Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/10484 |
Resumo: | Essential oils (EOs) obtained from Juniperus communis L. are frequently used in the production of bioproducts. However, there are no studies regarding industrial crops' production, allowing for better control of the quality and production of juniper EOs. To select the plant material for developing future crops of this species in northern Spain, four locations where this shrub species grows in the wild were selected and samples of both genera were collected. The EOs were obtained by steam distillation, and their chemical composition and bioactivity were evaluated. The results showed that the yield of EOs from male and female samples were within the usual reported ranges, varying between 0.24 and 0.58% (dry basis, d.b.). However, limonene content in three locations varied between 15 and 25%, which is between 100% and 200% higher than the values usually reported for other European countries. The antibacterial activity was determined by broth microdilution and showed that gram-positive bacteria were more susceptible to the tested EOs since, in general, lower MIC values were obtained compared to gram-negatives. The EOs from location 1 (L1F) and 2 (L2M) inhibited the growth of six out of the eight clinical strains tested. Samples from location 1 were particularly effective, exhibiting MBC against two gram-negative (E. coli and P. mirabilis) and one gram-positive bacteria (E. faecalis). Moreover, the majority of the EOs tested showed anti-inflammatory activity. Cytotoxic effect has been demonstrated in tumor cell lines, with the best results observed against gastric carcinoma (AGS) cells (GI(50) between 7 to 77 mu g/mL). Although generally presenting higher GI50, most samples also inhibited the growth of non-tumoral cells, particularly hepatocytes (PLP2 cells). Therefore, its use for their anti-proliferative activity should consider specific conditions to avoid damaging normal cells. Finally, the results and conclusions obtained led to the selection of the female shrubs from location 1 (L1F) as the plant material to be propagated in order to produce plants for a future juniper crop. |
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Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish originsDistillationFoliageEssential oilAntioxidant activityCytotoxicityAnti-inflammatory activityEssential oils (EOs) obtained from Juniperus communis L. are frequently used in the production of bioproducts. However, there are no studies regarding industrial crops' production, allowing for better control of the quality and production of juniper EOs. To select the plant material for developing future crops of this species in northern Spain, four locations where this shrub species grows in the wild were selected and samples of both genera were collected. The EOs were obtained by steam distillation, and their chemical composition and bioactivity were evaluated. The results showed that the yield of EOs from male and female samples were within the usual reported ranges, varying between 0.24 and 0.58% (dry basis, d.b.). However, limonene content in three locations varied between 15 and 25%, which is between 100% and 200% higher than the values usually reported for other European countries. The antibacterial activity was determined by broth microdilution and showed that gram-positive bacteria were more susceptible to the tested EOs since, in general, lower MIC values were obtained compared to gram-negatives. The EOs from location 1 (L1F) and 2 (L2M) inhibited the growth of six out of the eight clinical strains tested. Samples from location 1 were particularly effective, exhibiting MBC against two gram-negative (E. coli and P. mirabilis) and one gram-positive bacteria (E. faecalis). Moreover, the majority of the EOs tested showed anti-inflammatory activity. Cytotoxic effect has been demonstrated in tumor cell lines, with the best results observed against gastric carcinoma (AGS) cells (GI(50) between 7 to 77 mu g/mL). Although generally presenting higher GI50, most samples also inhibited the growth of non-tumoral cells, particularly hepatocytes (PLP2 cells). Therefore, its use for their anti-proliferative activity should consider specific conditions to avoid damaging normal cells. Finally, the results and conclusions obtained led to the selection of the female shrubs from location 1 (L1F) as the plant material to be propagated in order to produce plants for a future juniper crop.This research received funding from the Bio Based Industries Joint Undertaking (JU), under grant agreement No 887917 BeonNAT. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio Based Industries Consortium, and the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). L. Barros and R.C. Calhelha thank the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts.MDPIBiblioteca Digital do IPBEsteban, Luis SaúlMediavilla, IreneXavier, VirginieAmaral, Joana S.Pires, Tânia C.S.P.Calhelha, Ricardo C.Lopez, CesarBarros, Lillian2014-09-19T11:38:02Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/10484engEsteban, Luis Saúl; Mediavilla, Irene; Xavier, Virginie; Amaral, Joana S.; Pires, Tânia C.S.P.; Calhelha, Ricardo C.; Lopez, Cesar; Barros, Lillian (2023). Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins. Molecules. eISSN 1420-3049. 28:11, p. 1-1510.3390/molecules281144481420-3049info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T01:18:03Zoai:bibliotecadigital.ipb.pt:10198/10484Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:19:56.141841Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
