Open-source tool for real-time and automated analysis of droplet-based microfluidic
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/158155 |
Resumo: | Publisher Copyright: © 2023 The Royal Society of Chemistry. |
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7160 |
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Open-source tool for real-time and automated analysis of droplet-based microfluidicBioengineeringBiochemistryChemistry(all)Biomedical EngineeringPublisher Copyright: © 2023 The Royal Society of Chemistry.Droplet-based microfluidic technology is a powerful tool for generating large numbers of monodispersed nanoliter-sized droplets for ultra-high throughput screening of molecules or single cells. Yet further progress in the development of methods for the real-time detection and measurement of passing droplets is needed for achieving fully automated systems and ultimately scalability. Existing droplet monitoring technologies are either difficult to implement by non-experts or require complex experimentation setups. Moreover, commercially available monitoring equipment is expensive and therefore limited to a few laboratories worldwide. In this work, we validated for the first time an easy-to-use, open-source Bonsai visual programming language to accurately measure in real-time droplets generated in a microfluidic device. With this method, droplets are found and characterized from bright-field images with high processing speed. We used off-the-shelf components to achieve an optical system that allows sensitive image-based, label-free, and cost-effective monitoring. As a test of its use we present the results, in terms of droplet radius, circulation speed and production frequency, of our method and compared its performance with that of the widely-used ImageJ software. Moreover, we show that similar results are obtained regardless of the degree of expertise. Finally, our goal is to provide a robust, simple to integrate, and user-friendly tool for monitoring droplets, capable of helping researchers to get started in the laboratory immediately, even without programming experience, enabling analysis and reporting of droplet data in real-time and closed-loop experiments.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)UNINOVA-Instituto de Desenvolvimento de Novas TecnologiasDCM - Departamento de Ciência dos MateriaisUCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNNeto, Joana P.Mota, AnaLopes, GonçaloCoelho, Beatriz J.Frazão, JoãoMoura, André T.Oliveira, BeatrizSieira, BárbaraFernandes, JoséFortunato, ElviraMartins, RodrigoIgreja, RuiBaptista, Pedro V.Águas, Hugo2023-09-22T22:16:03Z2023-06-142023-06-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://hdl.handle.net/10362/158155eng1473-0197PURE: 72011333https://doi.org/10.1039/d3lc00327binfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:26Zoai:run.unl.pt:10362/158155Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:00.698793Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| title |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| spellingShingle |
Open-source tool for real-time and automated analysis of droplet-based microfluidic Neto, Joana P. Bioengineering Biochemistry Chemistry(all) Biomedical Engineering |
| title_short |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| title_full |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| title_fullStr |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| title_full_unstemmed |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| title_sort |
Open-source tool for real-time and automated analysis of droplet-based microfluidic |
| author |
Neto, Joana P. |
| author_facet |
Neto, Joana P. Mota, Ana Lopes, Gonçalo Coelho, Beatriz J. Frazão, João Moura, André T. Oliveira, Beatriz Sieira, Bárbara Fernandes, José Fortunato, Elvira Martins, Rodrigo Igreja, Rui Baptista, Pedro V. Águas, Hugo |
| author_role |
author |
| author2 |
Mota, Ana Lopes, Gonçalo Coelho, Beatriz J. Frazão, João Moura, André T. Oliveira, Beatriz Sieira, Bárbara Fernandes, José Fortunato, Elvira Martins, Rodrigo Igreja, Rui Baptista, Pedro V. Águas, Hugo |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias DCM - Departamento de Ciência dos Materiais UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida RUN |
| dc.contributor.author.fl_str_mv |
Neto, Joana P. Mota, Ana Lopes, Gonçalo Coelho, Beatriz J. Frazão, João Moura, André T. Oliveira, Beatriz Sieira, Bárbara Fernandes, José Fortunato, Elvira Martins, Rodrigo Igreja, Rui Baptista, Pedro V. Águas, Hugo |
| dc.subject.por.fl_str_mv |
Bioengineering Biochemistry Chemistry(all) Biomedical Engineering |
| topic |
Bioengineering Biochemistry Chemistry(all) Biomedical Engineering |
| description |
Publisher Copyright: © 2023 The Royal Society of Chemistry. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-09-22T22:16:03Z 2023-06-14 2023-06-14T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/158155 |
| url |
http://hdl.handle.net/10362/158155 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1473-0197 PURE: 72011333 https://doi.org/10.1039/d3lc00327b |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
7 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
| institution |
RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138153735389184 |