A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/13734 |
Resumo: | Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis. |
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A mouse model reproducing the pathophysiology of neonatal group B streptococcal infectionAnimalsBehavior, AnimalBody WeightHemolysin ProteinsInflammationMaze LearningMeningitisMeningitis, BacterialMiceMice, Inbred BALB CPerforinPregnancyPregnancy, AnimalStreptococcal InfectionsStreptococcus agalactiaeDisease Models, AnimalInfectious Disease Transmission, VerticalGroup B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis.Repositório Científico do Instituto Politécnico do PortoBonifácio Andrade, ElvaMagalhães, AnaPuga, AnaCosta, MadalenaBravo, JoanaPortugal, Camila CabralRibeiro, AdíliaCorreia-Neves, MargaridaFaustino, AugustoFiron, ArnaudTrieu-Cuot, PatrickSummavielle, TeresaFerreira, Paula2019-05-17T16:52:06Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/13734engAndrade, E.B., Magalhães, A., Puga, A. et al. A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection (2018). Nat Commun 9, 3138. https://doi.org/10.1038/s41467-018-05492-y10.1038/s41467-018-05492-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:53:11Zoai:recipp.ipp.pt:10400.22/13734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:33:36.236885Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
title |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
spellingShingle |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection Bonifácio Andrade, Elva Animals Behavior, Animal Body Weight Hemolysin Proteins Inflammation Maze Learning Meningitis Meningitis, Bacterial Mice Mice, Inbred BALB C Perforin Pregnancy Pregnancy, Animal Streptococcal Infections Streptococcus agalactiae Disease Models, Animal Infectious Disease Transmission, Vertical |
title_short |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
title_full |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
title_fullStr |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
title_full_unstemmed |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
title_sort |
A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection |
author |
Bonifácio Andrade, Elva |
author_facet |
Bonifácio Andrade, Elva Magalhães, Ana Puga, Ana Costa, Madalena Bravo, Joana Portugal, Camila Cabral Ribeiro, Adília Correia-Neves, Margarida Faustino, Augusto Firon, Arnaud Trieu-Cuot, Patrick Summavielle, Teresa Ferreira, Paula |
author_role |
author |
author2 |
Magalhães, Ana Puga, Ana Costa, Madalena Bravo, Joana Portugal, Camila Cabral Ribeiro, Adília Correia-Neves, Margarida Faustino, Augusto Firon, Arnaud Trieu-Cuot, Patrick Summavielle, Teresa Ferreira, Paula |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Bonifácio Andrade, Elva Magalhães, Ana Puga, Ana Costa, Madalena Bravo, Joana Portugal, Camila Cabral Ribeiro, Adília Correia-Neves, Margarida Faustino, Augusto Firon, Arnaud Trieu-Cuot, Patrick Summavielle, Teresa Ferreira, Paula |
dc.subject.por.fl_str_mv |
Animals Behavior, Animal Body Weight Hemolysin Proteins Inflammation Maze Learning Meningitis Meningitis, Bacterial Mice Mice, Inbred BALB C Perforin Pregnancy Pregnancy, Animal Streptococcal Infections Streptococcus agalactiae Disease Models, Animal Infectious Disease Transmission, Vertical |
topic |
Animals Behavior, Animal Body Weight Hemolysin Proteins Inflammation Maze Learning Meningitis Meningitis, Bacterial Mice Mice, Inbred BALB C Perforin Pregnancy Pregnancy, Animal Streptococcal Infections Streptococcus agalactiae Disease Models, Animal Infectious Disease Transmission, Vertical |
description |
Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z 2019-05-17T16:52:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/13734 |
url |
http://hdl.handle.net/10400.22/13734 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Andrade, E.B., Magalhães, A., Puga, A. et al. A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection (2018). Nat Commun 9, 3138. https://doi.org/10.1038/s41467-018-05492-y 10.1038/s41467-018-05492-y |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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