PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells

Detalhes bibliográficos
Autor(a) principal: Viceconte, Nikenza
Data de Publicação: 2021
Outros Autores: Loriot, Axelle, Lona Abreu, Patricia, Scheibe, Marion, Fradera Sola, Albert, Butter, Falk, De Smet, Charles, Azzalin, Claus Maria, Arnoult, Nausica, Decottignies, Anabelle
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/54362
Resumo: © 2021 Viceconte et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
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spelling PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cellsPARTERRA interactomeTelomereTelomeric RNA© 2021 Viceconte et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.Telomeric repeat-containing RNA (TERRA) molecules play important roles at telomeres, from heterochromatin regulation to telomerase activity control. In human cells, TERRA is transcribed from subtelomeric promoters located on most chromosome ends and associates with telomeres. The origin of mouse TERRA molecules is, however, unclear, as transcription from the pseudoautosomal PAR locus was recently suggested to account for the vast majority of TERRA in embryonic stem cells (ESC). Here, we confirm the production of TERRA from both the chromosome 18q telomere and the PAR locus in mouse embryonic fibroblasts, ESC, and various mouse cancer and immortalized cell lines, and we identify two novel sources of TERRA on mouse chromosome 2 and X. Using various approaches, we show that PAR-TERRA molecules account for the majority of TERRA transcripts, displaying an increase of two to four orders of magnitude compared to the telomeric 18q transcript. Finally, we present a SILAC-based pull-down screen revealing a large overlap between TERRA-interacting proteins in human and mouse cells, including PRC2 complex subunits, chromatin remodeling factors, DNA replication proteins, Aurora kinases, shelterin complex subunits, Bloom helicase, Coilin, and paraspeckle proteins. Hence, despite originating from distinct genomic regions, mouse and human TERRA are likely to play similar functions in cells.RNA SocietyRepositório da Universidade de LisboaViceconte, NikenzaLoriot, AxelleLona Abreu, PatriciaScheibe, MarionFradera Sola, AlbertButter, FalkDe Smet, CharlesAzzalin, Claus MariaArnoult, NausicaDecottignies, Anabelle2022-09-07T15:01:12Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54362engRNA. 2021 Jan;27(1):106-1211355-838210.1261/rna.076281.1201469-9001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:00:08Zoai:repositorio.ul.pt:10451/54362Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:53.089124Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
title PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
spellingShingle PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
Viceconte, Nikenza
PAR
TERRA interactome
Telomere
Telomeric RNA
title_short PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
title_full PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
title_fullStr PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
title_full_unstemmed PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
title_sort PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells
author Viceconte, Nikenza
author_facet Viceconte, Nikenza
Loriot, Axelle
Lona Abreu, Patricia
Scheibe, Marion
Fradera Sola, Albert
Butter, Falk
De Smet, Charles
Azzalin, Claus Maria
Arnoult, Nausica
Decottignies, Anabelle
author_role author
author2 Loriot, Axelle
Lona Abreu, Patricia
Scheibe, Marion
Fradera Sola, Albert
Butter, Falk
De Smet, Charles
Azzalin, Claus Maria
Arnoult, Nausica
Decottignies, Anabelle
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Viceconte, Nikenza
Loriot, Axelle
Lona Abreu, Patricia
Scheibe, Marion
Fradera Sola, Albert
Butter, Falk
De Smet, Charles
Azzalin, Claus Maria
Arnoult, Nausica
Decottignies, Anabelle
dc.subject.por.fl_str_mv PAR
TERRA interactome
Telomere
Telomeric RNA
topic PAR
TERRA interactome
Telomere
Telomeric RNA
description © 2021 Viceconte et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-09-07T15:01:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/54362
url http://hdl.handle.net/10451/54362
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv RNA. 2021 Jan;27(1):106-121
1355-8382
10.1261/rna.076281.120
1469-9001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv RNA Society
publisher.none.fl_str_mv RNA Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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