Settleable dust and bioburden in Portuguese dwellings

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2020
Outros Autores: Dias, Marta, Almeida, Beatriz, Vicente, Estela, Aranha Caetano, Liliana, Carolino, Elisabete, Alves, Célia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/12414
Resumo: UIDB/05608/2020. UIDP/05608/2020.
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spelling Settleable dust and bioburden in Portuguese dwellingsIndoor airAir qualityPassive sampling methodsSettleable dustBioburdenAzole-resistance screeningAspergillus sp.UIDB/05608/2020UIDP/05608/2020PortugalUIDB/05608/2020. UIDP/05608/2020.Monitoring campaigns in several buildings have shown that occupants exposed to contaminated indoor air generally exhibit diverse health symptoms. This study intends to assess settleable dust loading rates and bioburden in Portuguese dwellings by passive sampling onto quartz fiber filters and electrostatic dust cloths (EDCs), respectively. Settled dust collected by EDCs was analyzed by culture-based methods (including azole-resistance screening) and qPCR, targeting four different toxigenic Aspergillus sections (Flavi, Fumigati, Circumdati, and Nidulantes). Dust loading rates and bioburden showed higher variability in the summer season. In both seasons, Penicillium sp. was the one with the highest prevalence (59.1% winter; 58.1% summer), followed by Aspergillus sp. in winter (13.0%). Fungal contamination increased in the winter period, while bacterial counts decreased. Aspergillus sections Circumdati and Nidulantes, detected in voriconazole supplemented media, and Aspergillus sections Fumigati and Nidulantes, detected by molecular tools, were found in the winter samples. This study reinforces the importance of applying: (a) Passive sampling methods in campaigns in dwellings; (b) two different culture media (MEA and DG18) to assess fungi; (c) in parallel, molecular tools targeting the most suitable indicators of fungal contamination; and (d) azole resistance screening to unveil azole resistance detection in fungal species.MDPIRCIPLViegas, CarlaDias, MartaAlmeida, BeatrizVicente, EstelaAranha Caetano, LilianaCarolino, ElisabeteAlves, Célia2020-12-05T21:56:08Z2020-112020-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/12414engViegas C, Dias M, Almeida B, Vicente E, Caetano LA, Carolino E, et al. Settleable dust and bioburden in Portuguese dwellings. Microorganisms. 2020;8(11):1799.10.3390/microorganisms8111799info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:05:17Zoai:repositorio.ipl.pt:10400.21/12414Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:20:30.428786Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Settleable dust and bioburden in Portuguese dwellings
title Settleable dust and bioburden in Portuguese dwellings
spellingShingle Settleable dust and bioburden in Portuguese dwellings
Viegas, Carla
Indoor air
Air quality
Passive sampling methods
Settleable dust
Bioburden
Azole-resistance screening
Aspergillus sp.
UIDB/05608/2020
UIDP/05608/2020
Portugal
title_short Settleable dust and bioburden in Portuguese dwellings
title_full Settleable dust and bioburden in Portuguese dwellings
title_fullStr Settleable dust and bioburden in Portuguese dwellings
title_full_unstemmed Settleable dust and bioburden in Portuguese dwellings
title_sort Settleable dust and bioburden in Portuguese dwellings
author Viegas, Carla
author_facet Viegas, Carla
Dias, Marta
Almeida, Beatriz
Vicente, Estela
Aranha Caetano, Liliana
Carolino, Elisabete
Alves, Célia
author_role author
author2 Dias, Marta
Almeida, Beatriz
Vicente, Estela
Aranha Caetano, Liliana
Carolino, Elisabete
Alves, Célia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Dias, Marta
Almeida, Beatriz
Vicente, Estela
Aranha Caetano, Liliana
Carolino, Elisabete
Alves, Célia
dc.subject.por.fl_str_mv Indoor air
Air quality
Passive sampling methods
Settleable dust
Bioburden
Azole-resistance screening
Aspergillus sp.
UIDB/05608/2020
UIDP/05608/2020
Portugal
topic Indoor air
Air quality
Passive sampling methods
Settleable dust
Bioburden
Azole-resistance screening
Aspergillus sp.
UIDB/05608/2020
UIDP/05608/2020
Portugal
description UIDB/05608/2020. UIDP/05608/2020.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-05T21:56:08Z
2020-11
2020-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/12414
url http://hdl.handle.net/10400.21/12414
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Dias M, Almeida B, Vicente E, Caetano LA, Carolino E, et al. Settleable dust and bioburden in Portuguese dwellings. Microorganisms. 2020;8(11):1799.
10.3390/microorganisms8111799
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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