabra, Rosa M.
Ferreira, Manuel Fernandes
Wilson, Cristina Pereira
author_role author
author2 Andrade, Paula B.
Seabra, Rosa M.
Ferreira, Manuel Fernandes
Wilson, Cristina Pereira
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lima, Cristóvão F.
Andrade, Paula B.
Seabra, Rosa M.
Ferreira, Manuel Fernandes
Wilson, Cristina Pereira
dc.subject.por.fl_str_mv Salvia officinalis L. infusion
Glutathione status
Antioxidant effects
Rat hepatocytes
Mice
Tert-butyl hydroperoxide
Science & Technology
topic Salvia officinalis L. infusion
Glutathione status
Antioxidant effects
Rat hepatocytes
Mice
Tert-butyl hydroperoxide
Science & Technology
description In this study we evaluate the biosafety and bioactivity (antioxidant potential) of a traditional water infusion (tea) of common sage (Salvia officinalis L.) in vivo in mice and rats by quantification of plasma transaminase activities and liver GST and GR enzyme activities. The replacement of water by sage tea for 14 days in the diet of rodents did not affect the body weight and food consumption and did not induce liver toxicity. On the other hand, a significant increase of liver GST activity was observed in rats (24%) and mice (10%) of sage drinking groups. The antioxidant potential of sage tea drinking was also studied in vitro in a model using rat hepatocytes in primary culture. The replacement of drinking water with sage tea in the rats used as hepatocyte donors resulted in an improvement of the antioxidant status of rat hepatocytes in primary culture, namely a significant increase in GSH content and GST activity after 4 hours of culture. When these hepatocyte cultures were exposed to 0.75 or 1 mM of tert-butyl hydroperoxide for 1 hour, some protection against lipid peroxidation and GSH depletion was conferred by sage tea drinking. However, the cell death induced by t-BHP as shown by LDH leakage was not different from that observed in cultures from control animals. This study indicates that the compounds present in this sage preparation contain interesting bioactivities which improve the liver antioxidant potential.
publishDate 2005
dc.date.none.fl_str_mv 2005-02-28
2005-02-28T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/4143
url http://hdl.handle.net/1822/4143
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lima, C. F., Andrade, P. B., Seabra, R. M., Fernandes-Ferreira, M., & Pereira-Wilson, C. (2005). The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. Journal of Ethnopharmacology, 97(2), 383-389. doi: https://doi.org/10.1016/j.jep.2004.11.029
0378-8741
10.1016/j.jep.2004.11.029
15707779
https://www.sciencedirect.com/science/article/abs/pii/S0378874104005902
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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