Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34027 |
Resumo: | The use of small animals in the preclinical research field has been very important to the investigation and development of diagnostic and treatment agents, as new drugs, action mechanisms of certain pathogenic agents, and the discovery of some deseases’ origin. In order to understand the organismtriggered responses to the referred processes, techniques and functional imaging systems, like Positron Emission Tomography (PET), are widely used. To perform the imaging procedures, mice have to be anesthetized, which can lead to changes in their physiological parameters and vital signs. Thus, this work has as main goal the development of a system for monitoring the mice’ vital signs in real time, during PET imaging procedures, performed with easyPET system, in order to ensure the animals well-being over the imaging acquisition. The considered vital signs were: heart rate, respiratory rate, oxygen saturation, and body temperature. This study evaluates a set of sensors (piezoelectric sensor, optical module, non-invasive ECG, and thermistor) for the different animal’s vital parameters measurement, of which stands out the developed hardware, as well as the software necessary to control the several devices. The several sensors were tested, at an initial phase, in human volunteers, being later implemented in the bed prototype, which as been also tested in a real application, with mice. In order to test the developed system, the mice were submitted to different anesthesias, in order to validate the system, through the comparison of the obtained values with ones well established in literature, for a similar anesthesia, and the system was also tested in an unknown situation. In the system validation phase, the mice were anesthetized with sevoflurane, and the obtained values where compared to the ones from isoflurane. Thus, at a sevoflurane’s concentration of 1.5%, the computed values to the heart rate were around the 388 bpm, the respiratory rate was about 124 rpm, and the SpO₂ was 88.9%. These values are considered coherent, since they are very close to the ones established for isoflurane. The system was also tested with an injectable anesthesia (ketamine+ chlorpromazine), which does not have reported values for the vital signs. In this case, the obtained values for the heart rate were between 321 and 383 bpm, for the respiratory rate varied from 182 to 226 rpm, and the SpO₂ was above 82%. |
id |
RCAP_19312caacb9cec953e7ba665ca567b4a |
---|---|
oai_identifier_str |
oai:ria.ua.pt:10773/34027 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systemsSmall animals imagingPositron Emission TomographyAnesthesiaHeart rateRespiratory rateBody temperatureOxygen saturationThe use of small animals in the preclinical research field has been very important to the investigation and development of diagnostic and treatment agents, as new drugs, action mechanisms of certain pathogenic agents, and the discovery of some deseases’ origin. In order to understand the organismtriggered responses to the referred processes, techniques and functional imaging systems, like Positron Emission Tomography (PET), are widely used. To perform the imaging procedures, mice have to be anesthetized, which can lead to changes in their physiological parameters and vital signs. Thus, this work has as main goal the development of a system for monitoring the mice’ vital signs in real time, during PET imaging procedures, performed with easyPET system, in order to ensure the animals well-being over the imaging acquisition. The considered vital signs were: heart rate, respiratory rate, oxygen saturation, and body temperature. This study evaluates a set of sensors (piezoelectric sensor, optical module, non-invasive ECG, and thermistor) for the different animal’s vital parameters measurement, of which stands out the developed hardware, as well as the software necessary to control the several devices. The several sensors were tested, at an initial phase, in human volunteers, being later implemented in the bed prototype, which as been also tested in a real application, with mice. In order to test the developed system, the mice were submitted to different anesthesias, in order to validate the system, through the comparison of the obtained values with ones well established in literature, for a similar anesthesia, and the system was also tested in an unknown situation. In the system validation phase, the mice were anesthetized with sevoflurane, and the obtained values where compared to the ones from isoflurane. Thus, at a sevoflurane’s concentration of 1.5%, the computed values to the heart rate were around the 388 bpm, the respiratory rate was about 124 rpm, and the SpO₂ was 88.9%. These values are considered coherent, since they are very close to the ones established for isoflurane. The system was also tested with an injectable anesthesia (ketamine+ chlorpromazine), which does not have reported values for the vital signs. In this case, the obtained values for the heart rate were between 321 and 383 bpm, for the respiratory rate varied from 182 to 226 rpm, and the SpO₂ was above 82%.O uso de pequenos animais em investigação pré-clínica tem sido particularmente importante no que diz respeito à investigação e desenvolvimento de agentes de diagnóstico e tratamento, como novos medicamentos, mecanismos de ação de um determinado agente patogénico, e descoberta da origem de certas doenças. De forma a perceber as respostas desencadeadas pelo organismo aos processos referidos, são muitas vezes utilizadas técnicas e sistemas de imagem funcional, como a Tomografia por Emissão de Positrões (PET). Para a realização dos procedimentos de imagem, é necessário anestesiar os ratinhos, o que pode desencadear alterações dos seus parâmetros fisiológicos e sinais vitais. Assim, este trabalho tem como principal objetivo desenvolver um sistema para monitorização em tempo real dos sinais vitais de ratinhos de laboratório