A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31746 |
Resumo: | Molecular biology techniques provide valuable insights in the investigation of microbial dynamics and evolution. Denaturing gradient gel electrophoresis (DGGE) analysis is one of the most popular methods which have been used in bioprocess assessment. Most of the anaerobic digestion models consider several microbial populations as state variables. However, the difficulty to measure individual species concentrations may cause inaccurate model predictions. The integration of microbial data and ecosystem modelling is currently a challenging issue for improved system control. A novel procedure that combines common experimental measurements, DGGE, and image analysis is presented in this study in order to provide a preliminary estimation of the actual concentration of the dominant bacterial ribotypes in a bioreactor, for further use as variable in mathematical modelling of the bioprocess. This approach was applied during the start-up of a continuous anaerobic bioreactor for hydrogen production. The experimental concentration data were used for determining the kinetic parameters of each species, by using a multi-species chemostat-model. The model was able to reproduce the global trend of substrate and biomass concentrations during the reactor start-up, and predicted in an acceptable way the evolution of each ribotype concentration, depicting properly specific ribotype selection and extinction. |
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A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen productionAnaerobic digestionDGGEFingerprintImage analysisModemodelScience & TechnologyMolecular biology techniques provide valuable insights in the investigation of microbial dynamics and evolution. Denaturing gradient gel electrophoresis (DGGE) analysis is one of the most popular methods which have been used in bioprocess assessment. Most of the anaerobic digestion models consider several microbial populations as state variables. However, the difficulty to measure individual species concentrations may cause inaccurate model predictions. The integration of microbial data and ecosystem modelling is currently a challenging issue for improved system control. A novel procedure that combines common experimental measurements, DGGE, and image analysis is presented in this study in order to provide a preliminary estimation of the actual concentration of the dominant bacterial ribotypes in a bioreactor, for further use as variable in mathematical modelling of the bioprocess. This approach was applied during the start-up of a continuous anaerobic bioreactor for hydrogen production. The experimental concentration data were used for determining the kinetic parameters of each species, by using a multi-species chemostat-model. The model was able to reproduce the global trend of substrate and biomass concentrations during the reactor start-up, and predicted in an acceptable way the evolution of each ribotype concentration, depicting properly specific ribotype selection and extinction.International Water Association Publishing (IWAP)Universidade do MinhoTapia, EstelaDonoso-Bravo, AndresCabrol, LéaAlves, M. M.Pereira, M. A.Rapaport, AlainRuiz-Filippi, Gonzalo20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31746eng0273-12230273-122310.2166/wst.2013.71924552721info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:10Zoai:repositorium.sdum.uminho.pt:1822/31746Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:58.122671Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
title |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
spellingShingle |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production Tapia, Estela Anaerobic digestion DGGE Fingerprint Image analysis Mode model Science & Technology |
title_short |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
title_full |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
title_fullStr |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
title_full_unstemmed |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
title_sort |
A methodology for a quantitative interpretation of DGGE with the help of mathematical modelling: application in bio-hydrogen production |
author |
Tapia, Estela |
author_facet |
Tapia, Estela Donoso-Bravo, Andres Cabrol, Léa Alves, M. M. Pereira, M. A. Rapaport, Alain Ruiz-Filippi, Gonzalo |
author_role |
author |
author2 |
Donoso-Bravo, Andres Cabrol, Léa Alves, M. M. Pereira, M. A. Rapaport, Alain Ruiz-Filippi, Gonzalo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Tapia, Estela Donoso-Bravo, Andres Cabrol, Léa Alves, M. M. Pereira, M. A. Rapaport, Alain Ruiz-Filippi, Gonzalo |
dc.subject.por.fl_str_mv |
Anaerobic digestion DGGE Fingerprint Image analysis Mode model Science & Technology |
topic |
Anaerobic digestion DGGE Fingerprint Image analysis Mode model Science & Technology |
description |
Molecular biology techniques provide valuable insights in the investigation of microbial dynamics and evolution. Denaturing gradient gel electrophoresis (DGGE) analysis is one of the most popular methods which have been used in bioprocess assessment. Most of the anaerobic digestion models consider several microbial populations as state variables. However, the difficulty to measure individual species concentrations may cause inaccurate model predictions. The integration of microbial data and ecosystem modelling is currently a challenging issue for improved system control. A novel procedure that combines common experimental measurements, DGGE, and image analysis is presented in this study in order to provide a preliminary estimation of the actual concentration of the dominant bacterial ribotypes in a bioreactor, for further use as variable in mathematical modelling of the bioprocess. This approach was applied during the start-up of a continuous anaerobic bioreactor for hydrogen production. The experimental concentration data were used for determining the kinetic parameters of each species, by using a multi-species chemostat-model. The model was able to reproduce the global trend of substrate and biomass concentrations during the reactor start-up, and predicted in an acceptable way the evolution of each ribotype concentration, depicting properly specific ribotype selection and extinction. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31746 |
url |
http://hdl.handle.net/1822/31746 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0273-1223 0273-1223 10.2166/wst.2013.719 24552721 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Water Association Publishing (IWAP) |
publisher.none.fl_str_mv |
International Water Association Publishing (IWAP) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799132985263390720 |