PACAP system evolution and its role in melanophore function in teleost fish skin

Detalhes bibliográficos
Autor(a) principal: CR Cardoso, Joao
Data de Publicação: 2015
Outros Autores: C. Félix, Rute, Martins, Rute, M., Trindade, G Fonseca, Vera, Fuentes, Xoan, Power, Deborah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/14563
Resumo: Pituitary adenylate cyclase-activating polypeptide (PACAP) administered to tilapia melanophores ex-vivo causes significant pigment aggregation and this is a newly identified function for this peptide in fish. The G-protein coupled receptors (GPCRs), adcyap1r1a (encoding Pac1a) and vipr2a (encoding Vpac2a), are the only receptors in melanophores with appreciable levels of expression and are significantly (p < 0.05) down-regulated in the absence of light. Vpac2a is activated exclusively by peptide histidine isoleucine (PHI), which suggests that Pac1a mediates the melanin aggregating effect of PACAP on melanophores. Paradoxically activation of Pac1a with PACAP caused a rise in cAMP, which in fish melanophores is associated with melanin dispersion. We hypothesise that the duplicate adcyap1ra and vipr2a genes in teleosts have acquired a specific role in skin and that the melanin aggregating effect of PACAP results from the interaction of Pac1a with Ramp that attenuates cAMP-dependent PKA activity and favours the Ca(2+)/Calmodulin dependent pathway.
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spelling PACAP system evolution and its role in melanophore function in teleost fish skinAnimalsBiological evolutionDatabasesMelanophoresPhylogenyPituitary Adenylate Cyclase-Activating Polypeptide (PACAP)SkinTilapiaFactualPituitary adenylate cyclase-activating polypeptide (PACAP) administered to tilapia melanophores ex-vivo causes significant pigment aggregation and this is a newly identified function for this peptide in fish. The G-protein coupled receptors (GPCRs), adcyap1r1a (encoding Pac1a) and vipr2a (encoding Vpac2a), are the only receptors in melanophores with appreciable levels of expression and are significantly (p < 0.05) down-regulated in the absence of light. Vpac2a is activated exclusively by peptide histidine isoleucine (PHI), which suggests that Pac1a mediates the melanin aggregating effect of PACAP on melanophores. Paradoxically activation of Pac1a with PACAP caused a rise in cAMP, which in fish melanophores is associated with melanin dispersion. We hypothesise that the duplicate adcyap1ra and vipr2a genes in teleosts have acquired a specific role in skin and that the melanin aggregating effect of PACAP results from the interaction of Pac1a with Ramp that attenuates cAMP-dependent PKA activity and favours the Ca(2+)/Calmodulin dependent pathway.ElsevierSapientiaCR Cardoso, JoaoC. Félix, RuteMartins, RuteM., TrindadeG Fonseca, VeraFuentes, XoanPower, Deborah2020-07-31T14:16:59Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14563eng0303-720710.1016/j.mce.2015.04.020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:26:52Zoai:sapientia.ualg.pt:10400.1/14563Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:35.013859Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv PACAP system evolution and its role in melanophore function in teleost fish skin
title PACAP system evolution and its role in melanophore function in teleost fish skin
spellingShingle PACAP system evolution and its role in melanophore function in teleost fish skin
CR Cardoso, Joao
Animals
Biological evolution
Databases
Melanophores
Phylogeny
Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)
Skin
Tilapia
Factual
title_short PACAP system evolution and its role in melanophore function in teleost fish skin
title_full PACAP system evolution and its role in melanophore function in teleost fish skin
title_fullStr PACAP system evolution and its role in melanophore function in teleost fish skin
title_full_unstemmed PACAP system evolution and its role in melanophore function in teleost fish skin
title_sort PACAP system evolution and its role in melanophore function in teleost fish skin
author CR Cardoso, Joao
author_facet CR Cardoso, Joao
C. Félix, Rute
Martins, Rute
M., Trindade
G Fonseca, Vera
Fuentes, Xoan
Power, Deborah
author_role author
author2 C. Félix, Rute
Martins, Rute
M., Trindade
G Fonseca, Vera
Fuentes, Xoan
Power, Deborah
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv CR Cardoso, Joao
C. Félix, Rute
Martins, Rute
M., Trindade
G Fonseca, Vera
Fuentes, Xoan
Power, Deborah
dc.subject.por.fl_str_mv Animals
Biological evolution
Databases
Melanophores
Phylogeny
Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)
Skin
Tilapia
Factual
topic Animals
Biological evolution
Databases
Melanophores
Phylogeny
Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)
Skin
Tilapia
Factual
description Pituitary adenylate cyclase-activating polypeptide (PACAP) administered to tilapia melanophores ex-vivo causes significant pigment aggregation and this is a newly identified function for this peptide in fish. The G-protein coupled receptors (GPCRs), adcyap1r1a (encoding Pac1a) and vipr2a (encoding Vpac2a), are the only receptors in melanophores with appreciable levels of expression and are significantly (p < 0.05) down-regulated in the absence of light. Vpac2a is activated exclusively by peptide histidine isoleucine (PHI), which suggests that Pac1a mediates the melanin aggregating effect of PACAP on melanophores. Paradoxically activation of Pac1a with PACAP caused a rise in cAMP, which in fish melanophores is associated with melanin dispersion. We hypothesise that the duplicate adcyap1ra and vipr2a genes in teleosts have acquired a specific role in skin and that the melanin aggregating effect of PACAP results from the interaction of Pac1a with Ramp that attenuates cAMP-dependent PKA activity and favours the Ca(2+)/Calmodulin dependent pathway.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2020-07-31T14:16:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/14563
url http://hdl.handle.net/10400.1/14563
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0303-7207
10.1016/j.mce.2015.04.020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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