Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/38122 |
Resumo: | It is essential to design a multifunctional well-controlled platform to transfermechanical cues to the cells in different magnitudes. This study introduces aplatform, a miniaturized bioreactor, which enables to study the effect of shearstress in microsized compartmentalized structures. In this system, thewell-established cell encapsulation system of liquefied capsules (LCs) is usedas microbioreactors in which the encapsulated cells are exposed to variablecore viscosities to experience different mechanical forces under a 3D dynamicculture. The LC technology is joined with electrospraying to produce suchmicrobioreactors at high rates, thus allowing the application of microcapsulesfor high-throughput screening. Using this platform for osteogenicdifferentiation as an example, shows that microbioreactors with higher coreviscosity which produce higher shear stress lead to significantly higherosteogenic characteristics. Moreover, in this system the forces experienced bycells in each LC are simulated by computational modeling. The maximum wallshear stress applied to the cells inside the bioreactor with low, and high coreviscosity environment is estimated to be 297 and 1367 mPa, respectively, forthe experimental setup employed. This work outlines the potential of LCmicrobioreactors as a reliable in vitro customizable platform with a wide rangeof applications. |
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Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitmentMicrobioreactorOsteogenic differentiationDynamic microenvironmentMechanical cuesShear stressIt is essential to design a multifunctional well-controlled platform to transfermechanical cues to the cells in different magnitudes. This study introduces aplatform, a miniaturized bioreactor, which enables to study the effect of shearstress in microsized compartmentalized structures. In this system, thewell-established cell encapsulation system of liquefied capsules (LCs) is usedas microbioreactors in which the encapsulated cells are exposed to variablecore viscosities to experience different mechanical forces under a 3D dynamicculture. The LC technology is joined with electrospraying to produce suchmicrobioreactors at high rates, thus allowing the application of microcapsulesfor high-throughput screening. Using this platform for osteogenicdifferentiation as an example, shows that microbioreactors with higher coreviscosity which produce higher shear stress lead to significantly higherosteogenic characteristics. Moreover, in this system the forces experienced bycells in each LC are simulated by computational modeling. The maximum wallshear stress applied to the cells inside the bioreactor with low, and high coreviscosity environment is estimated to be 297 and 1367 mPa, respectively, forthe experimental setup employed. This work outlines the potential of LCmicrobioreactors as a reliable in vitro customizable platform with a wide rangeof applications.Wiley2023-06-19T09:28:10Z2023-04-08T00:00:00Z2023-04-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://hdl.handle.net/10773/38122eng10.1002/smtd.202201503Ghasemzadeh-Hasankolaei, MaryamMiranda, João M.Correia, Clara R.Mano, João F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:14:28Zoai:ria.ua.pt:10773/38122Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:08:40.324219Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
title |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
spellingShingle |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment Ghasemzadeh-Hasankolaei, Maryam Microbioreactor Osteogenic differentiation Dynamic microenvironment Mechanical cues Shear stress |
title_short |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
title_full |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
title_fullStr |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
title_full_unstemmed |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
title_sort |
Viscous microcapsules as microbioreactors to study mesenchymal stem/stromal cells osteolineage commitment |
author |
Ghasemzadeh-Hasankolaei, Maryam |
author_facet |
Ghasemzadeh-Hasankolaei, Maryam Miranda, João M. Correia, Clara R. Mano, João F. |
author_role |
author |
author2 |
Miranda, João M. Correia, Clara R. Mano, João F. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ghasemzadeh-Hasankolaei, Maryam Miranda, João M. Correia, Clara R. Mano, João F. |
dc.subject.por.fl_str_mv |
Microbioreactor Osteogenic differentiation Dynamic microenvironment Mechanical cues Shear stress |
topic |
Microbioreactor Osteogenic differentiation Dynamic microenvironment Mechanical cues Shear stress |
description |
It is essential to design a multifunctional well-controlled platform to transfermechanical cues to the cells in different magnitudes. This study introduces aplatform, a miniaturized bioreactor, which enables to study the effect of shearstress in microsized compartmentalized structures. In this system, thewell-established cell encapsulation system of liquefied capsules (LCs) is usedas microbioreactors in which the encapsulated cells are exposed to variablecore viscosities to experience different mechanical forces under a 3D dynamicculture. The LC technology is joined with electrospraying to produce suchmicrobioreactors at high rates, thus allowing the application of microcapsulesfor high-throughput screening. Using this platform for osteogenicdifferentiation as an example, shows that microbioreactors with higher coreviscosity which produce higher shear stress lead to significantly higherosteogenic characteristics. Moreover, in this system the forces experienced bycells in each LC are simulated by computational modeling. The maximum wallshear stress applied to the cells inside the bioreactor with low, and high coreviscosity environment is estimated to be 297 and 1367 mPa, respectively, forthe experimental setup employed. This work outlines the potential of LCmicrobioreactors as a reliable in vitro customizable platform with a wide rangeof applications. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-19T09:28:10Z 2023-04-08T00:00:00Z 2023-04-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/38122 |
url |
http://hdl.handle.net/10773/38122 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1002/smtd.202201503 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137738955423744 |