DNA Interaction with Catanionic Vesicles
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10664 https://doi.org/10.1021/jp020391z |
Resumo: | DNA−cationic liposome complexes as possible vehicles for gene delivery is currently an important issue. In this work, the interaction between DNA and thermodynamically stable, spontaneously formed, catanionic vesicles with a net positive charge is studied. A phase map was drawn for the aqueous system of DNA and positively charged vesicles, composed of CTAB (cetyltrimethylammonium bromide) and SOS (sodium octyl sulfate), and showed, as expected, a strong associative phase behavior with the formation of a precipitate. A two-phase region was observed over all the studied concentrations. For DNA−surfactant mixing ratios, [DNA]/[S+] below 1.3 by charge, we found, by optical and electron microscopy, a coexistence between undisturbed vesicles and DNA−surfactant complexes. In samples with a higher excess of DNA, only DNA−vesicle complexes were observed in solution. The structure of these complexes was studied by both small-angle X-ray diffraction (SAXS) and cryogenic transmission electron microscopy (cryo-TEM), and a short-range lamellar structure composed of amphiphile bilayers with DNA molecules ordered and packed between these stacks was found. This type of structure has already been mentioned in the literature as being the most frequently found structure for DNA−liposome mixtures and shows that the vesicles we used, having major advantages with respect to preparation and stability, have similar behavior and can thus be successfully used as model systems. We observed, however, an interesting difference in comparison with previously studied systems. Thus, the addition of DNA in excess to the samples leads not to the coexistence of DNA−vesicle complexes and DNA, as observed before, but to a probable inclusion of DNA in excess in the complexes and therefore to a coexistence of complexes and anionic surfactant micelles expelled from the bilayers. |
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DNA Interaction with Catanionic VesiclesDNA−cationic liposome complexes as possible vehicles for gene delivery is currently an important issue. In this work, the interaction between DNA and thermodynamically stable, spontaneously formed, catanionic vesicles with a net positive charge is studied. A phase map was drawn for the aqueous system of DNA and positively charged vesicles, composed of CTAB (cetyltrimethylammonium bromide) and SOS (sodium octyl sulfate), and showed, as expected, a strong associative phase behavior with the formation of a precipitate. A two-phase region was observed over all the studied concentrations. For DNA−surfactant mixing ratios, [DNA]/[S+] below 1.3 by charge, we found, by optical and electron microscopy, a coexistence between undisturbed vesicles and DNA−surfactant complexes. In samples with a higher excess of DNA, only DNA−vesicle complexes were observed in solution. The structure of these complexes was studied by both small-angle X-ray diffraction (SAXS) and cryogenic transmission electron microscopy (cryo-TEM), and a short-range lamellar structure composed of amphiphile bilayers with DNA molecules ordered and packed between these stacks was found. This type of structure has already been mentioned in the literature as being the most frequently found structure for DNA−liposome mixtures and shows that the vesicles we used, having major advantages with respect to preparation and stability, have similar behavior and can thus be successfully used as model systems. We observed, however, an interesting difference in comparison with previously studied systems. Thus, the addition of DNA in excess to the samples leads not to the coexistence of DNA−vesicle complexes and DNA, as observed before, but to a probable inclusion of DNA in excess in the complexes and therefore to a coexistence of complexes and anionic surfactant micelles expelled from the bilayers.American Chemical Society2002-12-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10664http://hdl.handle.net/10316/10664https://doi.org/10.1021/jp020391zengThe Journal of Physical Chemistry B. 106:48 (2002) 12600-126071520-6106Dias, Rita S.Lindman, BjörnMiguel, Maria G.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-02-11T18:17:38Zoai:estudogeral.uc.pt:10316/10664Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:33.998148Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
DNA Interaction with Catanionic Vesicles |
title |
DNA Interaction with Catanionic Vesicles |
spellingShingle |
DNA Interaction with Catanionic Vesicles Dias, Rita S. |
title_short |
DNA Interaction with Catanionic Vesicles |
title_full |
DNA Interaction with Catanionic Vesicles |
title_fullStr |
DNA Interaction with Catanionic Vesicles |
title_full_unstemmed |
DNA Interaction with Catanionic Vesicles |
title_sort |
DNA Interaction with Catanionic Vesicles |
author |
Dias, Rita S. |
author_facet |
Dias, Rita S. Lindman, Björn Miguel, Maria G. |
author_role |
author |
author2 |
Lindman, Björn Miguel, Maria G. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dias, Rita S. Lindman, Björn Miguel, Maria G. |
description |
DNA−cationic liposome complexes as possible vehicles for gene delivery is currently an important issue. In this work, the interaction between DNA and thermodynamically stable, spontaneously formed, catanionic vesicles with a net positive charge is studied. A phase map was drawn for the aqueous system of DNA and positively charged vesicles, composed of CTAB (cetyltrimethylammonium bromide) and SOS (sodium octyl sulfate), and showed, as expected, a strong associative phase behavior with the formation of a precipitate. A two-phase region was observed over all the studied concentrations. For DNA−surfactant mixing ratios, [DNA]/[S+] below 1.3 by charge, we found, by optical and electron microscopy, a coexistence between undisturbed vesicles and DNA−surfactant complexes. In samples with a higher excess of DNA, only DNA−vesicle complexes were observed in solution. The structure of these complexes was studied by both small-angle X-ray diffraction (SAXS) and cryogenic transmission electron microscopy (cryo-TEM), and a short-range lamellar structure composed of amphiphile bilayers with DNA molecules ordered and packed between these stacks was found. This type of structure has already been mentioned in the literature as being the most frequently found structure for DNA−liposome mixtures and shows that the vesicles we used, having major advantages with respect to preparation and stability, have similar behavior and can thus be successfully used as model systems. We observed, however, an interesting difference in comparison with previously studied systems. Thus, the addition of DNA in excess to the samples leads not to the coexistence of DNA−vesicle complexes and DNA, as observed before, but to a probable inclusion of DNA in excess in the complexes and therefore to a coexistence of complexes and anionic surfactant micelles expelled from the bilayers. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10664 http://hdl.handle.net/10316/10664 https://doi.org/10.1021/jp020391z |
url |
http://hdl.handle.net/10316/10664 https://doi.org/10.1021/jp020391z |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
The Journal of Physical Chemistry B. 106:48 (2002) 12600-12607 1520-6106 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1817553876123910144 |