Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain

Detalhes bibliográficos
Autor(a) principal: Luz, LL
Data de Publicação: 2015
Outros Autores: Fernandes, EC, Sivado, M, Kokai, E, Szucs, P, Safronov, BV
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10216/114497
Resumo: Referred pain is a phenomenon of feeling pain at a site other than the site of the painful stimulus origin. It arises from a pathological mixing of nociceptive processing pathways for visceral and somatic inputs. Despite numerous studies based on unit recordings from spinal and supraspinal neurons, the exact mechanism and site of this mixing within the central nervous system are not known. Here, we selectively recorded from lamina I neurons, using a visually guided patch-clamp technique, in thoracic spinal cord preparation with preserved intercostal (somatic) and splanchnic (visceral) nerves. We show that somatic and visceral C fibers converge monosynaptically onto a group of lamina I neurons, which includes both projection and local circuit neurons. Other groups of lamina I neurons received inputs from either somatic or visceral afferents. We have also identified a population of lamina I local circuit neurons showing overall inhibitory responses upon stimulation of both nerves. Thus, the present data allow us to draw two major conclusions. First, lamina I of the spinal cord is the first site in the central nervous system where somatic and visceral pathways directly converge onto individual projection and local circuit neurons. Second, the mechanism of somatovisceral convergence is complex and based on functional integration of monosynaptic and polysynaptic excitatory as well as inhibitory inputs in specific groups of neurons. This complex pattern of convergence provides a substrate for alterations in the balance between visceral and somatic inputs causing referred pain.
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spelling Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred painAnimalsBiophysicsElectric StimulationExcitatory Postsynaptic Potentials/physiologyLysine/analogs & derivativesLysine/metabolismNerve Fibers, Unmyelinated/physiologyNeural Conduction/physiologyNeurons/physiologyPain, Referred/pathologyRatsRats, WistarSpinal Cord Dorsal Horn/pathologySplanchnic Nerves/physiopathologySynapses/physiologyVisceral Afferents/physiopathologyReferred pain is a phenomenon of feeling pain at a site other than the site of the painful stimulus origin. It arises from a pathological mixing of nociceptive processing pathways for visceral and somatic inputs. Despite numerous studies based on unit recordings from spinal and supraspinal neurons, the exact mechanism and site of this mixing within the central nervous system are not known. Here, we selectively recorded from lamina I neurons, using a visually guided patch-clamp technique, in thoracic spinal cord preparation with preserved intercostal (somatic) and splanchnic (visceral) nerves. We show that somatic and visceral C fibers converge monosynaptically onto a group of lamina I neurons, which includes both projection and local circuit neurons. Other groups of lamina I neurons received inputs from either somatic or visceral afferents. We have also identified a population of lamina I local circuit neurons showing overall inhibitory responses upon stimulation of both nerves. Thus, the present data allow us to draw two major conclusions. First, lamina I of the spinal cord is the first site in the central nervous system where somatic and visceral pathways directly converge onto individual projection and local circuit neurons. Second, the mechanism of somatovisceral convergence is complex and based on functional integration of monosynaptic and polysynaptic excitatory as well as inhibitory inputs in specific groups of neurons. This complex pattern of convergence provides a substrate for alterations in the balance between visceral and somatic inputs causing referred pain.Lippincott Williams & Wilkins20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10216/114497eng0304-395910.1097/j.pain.0000000000000267Luz, LLFernandes, ECSivado, MKokai, ESzucs, PSafronov, BVinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:43:06Zoai:repositorio-aberto.up.pt:10216/114497Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:46:28.680322Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
title Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
spellingShingle Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
Luz, LL
Animals
Biophysics
Electric Stimulation
Excitatory Postsynaptic Potentials/physiology
Lysine/analogs & derivatives
Lysine/metabolism
Nerve Fibers, Unmyelinated/physiology
Neural Conduction/physiology
Neurons/physiology
Pain, Referred/pathology
Rats
Rats, Wistar
Spinal Cord Dorsal Horn/pathology
Splanchnic Nerves/physiopathology
Synapses/physiology
Visceral Afferents/physiopathology
title_short Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
title_full Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
title_fullStr Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
title_full_unstemmed Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
title_sort Monosynaptic convergence of somatic and visceral C-fiber afferents on projection and local circuit neurons in lamina I: a substrate for referred pain
author Luz, LL
author_facet Luz, LL
Fernandes, EC
Sivado, M
Kokai, E
Szucs, P
Safronov, BV
author_role author
author2 Fernandes, EC
Sivado, M
Kokai, E
Szucs, P
Safronov, BV
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Luz, LL
Fernandes, EC
Sivado, M
Kokai, E
Szucs, P
Safronov, BV
dc.subject.por.fl_str_mv Animals
Biophysics
Electric Stimulation
Excitatory Postsynaptic Potentials/physiology
Lysine/analogs & derivatives
Lysine/metabolism
Nerve Fibers, Unmyelinated/physiology
Neural Conduction/physiology
Neurons/physiology
Pain, Referred/pathology
Rats
Rats, Wistar
Spinal Cord Dorsal Horn/pathology
Splanchnic Nerves/physiopathology
Synapses/physiology
Visceral Afferents/physiopathology
topic Animals
Biophysics
Electric Stimulation
Excitatory Postsynaptic Potentials/physiology
Lysine/analogs & derivatives
Lysine/metabolism
Nerve Fibers, Unmyelinated/physiology
Neural Conduction/physiology
Neurons/physiology
Pain, Referred/pathology
Rats
Rats, Wistar
Spinal Cord Dorsal Horn/pathology
Splanchnic Nerves/physiopathology
Synapses/physiology
Visceral Afferents/physiopathology
description Referred pain is a phenomenon of feeling pain at a site other than the site of the painful stimulus origin. It arises from a pathological mixing of nociceptive processing pathways for visceral and somatic inputs. Despite numerous studies based on unit recordings from spinal and supraspinal neurons, the exact mechanism and site of this mixing within the central nervous system are not known. Here, we selectively recorded from lamina I neurons, using a visually guided patch-clamp technique, in thoracic spinal cord preparation with preserved intercostal (somatic) and splanchnic (visceral) nerves. We show that somatic and visceral C fibers converge monosynaptically onto a group of lamina I neurons, which includes both projection and local circuit neurons. Other groups of lamina I neurons received inputs from either somatic or visceral afferents. We have also identified a population of lamina I local circuit neurons showing overall inhibitory responses upon stimulation of both nerves. Thus, the present data allow us to draw two major conclusions. First, lamina I of the spinal cord is the first site in the central nervous system where somatic and visceral pathways directly converge onto individual projection and local circuit neurons. Second, the mechanism of somatovisceral convergence is complex and based on functional integration of monosynaptic and polysynaptic excitatory as well as inhibitory inputs in specific groups of neurons. This complex pattern of convergence provides a substrate for alterations in the balance between visceral and somatic inputs causing referred pain.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10216/114497
url http://hdl.handle.net/10216/114497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0304-3959
10.1097/j.pain.0000000000000267
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins
publisher.none.fl_str_mv Lippincott Williams & Wilkins
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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