Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity

Detalhes bibliográficos
Autor(a) principal: Santos, Carmen
Data de Publicação: 2017
Outros Autores: Duarte, Sofia, Tedesco, Sara, Fevereiro, Pedro, Costa, Rita L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/13711
Resumo: The most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presented
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spelling Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunityCastaneaPhytophthora cinnamomiink diseaseplant biotic interactionsdigital PCRThe most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presentedFPLSRepositório da Universidade de LisboaSantos, CarmenDuarte, SofiaTedesco, SaraFevereiro, PedroCosta, Rita L.2017-06-02T13:20:06Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/13711engSantos C, Duarte S, Tedesco S, Fevereiro P and Costa RL (2017) Expression Profiling of Castanea Genes during Resistant and Susceptible Interactions with the Oomycete Pathogen Phytophthora cinnamomi Reveal Possible Mechanisms of Immunity. Front. Plant Sci. 8:515.10.3389/fpls.2017.00515info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:43:48Zoai:www.repository.utl.pt:10400.5/13711Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:59:39.427715Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
title Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
spellingShingle Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
Santos, Carmen
Castanea
Phytophthora cinnamomi
ink disease
plant biotic interactions
digital PCR
title_short Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
title_full Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
title_fullStr Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
title_full_unstemmed Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
title_sort Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
author Santos, Carmen
author_facet Santos, Carmen
Duarte, Sofia
Tedesco, Sara
Fevereiro, Pedro
Costa, Rita L.
author_role author
author2 Duarte, Sofia
Tedesco, Sara
Fevereiro, Pedro
Costa, Rita L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Carmen
Duarte, Sofia
Tedesco, Sara
Fevereiro, Pedro
Costa, Rita L.
dc.subject.por.fl_str_mv Castanea
Phytophthora cinnamomi
ink disease
plant biotic interactions
digital PCR
topic Castanea
Phytophthora cinnamomi
ink disease
plant biotic interactions
digital PCR
description The most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presented
publishDate 2017
dc.date.none.fl_str_mv 2017-06-02T13:20:06Z
2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/13711
url http://hdl.handle.net/10400.5/13711
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos C, Duarte S, Tedesco S, Fevereiro P and Costa RL (2017) Expression Profiling of Castanea Genes during Resistant and Susceptible Interactions with the Oomycete Pathogen Phytophthora cinnamomi Reveal Possible Mechanisms of Immunity. Front. Plant Sci. 8:515.
10.3389/fpls.2017.00515
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv FPLS
publisher.none.fl_str_mv FPLS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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