Inhibition of human neutrophil oxidative burst by pyrazolone derivatives

Detalhes bibliográficos
Autor(a) principal: Costa, David
Data de Publicação: 2006
Outros Autores: Marques, A. P., Reis, R. L., Lima, José L. F. C., Fernandes, Eduarda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/20057
Resumo: The risk of agranulocytosis associated with the use of pyrazolone drugs at therapeutical doses and for short periods of time has been considered to be very low. However, little or no attention at all has been devoted to the possible hindrance of neutrophil burst and scavenging of neutrophilgenerated reactive oxygen species (ROS) by these compounds. Such an effect could be beneficial in the case of overactivation of neutrophils but could also be highly detrimental if the number of circulating neutrophils is already decreased. Thus, the aim of the present study was to evaluate the putative inhibitory effect of the pyrazolones dipyrone, aminopyrine, isopropylantipyrine, and antipyrine against human neutrophil burst and their scavenging activity against O2 S!, H2O2, HOS, ROOS, and HOCl. The obtained results showed that dipyrone and aminopyrine prevent phorbol-12-myristate-13-acetate-induced neutrophil burst with high efficiency, while isopropylantipyrine had little effect and antipyrine had no effect at all. Dipyrone and aminopyrine were highly potent scavengers of HOS and HOCl, while, in accordance with the neutrophil burst results, isopropylantipyrine had little effect and antipyrine had no effect at all against these two ROS. None of the studied pyrazolones was capable of scavenging O2 S! or H2O2, while dipyrone was shown to be the most reactive against ROOS.
id RCAP_16fe0465e22746ab39bda88a533698bc
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/20057
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Inhibition of human neutrophil oxidative burst by pyrazolone derivativesHuman neutrophilsOxidative burstMyeloperoxidaseNADPH oxidaseReactive oxygen speciesPyrazolone derivativesDipyroneAminopyrineIsopropylantipyrineAntipyrineFree radicalsScience & TechnologyThe risk of agranulocytosis associated with the use of pyrazolone drugs at therapeutical doses and for short periods of time has been considered to be very low. However, little or no attention at all has been devoted to the possible hindrance of neutrophil burst and scavenging of neutrophilgenerated reactive oxygen species (ROS) by these compounds. Such an effect could be beneficial in the case of overactivation of neutrophils but could also be highly detrimental if the number of circulating neutrophils is already decreased. Thus, the aim of the present study was to evaluate the putative inhibitory effect of the pyrazolones dipyrone, aminopyrine, isopropylantipyrine, and antipyrine against human neutrophil burst and their scavenging activity against O2 S!, H2O2, HOS, ROOS, and HOCl. The obtained results showed that dipyrone and aminopyrine prevent phorbol-12-myristate-13-acetate-induced neutrophil burst with high efficiency, while isopropylantipyrine had little effect and antipyrine had no effect at all. Dipyrone and aminopyrine were highly potent scavengers of HOS and HOCl, while, in accordance with the neutrophil burst results, isopropylantipyrine had little effect and antipyrine had no effect at all against these two ROS. None of the studied pyrazolones was capable of scavenging O2 S! or H2O2, while dipyrone was shown to be the most reactive against ROOS.Elsevier 1Universidade do MinhoCosta, DavidMarques, A. P.Reis, R. L.Lima, José L. F. C.Fernandes, Eduarda20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/20057eng0891-584910.1016/j.freeradbiomed.2005.09.01716458194info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-25T02:07:14Zoai:repositorium.sdum.uminho.pt:1822/20057Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-25T02:07:14Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
title Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
spellingShingle Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
Costa, David
Human neutrophils
Oxidative burst
Myeloperoxidase
NADPH oxidase
Reactive oxygen species
Pyrazolone derivatives
Dipyrone
Aminopyrine
Isopropylantipyrine
Antipyrine
Free radicals
Science & Technology
title_short Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
title_full Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
title_fullStr Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
title_full_unstemmed Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
title_sort Inhibition of human neutrophil oxidative burst by pyrazolone derivatives
author Costa, David
author_facet Costa, David
Marques, A. P.
Reis, R. L.
Lima, José L. F. C.
Fernandes, Eduarda
author_role author
author2 Marques, A. P.
Reis, R. L.
Lima, José L. F. C.
Fernandes, Eduarda
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Costa, David
Marques, A. P.
Reis, R. L.
Lima, José L. F. C.
Fernandes, Eduarda
dc.subject.por.fl_str_mv Human neutrophils
Oxidative burst
Myeloperoxidase
NADPH oxidase
Reactive oxygen species
Pyrazolone derivatives
Dipyrone
Aminopyrine
Isopropylantipyrine
Antipyrine
Free radicals
Science & Technology
topic Human neutrophils
Oxidative burst
Myeloperoxidase
NADPH oxidase
Reactive oxygen species
Pyrazolone derivatives
Dipyrone
Aminopyrine
Isopropylantipyrine
Antipyrine
Free radicals
Science & Technology
description The risk of agranulocytosis associated with the use of pyrazolone drugs at therapeutical doses and for short periods of time has been considered to be very low. However, little or no attention at all has been devoted to the possible hindrance of neutrophil burst and scavenging of neutrophilgenerated reactive oxygen species (ROS) by these compounds. Such an effect could be beneficial in the case of overactivation of neutrophils but could also be highly detrimental if the number of circulating neutrophils is already decreased. Thus, the aim of the present study was to evaluate the putative inhibitory effect of the pyrazolones dipyrone, aminopyrine, isopropylantipyrine, and antipyrine against human neutrophil burst and their scavenging activity against O2 S!, H2O2, HOS, ROOS, and HOCl. The obtained results showed that dipyrone and aminopyrine prevent phorbol-12-myristate-13-acetate-induced neutrophil burst with high efficiency, while isopropylantipyrine had little effect and antipyrine had no effect at all. Dipyrone and aminopyrine were highly potent scavengers of HOS and HOCl, while, in accordance with the neutrophil burst results, isopropylantipyrine had little effect and antipyrine had no effect at all against these two ROS. None of the studied pyrazolones was capable of scavenging O2 S! or H2O2, while dipyrone was shown to be the most reactive against ROOS.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/20057
url https://hdl.handle.net/1822/20057
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0891-5849
10.1016/j.freeradbiomed.2005.09.017
16458194
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier 1
publisher.none.fl_str_mv Elsevier 1
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817545073437442048