Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/28912 |
Resumo: | Anthracnose caused by Colletotrichum lupini is the most important disease affecting lupin cultivation worldwide. Lupinus mutabilis has been widely studied due to its high protein and oil content. However, it has proved to be sensitive to anthracnose, which limits the expansion of its cultivation. In this work, we seek to unveil the strategy that is used by C. lupini to infect and colonize L. mutabilis tissues using light and transmission electron microscopy (TEM). On petioles, pathogen penetration occurred from melanized appressoria, subcuticular intramural hyphae were seen 2 days after inoculation (dai), and the adjacent host cells remained intact. The switch to necrotrophy was observed 3 dai. At this time, the hyphae extended their colonization to the epidermal, cortex, and vascular cells. Wall degradation was more evident in the epidermal cells. TEM observations also revealed a loss of plasma membrane integrity and different levels of cytoplasm disorganization in the infected epidermal cells and in those of the first layers of the cortex. The disintegration of organelles occurred and was particularly visible in the chloroplasts. The necrotrophic phase culminated with the development of acervuli 6 dai. C. lupini used the same infection strategy on stems, but there was a delay in the penetration of host tissues and the appearance of the first symptoms |
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Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilislupins anthracnosefungal infection strategyhistologyultrastructureAnthracnose caused by Colletotrichum lupini is the most important disease affecting lupin cultivation worldwide. Lupinus mutabilis has been widely studied due to its high protein and oil content. However, it has proved to be sensitive to anthracnose, which limits the expansion of its cultivation. In this work, we seek to unveil the strategy that is used by C. lupini to infect and colonize L. mutabilis tissues using light and transmission electron microscopy (TEM). On petioles, pathogen penetration occurred from melanized appressoria, subcuticular intramural hyphae were seen 2 days after inoculation (dai), and the adjacent host cells remained intact. The switch to necrotrophy was observed 3 dai. At this time, the hyphae extended their colonization to the epidermal, cortex, and vascular cells. Wall degradation was more evident in the epidermal cells. TEM observations also revealed a loss of plasma membrane integrity and different levels of cytoplasm disorganization in the infected epidermal cells and in those of the first layers of the cortex. The disintegration of organelles occurred and was particularly visible in the chloroplasts. The necrotrophic phase culminated with the development of acervuli 6 dai. C. lupini used the same infection strategy on stems, but there was a delay in the penetration of host tissues and the appearance of the first symptomsMDPIRepositório da Universidade de LisboaGuilengue, NorbertoSilva, Maria do CéuTalhinhas, PedroNeves-Martins, JoãoLoureiro, Andreia2023-10-09T10:33:54Z2022-112022-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/28912engGuilengue, N.; Silva, M.d.C.; Talhinhas, P.; Neves-Martins, J.; Loureiro, A. Subcuticular– Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis. Plants 2022, 11, 302810.3390/plants11223028info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-15T01:32:49Zoai:www.repository.utl.pt:10400.5/28912Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:35:42.295131Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
title |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
spellingShingle |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis Guilengue, Norberto lupins anthracnose fungal infection strategy histology ultrastructure |
title_short |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
title_full |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
title_fullStr |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
title_full_unstemmed |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
title_sort |
Subcuticular–Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis |
author |
Guilengue, Norberto |
author_facet |
Guilengue, Norberto Silva, Maria do Céu Talhinhas, Pedro Neves-Martins, João Loureiro, Andreia |
author_role |
author |
author2 |
Silva, Maria do Céu Talhinhas, Pedro Neves-Martins, João Loureiro, Andreia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Guilengue, Norberto Silva, Maria do Céu Talhinhas, Pedro Neves-Martins, João Loureiro, Andreia |
dc.subject.por.fl_str_mv |
lupins anthracnose fungal infection strategy histology ultrastructure |
topic |
lupins anthracnose fungal infection strategy histology ultrastructure |
description |
Anthracnose caused by Colletotrichum lupini is the most important disease affecting lupin cultivation worldwide. Lupinus mutabilis has been widely studied due to its high protein and oil content. However, it has proved to be sensitive to anthracnose, which limits the expansion of its cultivation. In this work, we seek to unveil the strategy that is used by C. lupini to infect and colonize L. mutabilis tissues using light and transmission electron microscopy (TEM). On petioles, pathogen penetration occurred from melanized appressoria, subcuticular intramural hyphae were seen 2 days after inoculation (dai), and the adjacent host cells remained intact. The switch to necrotrophy was observed 3 dai. At this time, the hyphae extended their colonization to the epidermal, cortex, and vascular cells. Wall degradation was more evident in the epidermal cells. TEM observations also revealed a loss of plasma membrane integrity and different levels of cytoplasm disorganization in the infected epidermal cells and in those of the first layers of the cortex. The disintegration of organelles occurred and was particularly visible in the chloroplasts. The necrotrophic phase culminated with the development of acervuli 6 dai. C. lupini used the same infection strategy on stems, but there was a delay in the penetration of host tissues and the appearance of the first symptoms |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11 2022-11-01T00:00:00Z 2023-10-09T10:33:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/28912 |
url |
http://hdl.handle.net/10400.5/28912 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Guilengue, N.; Silva, M.d.C.; Talhinhas, P.; Neves-Martins, J.; Loureiro, A. Subcuticular– Intracellular Hemibiotrophy of Colletotrichum lupini in Lupinus mutabilis. Plants 2022, 11, 3028 10.3390/plants11223028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133619985317888 |