title |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
spellingShingle |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins Esteban, Luis Saúl Distillation Foliage Essential oil Antioxidant activity Cytotoxicity Anti-inflammatory activity |
title_short |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
title_full |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
title_fullStr |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
title_full_unstemmed |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
title_sort |
Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins |
author |
Esteban, Luis Saúl |
author_facet |
Esteban, Luis Saúl Mediavilla, Irene Xavier, Virginie Amaral, Joana S. Pires, Tânia C.S.P. Calhelha, Ricardo C. Lopez, Cesar Barros, Lillian |
author_role |
author |
author2 |
Mediavilla, Irene Xavier, Virginie Amaral, Joana S. Pires, Tânia C.S.P. Calhelha, Ricardo C. Lopez, Cesar Barros, Lillian |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Esteban, Luis Saúl Mediavilla, Irene Xavier, Virginie Amaral, Joana S. Pires, Tânia C.S.P. Calhelha, Ricardo C. Lopez, Cesar Barros, Lillian |
dc.subject.por.fl_str_mv |
Distillation Foliage Essential oil Antioxidant activity Cytotoxicity Anti-inflammatory activity |
topic |
Distillation Foliage Essential oil Antioxidant activity Cytotoxicity Anti-inflammatory activity |
description |
Essential oils (EOs) obtained from Juniperus communis L. are frequently used in the production of bioproducts. However, there are no studies regarding industrial crops' production, allowing for better control of the quality and production of juniper EOs. To select the plant material for developing future crops of this species in northern Spain, four locations where this shrub species grows in the wild were selected and samples of both genera were collected. The EOs were obtained by steam distillation, and their chemical composition and bioactivity were evaluated. The results showed that the yield of EOs from male and female samples were within the usual reported ranges, varying between 0.24 and 0.58% (dry basis, d.b.). However, limonene content in three locations varied between 15 and 25%, which is between 100% and 200% higher than the values usually reported for other European countries. The antibacterial activity was determined by broth microdilution and showed that gram-positive bacteria were more susceptible to the tested EOs since, in general, lower MIC values were obtained compared to gram-negatives. The EOs from location 1 (L1F) and 2 (L2M) inhibited the growth of six out of the eight clinical strains tested. Samples from location 1 were particularly effective, exhibiting MBC against two gram-negative (E. coli and P. mirabilis) and one gram-positive bacteria (E. faecalis). Moreover, the majority of the EOs tested showed anti-inflammatory activity. Cytotoxic effect has been demonstrated in tumor cell lines, with the best results observed against gastric carcinoma (AGS) cells (GI(50) between 7 to 77 mu g/mL). Although generally presenting higher GI50, most samples also inhibited the growth of non-tumoral cells, particularly hepatocytes (PLP2 cells). Therefore, its use for their anti-proliferative activity should consider specific conditions to avoid damaging normal cells. Finally, the results and conclusions obtained led to the selection of the female shrubs from location 1 (L1F) as the plant material to be propagated in order to produce plants for a future juniper crop. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-19T11:38:02Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/10484 |
url |
http://hdl.handle.net/10198/10484 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Esteban, Luis Saúl; Mediavilla, Irene; Xavier, Virginie; Amaral, Joana S.; Pires, Tânia C.S.P.; Calhelha, Ricardo C.; Lopez, Cesar; Barros, Lillian (2023). Yield, chemical composition and bioactivity of essential oils from common juniper (Juniperus communis L.) from different spanish origins. Molecules. eISSN 1420-3049. 28:11, p. 1-15 10.3390/molecules28114448 1420-3049 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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