Yeast metabolic chassis designs for diverse biotechnological products

Detalhes bibliográficos
Autor(a) principal: Jouhten, Paula
Data de Publicação: 2016
Outros Autores: Boruta, T., Andrejev, S., Pereira, Filipa, Rocha, Isabel, Patil, K. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/43868
Resumo: Supplementary information accompanies this paper at http://www.nature.com/srep
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spelling Yeast metabolic chassis designs for diverse biotechnological productsScience & TechnologySupplementary information accompanies this paper at http://www.nature.com/srepThe diversity of industrially important molecules for which microbial production routes have been experimentally demonstrated is rapidly increasing. The development of economically viable producer cells is, however, lagging behind, as it requires substantial engineering of the host metabolism. A chassis strain suitable for production of a range of molecules is therefore highly sought after but remains elusive. Here, we propose a genome-scale metabolic modeling approach to design chassis strains of Saccharomyces cerevisiae - a widely used microbial cell factory. For a group of 29 products covering a broad range of biochemistry and applications, we identified modular metabolic engineering strategies for re-routing carbon flux towards the desired product. We find distinct product families with shared targets forming the basis for the corresponding chassis cells. The design strategies include overexpression targets that group products by similarity in precursor and cofactor requirements, as well as gene deletion strategies for growth-product coupling that lead to non-intuitive product groups. Our results reveal the extent and the nature of flux re-routing necessary for producing a diverse range of products in a widely used cell factory and provide blueprints for constructing pre-optimized chassis strains.This study was supported by BMBF, Germany (project: DeYeastLibrary, Grant number: 031A343A, ERA-IB-2/003/2013) and FCT, Portugal (Project: DeYeastLibrary, ERA-IB-2/003/2013). We thank A. Zelezniak and E. Valentini for help in drawing the yeast pathway figure, and S. Sheridan for proofreading and comments on the manuscript.Nature Publishing GroupUniversidade do MinhoJouhten, PaulaBoruta, T.Andrejev, S.Pereira, FilipaRocha, IsabelPatil, K. R.2016-07-192016-07-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/43868engJouhten, Paula; Boruta, T.; Andrejev, S.; Pereira, Filipa; Rocha, Isabel; Patil, K. R., Yeast metabolic chassis designs for diverse biotechnological products. Scientific Reports, 6(29694), 1-9, 20162045-23222045-232210.1038/srep2969427430744http://www.nature.com/srep/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:23Zoai:repositorium.sdum.uminho.pt:1822/43868Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:13:30.048393Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Yeast metabolic chassis designs for diverse biotechnological products
title Yeast metabolic chassis designs for diverse biotechnological products
spellingShingle Yeast metabolic chassis designs for diverse biotechnological products
Jouhten, Paula
Science & Technology
title_short Yeast metabolic chassis designs for diverse biotechnological products
title_full Yeast metabolic chassis designs for diverse biotechnological products
title_fullStr Yeast metabolic chassis designs for diverse biotechnological products
title_full_unstemmed Yeast metabolic chassis designs for diverse biotechnological products
title_sort Yeast metabolic chassis designs for diverse biotechnological products
author Jouhten, Paula
author_facet Jouhten, Paula
Boruta, T.
Andrejev, S.
Pereira, Filipa
Rocha, Isabel
Patil, K. R.
author_role author
author2 Boruta, T.
Andrejev, S.
Pereira, Filipa
Rocha, Isabel
Patil, K. R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Jouhten, Paula
Boruta, T.
Andrejev, S.
Pereira, Filipa
Rocha, Isabel
Patil, K. R.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Supplementary information accompanies this paper at http://www.nature.com/srep
publishDate 2016
dc.date.none.fl_str_mv 2016-07-19
2016-07-19T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/43868
url http://hdl.handle.net/1822/43868
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Jouhten, Paula; Boruta, T.; Andrejev, S.; Pereira, Filipa; Rocha, Isabel; Patil, K. R., Yeast metabolic chassis designs for diverse biotechnological products. Scientific Reports, 6(29694), 1-9, 2016
2045-2322
2045-2322
10.1038/srep29694
27430744
http://www.nature.com/srep/index.html
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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