durante o exame PET, realizado com o sistema easyPET, de forma a garantir o seu bem-estar durante todo o procedimento. Os sinais vitais considerados neste trabalho foram: batimento cardíaco, frequência respiratória, saturação de oxigénio (SpO₂) e temperatura corporal. Este estudo avalia um conjunto de soluções (sensor piezoelétrico, módulo ótico, ECG, e termistor), para a medição dos diferentes parâmetros fisiológicos dos pequenos animais, destacando-se o desenvolvimento de hardware associado, bem como o software de controlo dos diversos sistemas desenvolvidos. Os diversos sensores foram testados numa fase inicial em indivíduos, tendo sido posteriormente implementados no protótipo da caminha, o qual foi igualmente testado em situação real de aplicação, com ratinhos. Na aplicação deste conjunto de sensores em ratinhos foram variadas as condições de anestesia, de forma a conseguirmos validar o nosso sistema, através da comparação dos valores obtidos com valores já estabelecidos na literatura, e, ainda, testar o sistema numa situação desconhecida. Na fase de validação do sistema, recorreu-se ao uso de anestesia gasosa (sevoflurano) comparando os valores obtidos com os reportados para uma outra anestesia, isoflurano. Assim, para uma concentração de 1.5%, para o sevoflurano, os valores do batimento cardíaco situaram-se nos 388 bpm, a frequência respiratória nas 124 rpm, e a SpO₂ a 88.9%. Estes valores são considerados coerentes, visto que se encontram muito próximos dos estabelecidos para a anestesia com isoflurano. O sistema foi também testado com uma anestesia injetável (ketamine+ chlorpromazine), para a qual não existem valores para os sinais vitais reportados na literatura. Neste caso, os valores obtidos para o batimento cardíaco foram de 321 a 383 bpm, para a frequência respiratória de 182-226 rpm, e a SpO₂ foi superior a 82%.2023-11-24T00:00:00Z2021-11-19T00:00:00Z2021-11-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/34027engOliveira, Regina Gapo deinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:05:27Zoai:ria.ua.pt:10773/34027Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:21.012348Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
title |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
spellingShingle |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems Oliveira, Regina Gapo de Small animals imaging Positron Emission Tomography Anesthesia Heart rate Respiratory rate Body temperature Oxygen saturation |
title_short |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
title_full |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
title_fullStr |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
title_full_unstemmed |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
title_sort |
Small animals’ vital signs monitoring: development of an integrated system for preclinical PET systems |
author |
Oliveira, Regina Gapo de |
author_facet |
Oliveira, Regina Gapo de |
author_role |
author |
dc.contributor.author.fl_str_mv |
Oliveira, Regina Gapo de |
dc.subject.por.fl_str_mv |
Small animals imaging Positron Emission Tomography Anesthesia Heart rate Respiratory rate Body temperature Oxygen saturation |
topic |
Small animals imaging Positron Emission Tomography Anesthesia Heart rate Respiratory rate Body temperature Oxygen saturation |
description |
The use of small animals in the preclinical research field has been very important to the investigation and development of diagnostic and treatment agents, as new drugs, action mechanisms of certain pathogenic agents, and the discovery of some deseases’ origin. In order to understand the organismtriggered responses to the referred processes, techniques and functional imaging systems, like Positron Emission Tomography (PET), are widely used. To perform the imaging procedures, mice have to be anesthetized, which can lead to changes in their physiological parameters and vital signs. Thus, this work has as main goal the development of a system for monitoring the mice’ vital signs in real time, during PET imaging procedures, performed with easyPET system, in order to ensure the animals well-being over the imaging acquisition. The considered vital signs were: heart rate, respiratory rate, oxygen saturation, and body temperature. This study evaluates a set of sensors (piezoelectric sensor, optical module, non-invasive ECG, and thermistor) for the different animal’s vital parameters measurement, of which stands out the developed hardware, as well as the software necessary to control the several devices. The several sensors were tested, at an initial phase, in human volunteers, being later implemented in the bed prototype, which as been also tested in a real application, with mice. In order to test the developed system, the mice were submitted to different anesthesias, in order to validate the system, through the comparison of the obtained values with ones well established in literature, for a similar anesthesia, and the system was also tested in an unknown situation. In the system validation phase, the mice were anesthetized with sevoflurane, and the obtained values where compared to the ones from isoflurane. Thus, at a sevoflurane’s concentration of 1.5%, the computed values to the heart rate were around the 388 bpm, the respiratory rate was about 124 rpm, and the SpO₂ was 88.9%. These values are considered coherent, since they are very close to the ones established for isoflurane. The system was also tested with an injectable anesthesia (ketamine+ chlorpromazine), which does not have reported values for the vital signs. In this case, the obtained values for the heart rate were between 321 and 383 bpm, for the respiratory rate varied from 182 to 226 rpm, and the SpO₂ was above 82%. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-19T00:00:00Z 2021-11-19 2023-11-24T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/34027 |
url |
http://hdl.handle.net/10773/34027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137708737560576 |