NO-mediated apoptosis in yeast
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67661 |
Resumo: | Nitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nomega-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis. |
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7160 |
spelling |
NO-mediated apoptosis in yeastApoptosisGlyceraldehyde-3-Phosphate DehydrogenasesHydrogen PeroxideNitric OxideOxidantsReactive Oxygen SpeciesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSignal TransductionYeast apoptosisS-nitrosationL-arginineglyceraldehyde-3-phosphate dehydrogenaseScience & TechnologyNitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nomega-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis.This work was supported by a grant from FCT-Fundação para a Ciência e a Tecnologia (POCI/BIA-BCM/57364/2004). B.A. has a fellowship from FCT (SFRH/BD/15317/2005). We are also grateful to FWF for a Lipotox grant to F.M. and B.M. and for grant no. S-9304-B05 to F.M. and S.B.The Company of BiologistsUniversidade do MinhoAlmeida, BrunoButtner, SabrinaOhlmeier, SteffenSilva, AlexandraMesquita, AnaMarques, Maria Belém Sousa SampaioOsório, Nuno S.Kollau, AlexanderMayer, BernhardLeão, CecíliaLaranjinha, JoãoRodrigues, Fernando José dos SantosMadeo, FrankLudovico, Paula2007-09-152007-09-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67661eng0021-95331477-913710.1242/jcs.01092617726063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:14Zoai:repositorium.sdum.uminho.pt:1822/67661Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:52.505218Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
NO-mediated apoptosis in yeast |
title |
NO-mediated apoptosis in yeast |
spellingShingle |
NO-mediated apoptosis in yeast Almeida, Bruno Apoptosis Glyceraldehyde-3-Phosphate Dehydrogenases Hydrogen Peroxide Nitric Oxide Oxidants Reactive Oxygen Species Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Yeast apoptosis S-nitrosation L-arginine glyceraldehyde-3-phosphate dehydrogenase Science & Technology |
title_short |
NO-mediated apoptosis in yeast |
title_full |
NO-mediated apoptosis in yeast |
title_fullStr |
NO-mediated apoptosis in yeast |
title_full_unstemmed |
NO-mediated apoptosis in yeast |
title_sort |
NO-mediated apoptosis in yeast |
author |
Almeida, Bruno |
author_facet |
Almeida, Bruno Buttner, Sabrina Ohlmeier, Steffen Silva, Alexandra Mesquita, Ana Marques, Maria Belém Sousa Sampaio Osório, Nuno S. Kollau, Alexander Mayer, Bernhard Leão, Cecília Laranjinha, João Rodrigues, Fernando José dos Santos Madeo, Frank Ludovico, Paula |
author_role |
author |
author2 |
Buttner, Sabrina Ohlmeier, Steffen Silva, Alexandra Mesquita, Ana Marques, Maria Belém Sousa Sampaio Osório, Nuno S. Kollau, Alexander Mayer, Bernhard Leão, Cecília Laranjinha, João Rodrigues, Fernando José dos Santos Madeo, Frank Ludovico, Paula |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Almeida, Bruno Buttner, Sabrina Ohlmeier, Steffen Silva, Alexandra Mesquita, Ana Marques, Maria Belém Sousa Sampaio Osório, Nuno S. Kollau, Alexander Mayer, Bernhard Leão, Cecília Laranjinha, João Rodrigues, Fernando José dos Santos Madeo, Frank Ludovico, Paula |
dc.subject.por.fl_str_mv |
Apoptosis Glyceraldehyde-3-Phosphate Dehydrogenases Hydrogen Peroxide Nitric Oxide Oxidants Reactive Oxygen Species Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Yeast apoptosis S-nitrosation L-arginine glyceraldehyde-3-phosphate dehydrogenase Science & Technology |
topic |
Apoptosis Glyceraldehyde-3-Phosphate Dehydrogenases Hydrogen Peroxide Nitric Oxide Oxidants Reactive Oxygen Species Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Yeast apoptosis S-nitrosation L-arginine glyceraldehyde-3-phosphate dehydrogenase Science & Technology |
description |
Nitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nomega-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-15 2007-09-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67661 |
url |
http://hdl.handle.net/1822/67661 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0021-9533 1477-9137 10.1242/jcs.010926 17726063 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The Company of Biologists |
publisher.none.fl_str_mv |
The Company of Biologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132800274661376 |