Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration

Detalhes bibliográficos
Autor(a) principal: Veiga, Anabela
Data de Publicação: 2022
Outros Autores: Magalhães, Rui, Duarte, Marta M., Dias, Juliana R., Alves, Nuno M., Costa-Pinto, Ana Rita, Castro, Filipa, Rocha, Fernando, Oliveira, Ana L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8368
Resumo: This work was financially supported by: national funds through FCT (Foundation for Science and Technology) under the project UIDB/50016/2020 of the Centre for Biotechnology and Fine Chemistry-CBQF. This work was financially supported by LA/P/0045/2020 (ALiCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); and FCT through the following Projects: UIDB/04044/2020, UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (Nº 022158), MATIS (CENTRO-01-0145-FEDER-000014-3362) and Bone2Move (POCI-01-0145-FEDER-31146). A. Veiga gratefully acknowledges doctoral scholarship [2020.08683.BD] from FCT.
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spelling Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regenerationCalcium phosphate-based materials (CaP)Cerium (Ce)Human dermal fibroblasts (HDFs)Modular oscillatory flow plate reactor (MOPR)Silk sericin (SS)Skin regenerationThis work was financially supported by: national funds through FCT (Foundation for Science and Technology) under the project UIDB/50016/2020 of the Centre for Biotechnology and Fine Chemistry-CBQF. This work was financially supported by LA/P/0045/2020 (ALiCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); and FCT through the following Projects: UIDB/04044/2020, UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (Nº 022158), MATIS (CENTRO-01-0145-FEDER-000014-3362) and Bone2Move (POCI-01-0145-FEDER-31146). A. Veiga gratefully acknowledges doctoral scholarship [2020.08683.BD] from FCT.Calcium plays an important role in barrier function repair and skin homeostasis. In particular, calcium phosphates (CaPs) are well established materials for biomedical engineering due to their biocompatibility. To generate biomaterials with a more complete set of biological properties, previously discarded silk sericin (SS) has been recovered and used as a template to grow CaPs. Crucial characteristics for skin applications, such as antibacterial activity, can be further enhanced by doping CaPs with cerium (Ce) ions. The effectiveness of cell attachment and growth on the materials highly depends on their morphology, particle size distribution, and chemical composition. These characteristics can be tailored through the application of oscillatory flow technology, which provides precise mixing control of the reaction medium. Thus, in the present work, CaP/SS and CaP/SS/Ce particles were fabricated for the first time using a modular oscillatory flow plate reactor (MOFPR) in a continuous mode. Furthermore, the biological behavior of both these composites and of previously produced pure CaPs was assessed using human dermal fibroblasts (HDFs). It was demonstrated that both CaP based with plate-shaped nanoparticles and CaP-SS-based composites significantly improved cell viability and proliferation over time. The results obtained represent a first step towards the reinvention of CaPs for skin engineering.MDPIIC-OnlineVeiga, AnabelaMagalhães, RuiDuarte, Marta M.Dias, Juliana R.Alves, Nuno M.Costa-Pinto, Ana RitaCastro, FilipaRocha, FernandoOliveira, Ana L.2023-04-12T14:23:08Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8368engVeiga, A.; Magalhães, R.; Duarte, M.M.; Dias, J.R.; Alves, N.M.; Costa-Pinto, A.R.; Castro, F.; Rocha, F.; Oliveira, A.L. Continuous Production of Highly Tuned Silk/Calcium-Based Composites: Exploring New Pathways for Skin Regeneration. Molecules 2022, 27, 2249. https://doi.org/10.3390/ molecules270722491420-304910.3390/ molecules27072249info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-05-23T14:53:09ZPortal AgregadorONG
dc.title.none.fl_str_mv Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
title Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
spellingShingle Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
Veiga, Anabela
Calcium phosphate-based materials (CaP)
Cerium (Ce)
Human dermal fibroblasts (HDFs)
Modular oscillatory flow plate reactor (MOPR)
Silk sericin (SS)
Skin regeneration
title_short Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
title_full Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
title_fullStr Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
title_full_unstemmed Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
title_sort Continuous production of highly tuned silk/calcium-based composites: Exploring new pathways for skin regeneration
author Veiga, Anabela
author_facet Veiga, Anabela
Magalhães, Rui
Duarte, Marta M.
Dias, Juliana R.
Alves, Nuno M.
Costa-Pinto, Ana Rita
Castro, Filipa
Rocha, Fernando
Oliveira, Ana L.
author_role author
author2 Magalhães, Rui
Duarte, Marta M.
Dias, Juliana R.
Alves, Nuno M.
Costa-Pinto, Ana Rita
Castro, Filipa
Rocha, Fernando
Oliveira, Ana L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Veiga, Anabela
Magalhães, Rui
Duarte, Marta M.
Dias, Juliana R.
Alves, Nuno M.
Costa-Pinto, Ana Rita
Castro, Filipa
Rocha, Fernando
Oliveira, Ana L.
dc.subject.por.fl_str_mv Calcium phosphate-based materials (CaP)
Cerium (Ce)
Human dermal fibroblasts (HDFs)
Modular oscillatory flow plate reactor (MOPR)
Silk sericin (SS)
Skin regeneration
topic Calcium phosphate-based materials (CaP)
Cerium (Ce)
Human dermal fibroblasts (HDFs)
Modular oscillatory flow plate reactor (MOPR)
Silk sericin (SS)
Skin regeneration
description This work was financially supported by: national funds through FCT (Foundation for Science and Technology) under the project UIDB/50016/2020 of the Centre for Biotechnology and Fine Chemistry-CBQF. This work was financially supported by LA/P/0045/2020 (ALiCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); and FCT through the following Projects: UIDB/04044/2020, UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (Nº 022158), MATIS (CENTRO-01-0145-FEDER-000014-3362) and Bone2Move (POCI-01-0145-FEDER-31146). A. Veiga gratefully acknowledges doctoral scholarship [2020.08683.BD] from FCT.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
2023-04-12T14:23:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8368
url http://hdl.handle.net/10400.8/8368
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veiga, A.; Magalhães, R.; Duarte, M.M.; Dias, J.R.; Alves, N.M.; Costa-Pinto, A.R.; Castro, F.; Rocha, F.; Oliveira, A.L. Continuous Production of Highly Tuned Silk/Calcium-Based Composites: Exploring New Pathways for Skin Regeneration. Molecules 2022, 27, 2249. https://doi.org/10.3390/ molecules27072249
1420-3049
10.3390/ molecules27072249
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.mail.fl_str_mv
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