NO-mediated apoptosis in yeast

Detalhes bibliográficos
Autor(a) principal: Almeida, Bruno
Data de Publicação: 2007
Outros Autores: Buttner, Sabrina, Ohlmeier, Steffen, Silva, Alexandra, Mesquita, Ana, Marques, Maria Belém Sousa Sampaio, Osório, Nuno S., Kollau, Alexander, Mayer, Bernhard, Leão, Cecília, Laranjinha, João, Rodrigues, Fernando José dos Santos, Madeo, Frank, Ludovico, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/67661
Resumo: Nitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nomega-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis.
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spelling NO-mediated apoptosis in yeastApoptosisGlyceraldehyde-3-Phosphate DehydrogenasesHydrogen PeroxideNitric OxideOxidantsReactive Oxygen SpeciesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSignal TransductionYeast apoptosisS-nitrosationL-arginineglyceraldehyde-3-phosphate dehydrogenaseScience & TechnologyNitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nomega-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis.This work was supported by a grant from FCT-Fundação para a Ciência e a Tecnologia (POCI/BIA-BCM/57364/2004). B.A. has a fellowship from FCT (SFRH/BD/15317/2005). We are also grateful to FWF for a Lipotox grant to F.M. and B.M. and for grant no. S-9304-B05 to F.M. and S.B.The Company of BiologistsUniversidade do MinhoAlmeida, BrunoButtner, SabrinaOhlmeier, SteffenSilva, AlexandraMesquita, AnaMarques, Maria Belém Sousa SampaioOsório, Nuno S.Kollau, AlexanderMayer, BernhardLeão, CecíliaLaranjinha, JoãoRodrigues, Fernando José dos SantosMadeo, FrankLudovico, Paula2007-09-152007-09-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67661eng0021-95331477-913710.1242/jcs.01092617726063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:14Zoai:repositorium.sdum.uminho.pt:1822/67661Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:52.505218Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv NO-mediated apoptosis in yeast
title NO-mediated apoptosis in yeast
spellingShingle NO-mediated apoptosis in yeast
Almeida, Bruno
Apoptosis
Glyceraldehyde-3-Phosphate Dehydrogenases
Hydrogen Peroxide
Nitric Oxide
Oxidants
Reactive Oxygen Species
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Signal Transduction
Yeast apoptosis
S-nitrosation
L-arginine
glyceraldehyde-3-phosphate dehydrogenase
Science & Technology
title_short NO-mediated apoptosis in yeast
title_full NO-mediated apoptosis in yeast
title_fullStr NO-mediated apoptosis in yeast
title_full_unstemmed NO-mediated apoptosis in yeast
title_sort NO-mediated apoptosis in yeast
author Almeida, Bruno
author_facet Almeida, Bruno
Buttner, Sabrina
Ohlmeier, Steffen
Silva, Alexandra
Mesquita, Ana
Marques, Maria Belém Sousa Sampaio
Osório, Nuno S.
Kollau, Alexander
Mayer, Bernhard
Leão, Cecília
Laranjinha, João
Rodrigues, Fernando José dos Santos
Madeo, Frank
Ludovico, Paula
author_role author
author2 Buttner, Sabrina
Ohlmeier, Steffen
Silva, Alexandra
Mesquita, Ana
Marques, Maria Belém Sousa Sampaio
Osório, Nuno S.
Kollau, Alexander
Mayer, Bernhard
Leão, Cecília
Laranjinha, João
Rodrigues, Fernando José dos Santos
Madeo, Frank
Ludovico, Paula
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Almeida, Bruno
Buttner, Sabrina
Ohlmeier, Steffen
Silva, Alexandra
Mesquita, Ana
Marques, Maria Belém Sousa Sampaio
Osório, Nuno S.
Kollau, Alexander
Mayer, Bernhard
Leão, Cecília
Laranjinha, João
Rodrigues, Fernando José dos Santos
Madeo, Frank
Ludovico, Paula
dc.subject.por.fl_str_mv Apoptosis
Glyceraldehyde-3-Phosphate Dehydrogenases
Hydrogen Peroxide
Nitric Oxide
Oxidants
Reactive Oxygen Species
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Signal Transduction
Yeast apoptosis
S-nitrosation
L-arginine
glyceraldehyde-3-phosphate dehydrogenase
Science & Technology
topic Apoptosis
Glyceraldehyde-3-Phosphate Dehydrogenases
Hydrogen Peroxide
Nitric Oxide
Oxidants
Reactive Oxygen Species
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Signal Transduction
Yeast apoptosis
S-nitrosation
L-arginine
glyceraldehyde-3-phosphate dehydrogenase
Science & Technology
description Nitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nomega-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-15
2007-09-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/67661
url http://hdl.handle.net/1822/67661
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9533
1477-9137
10.1242/jcs.010926
17726063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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