Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/39484 |
Resumo: | Thiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by Labrys portucalensis F11. After 30 days of incubation in mineral salt medium, L. portucalensis F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L−1) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L−1); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using Aliivibrio fischeri and by assessing the synthesis of an inducible β-galactosidase by an E. coli mutant (Toxi-Chromo test). L. portucalensis F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies. |
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Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11Labrys portucalensis F11BiodegradationMetabolitesThiamethoxamToxicityThiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by Labrys portucalensis F11. After 30 days of incubation in mineral salt medium, L. portucalensis F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L−1) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L−1); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using Aliivibrio fischeri and by assessing the synthesis of an inducible β-galactosidase by an E. coli mutant (Toxi-Chromo test). L. portucalensis F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies.Veritati - Repositório Institucional da Universidade Católica PortuguesaBoufercha, OumeimaMonforte, Ana R.Boudemagh, AllaoueddineFerreira, António C.Castro, Paula M. L.Moreira, Irina S.2022-12-06T12:58:08Z2022-11-182022-11-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/39484eng1661-659610.3390/ijms23221432685142787446PMC969441336430799000887299900001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-09T01:36:34Zoai:repositorio.ucp.pt:10400.14/39484Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:32:19.220607Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
title |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
spellingShingle |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 Boufercha, Oumeima Labrys portucalensis F11 Biodegradation Metabolites Thiamethoxam Toxicity |
title_short |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
title_full |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
title_fullStr |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
title_full_unstemmed |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
title_sort |
Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11 |
author |
Boufercha, Oumeima |
author_facet |
Boufercha, Oumeima Monforte, Ana R. Boudemagh, Allaoueddine Ferreira, António C. Castro, Paula M. L. Moreira, Irina S. |
author_role |
author |
author2 |
Monforte, Ana R. Boudemagh, Allaoueddine Ferreira, António C. Castro, Paula M. L. Moreira, Irina S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Boufercha, Oumeima Monforte, Ana R. Boudemagh, Allaoueddine Ferreira, António C. Castro, Paula M. L. Moreira, Irina S. |
dc.subject.por.fl_str_mv |
Labrys portucalensis F11 Biodegradation Metabolites Thiamethoxam Toxicity |
topic |
Labrys portucalensis F11 Biodegradation Metabolites Thiamethoxam Toxicity |
description |
Thiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by Labrys portucalensis F11. After 30 days of incubation in mineral salt medium, L. portucalensis F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L−1) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L−1); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using Aliivibrio fischeri and by assessing the synthesis of an inducible β-galactosidase by an E. coli mutant (Toxi-Chromo test). L. portucalensis F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-06T12:58:08Z 2022-11-18 2022-11-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/39484 |
url |
http://hdl.handle.net/10400.14/39484 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1661-6596 10.3390/ijms232214326 85142787446 PMC9694413 36430799 000887299900001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132047241904128 |